First Report and Prevalence of <i>Grapevine fleck virus</i> in Grapevines (<i>Vitis vinifera</i>) in Canada

Poojari, Sudarsana; Lowery, Tom; Rott, M.; Schmidt, Anna-mary; DeLury, Naomi C.; Boulé, J.; Úrbez-Torres, J. R.

doi:10.1094/pdis-10-15-1212-pdn

Cited by 9 publications

(7 citation statements)

References 3 publications

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“…GFkV (genus Maculavirus) is widespread worldwide [95]. Recently, it was detected in the territories of Bosnia and Herzegovina [96], the USA [97], Canada [98], Macedonia [99], the Republic of Korea [100], and the United Kingdom [101], but it is known to also be present in other countries. This is the first time that this virus has been described in the Russian territory [29].…”

Section: Validation Of the Results Of Srna Hts By Rt-pcr And The Phylogenetic Analysis Of Detected Virusesmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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The use of high-throughput sequencing (HTS) technology has led to significant progress in the identification of many viruses and their genetic variants. In this study, we used the HTS platform to sequence small RNAs (sRNAs) of grapevine to study the virome. Isolation of RNA was performed using symptomatic grapevines collected from commercial vineyards in Krasnodar Krai in 2017–2018. To determine the viromes of vineyards, we used an integrated approach that included a bioinformatic analysis of the results of sRNA HTS and the molecular method RT-PCR, which made it possible to identify 13 viruses and 4 viroids. Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine Syrah Virus-1 (GSyV-1), Raspberry bushy dwarf virus (RBDV), Australian grapevine viroid (AGVd), and Grapevine yellow speckle viroid 2 (GYSVd-2) were identified for the first time in Russia. Out of 38 samples analyzed, 37 had mixed infections with 4–11 viruses, indicating a high viral load. Analysis of the obtained sequences of fragments of virus genomes made it possible to identify recombination events in GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4, GVT, GPGV, GRSPaV, GVA, and GFLV. The obtained results indicate a wide spread of the viruses and a high genetic diversity in the vineyards of Krasnodar Krai and emphasize the urgent need to develop and implement long-term strategies for the control of viral grapevine diseases.

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Section: Validation Of the Results Of Srna Hts By Rt-pcr And The Phylogenetic Analysis Of Detected Virusesmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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“…GFkV was detected in 21.8% of the samples with the other three viruses being detected much more rarely. GFkV was also commonly detected in British Columbia, with 29.7% of the samples tested positive for this virus [ 39 ]. The real economic impact of GFkV, and the related viruses, awaits to be seen.…”

Section: Discussionmentioning

confidence: 99%

Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario

Xiao

Shabanian

Moore

et al. 2018

Virol J

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BackgroundIn recent years, the Ontario grape and wine industry has experienced outbreaks of viral diseases across the province. Little is known about the prevalence of viruses and viral diseases in Ontario. Since 2015, we have conducted large-scale surveys for major viruses in commercial wine grapes in order to obtain a comprehensive understanding of the prevalence and severity of viral diseases in Ontario.MethodsA total of 657 composite leaf samples representing 3285 vines collected from 137 vine blocks of 33 vineyards from three appellations: Niagara Peninsula, Lake Erie North Shore and Prince Edward County. These samples covered six major red cultivars and five major white grape cultivars. Using a multiplex RT-PCR format, we tested these samples for 17 viruses including those involved in all major viral diseases of the grapevine, such as five grapevine leafroll-associated viruses (GLRaV-1, 2, 3, 4, 7), grapevine red blotch virus (GRBV), grapevine Pinot gris virus (GPGV), grapevine rupestris stem sitting-associated virus (GRSPaV), grapevine virus A (GVA), grapevine virus B (GVB), grapevine fleck virus (GFkV), arabis mosaic virus (ArMV), tomato ringspot virus (ToRSV), trapevine fanleaf virus (GFLV), among others.ResultsFourteen of the 17 viruses were detected from these samples and the predominant viruses are GRSPaV, GLRaV-3, GFkV, GPGV and GRBaV with an incidence of 84.0, 47.9, 21.8, 21.6 and 18.3%, respectively. As expected, mixed infections with multiple viruses are common. 95.6% of the samples included in the survey were infected with at least one virus; 67% of the samples with 2–4 viruses and 4.7% of the samples with 5–6 viruses. The major grape cultivars all tested positive for these major viruses. The results also suggested that the use of infected planting material may have been one of the chief factors responsible for the recent outbreaks of viral diseases across the province.ConclusionsThis is the first such comprehensive survey for grapevine viruses in Ontario and one of the most extensive surveys ever conducted in Canada. The recent outbreaks of viral diseases in Ontario vineyards were likely caused by GLRaV-3, GRBV and GPGV. Findings from this survey provides a baseline for the grape and wine industry in developing strategies for managing grapevine viral diseases in Ontario vineyards.

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“…Since no Xiphinema spp., the vectors of both viruses, have been detected in this and in previous studies in BiH [ 74 ], the possible route for their introduction in the country might again be the use of infected propagation material. As far as GFkV is concerned, all the BiH isolates grouped together in the same subgroup with a few virus isolates from Hungary, which could be related to an exchange of infected propagation material, since the vector for this virus is not known [ 36 , 86 ]. Based on these findings, if we exclude in the future the use of infected propagation material, further spread of GLRaV-1 and GLRaV-3 might be facilitated due to the presence of mixed infections and of the mealybugs and scale vectors that could act as potential vectors, while the risk of GFLV, ArMV and GFkV spread would be less envisaged, as no vectors are present in this area.…”

Section: Discussionmentioning

confidence: 99%

“…ArMV is transmitted by another ectoparasitic soilborne nematode, Xiphinema diversicaudatum [ 35 ]. The primary spread of GFkV is known to occur through infected propagation material and no vector has yet been reported for this virus [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina

et al. 2021

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The sanitary status of grapevines has not yet been considered sufficiently in vineyards throughout Bosnia and Herzegovina (BiH). An extensive survey of five major grapevine viruses in the country was carried out in 2019. A total of 630 samples from the two dominant autochthonous cultivars, named Žilavka and Blatina, were tested by DAS-ELISA for the presence of grapevine leafroll-associated viruses (GLRaV-1 and 3), grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). Eighty-eight % of the samples were positive for at least one virus, and all five viruses were detected, thought with different incidence, i.e. GLRaV-3 (84%), GFLV (43%), GLRaV-1 (14%), GFkV (10%) and ArMV (0.2%). The majority of infected plants (about 75%) were asymptomatic. Specific virus symptoms were observed in the remaining infected plants, together with the reported GLRaV vectors, Planococcus ficus and Parthenolecanium corni, while nematodes of the Xiphinema genus were not found in the GFLV- or ArMV-infected vineyards. The GLRaV-3 CP phylogenetic analyses showed 75–100% nucleotide identity between the BiH and reference isolates, and the BiH isolates clustered into the major group. The dNS/dS ratio indicated a negative selection of the virus population, and the lack of geographical structuring within the population was observed. In addition, putative GLRaV-3 recombinants with breakpoints in the 5’ of the CP gene were detected, while no recombinant strains were identified for the other four viruses. The obtained results indicate a deteriorated sanitary status of the cultivated grapevines, the prevalence and intraspecies genetic diversity of GLRaV-3 throughout the country. The establishment of certified grapevine material and adequate virus vector control is therefore of primary importance to prevent further spread of these viruses. This study presents the results of the first molecular characterisation of grapevine viruses in Bosnia and Herzegovina.

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First Report and Prevalence of Grapevine fleck virus in Grapevines (Vitis vinifera) in Canada

Cited by 9 publications

References 3 publications

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario

Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina

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