First record of Trochulus clandestinus (Hartmann, 1821) in Austria (Gastropoda: Eupulmonata: Hygromiidae)

Duda, Michael; Schindelar, Julia; Macek, Oliver; Eschner, Anita; Kruckenhauser, Luise

doi:10.5817/mab2017-16-37

Cited by 6 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Full-length COI-5P DNA barcodes were amplified using primer sets LCO1490/ HCO2198 [44] and jgLCO1490/jgHCO2198 [45]. Even though additional primer sets were initially tested [46,47], all DNA barcodes were successfully amplified with the use of jgLCO1490/jgHCO2198 primer set with the exception of the specimens identified as Mytilus galloprovincialis which were amplified with LCO1490/HCO2198 primer set. 20 µL polymerase chain reactions (PCR) mixture contained 1× DreamTaq TM Reaction Buffer (containing 2 mM MgCl 2 , Thermo Scientific, Waltham, Massachusetts, USA), 0.2 mM dNTP mix (Qiagen), 0.5 µM of each primer, 1.0 U DreamTaq polymerase (Thermo Scientific) and 3 µL of DNA eluate (10-fold dilution of the second eluate).…”

Section: Methodsmentioning

confidence: 99%

DNA Barcoding of Marine Mollusks Associated with Corallina officinalis Turfs in Southern Istria (Adriatic Sea)

et al. 2021

View full text Add to dashboard Cite

Presence of mollusk assemblages was studied within red coralligenous algae Corallina officinalis L. along the southern Istrian coast. C. officinalis turfs can be considered a biodiversity reservoir, as they shelter numerous invertebrate species. The aim of this study was to identify mollusk species within these settlements using DNA barcoding as a method for detailed identification of mollusks. Nine locations and 18 localities with algal coverage range above 90% were chosen at four research areas. From 54 collected samples of C. officinalis turfs, a total of 46 mollusk species were identified. Molecular methods helped identify 16 gastropod, 14 bivalve and one polyplacophoran species. COI sequences for two bivalve species (Musculus cf. costulatus (Risso, 1826) and Gregariella semigranata (Reeve, 1858)) and seven gastropod species (Megastomia winfriedi Peñas & Rolán, 1999, Eatonina sp. Thiele, 1912, Eatonina cossurae (Calcara, 1841), Crisilla cf. maculata (Monterosato, 1869), Alvania cf. carinata (da Costa, 1778), Vitreolina antiflexa (Monterosato, 1884) and Odostomia plicata (Montagu, 1803)) represent new BINs in BOLD database. This study contributes to new findings related to the high biodiversity of mollusks associated with widespread C. officinalis settlements along the southern coastal area of Istria.

show abstract

Section: Methodsmentioning

confidence: 99%

DNA Barcoding of Marine Mollusks Associated with Corallina officinalis Turfs in Southern Istria (Adriatic Sea)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The Qubit TM dsDNA HS Assay Kit with the associated standard protocol was used. To amplify the DNA barcoding fragment of the mitochondrial cytochrome oxidase 1 gene, the primer pair LCO1490_Mol1/HCO2198_Mol1 (Duda et al, 2017) was used. PCRs were performed on a Master Gradient thermocycler (Eppendorf) in 25 ml elution buffer with 5 ml template DNA, using the QIAGEN Multiplex PCR Kit with 0.5 mM of each primer.…”

Section: Methodsmentioning

confidence: 99%

Site-specific attachment of Anodonta anatina (Bivalvia: Unionidae) glochidia on two new fish hosts translocated in Lake Trasimeno (Italy)

Ahnelt

Leister²,

Kruckenhauser³

et al. 2023

Knowl. Manag. Aquat. Ecosyst.

Self Cite

View full text Add to dashboard Cite

To complete their life cycle, freshwater mussels of the order Unionida depend on fishes as hosts for their obligatory parasitic larval phase, the glochidium. Here we report the first documentation of gobioid fishes as hosts of glochidia of unionid mussels in the wild in Italy and in southern Europe (outside of the Danube drainage area). We also examined the pattern of the attached glochidia. A recent review reported 326 fish species as suitable hosts for unionids, but only eight (2.5%) of these were Gobioidei. Host identification and the documentation of their benefits or threats for the interaction of the mussels with their hosts is important for conservation of freshwater mussels. But the co-existence of mussels with non-native fish species is only poorly understood, including the compatibility of these two groups. Knipowitschia panizzae and Pomatoschistus canestrinii, two translocated sand gobies, were sampled in 2022 at the east shore of Lake Trasimeno (Italy). Two species of anodontine mussels were present at this locality, the native Anodonta anatina and the translocated Sinanodonta woodiana. Genetic data revealed that both sand gobies hosted glochidia only of A. anatina but not of S. woodiana, possibly because of seasonal bias. About 50% of the specimens of both fish species were infested. The paired fins were the most prominent targets. The examined individuals of both sand gobies carried only few glochidia (max. 5). Nevertheless, the two species showed a divergent pattern of glochidia attachment.

show abstract

Speciation in sympatric species of land snails from the genus Trochulus (Gastropoda, Hygromiidae)

et al. 2020

View full text Add to dashboard Cite

The identification and designation of land snail species in the genus Trochulus on the basis of shell characteristics are problematic because of their great phenotypic plasticity. Some genetic analyses have proved inconclusive, with much variation within populations and apparent gene flow among them. We examined this issue by morphometric and molecular approaches on the morphologically similar species T. coelomphala, T. hispidus and T. striolatus, co‐occurring in the Alpenvorland of Germany. While these species differed in shell and reproductive system morphology, there were forms that turned out intermediate in shell characters between T. coelomphala and T. hispidus but had genital morphology similar to T. coelomphala. Phylogenetic analysis, however, showed that these forms clustered neither with T. coelomphala nor T. hispidus but are sister to T. striolatus from the same region, which suggests that they evolved by way of sympatric speciation. Further, these analyses suggest that T. coelomphala diverged within T. hispidus; a crossing experiment indicated that they were interfertile. Expanding the study to include all available Trochulus sequences enabled us to infer evolutionary relationships between them and showed that T. hispidus is polyphyletic. Some Trochulus samples of one nominal species were grouped within others. The combination of phenotypic plasticity and possible mitochondrial DNA introgression illustrates the complex nature of evolutionary processes and the need for caution in the application of traditional taxonomic practice.

show abstract

First record of Trochulus clandestinus (Hartmann, 1821) in Austria (Gastropoda: Eupulmonata: Hygromiidae)

Cited by 6 publications

References 12 publications

DNA Barcoding of Marine Mollusks Associated with Corallina officinalis Turfs in Southern Istria (Adriatic Sea)

DNA Barcoding of Marine Mollusks Associated with Corallina officinalis Turfs in Southern Istria (Adriatic Sea)

Site-specific attachment of Anodonta anatina (Bivalvia: Unionidae) glochidia on two new fish hosts translocated in Lake Trasimeno (Italy)

Speciation in sympatric species of land snails from the genus Trochulus (Gastropoda, Hygromiidae)

Contact Info

Product

Resources

About