First report of a ‘<i>Candidatus</i> Phytoplasma asteris‘‐related strain (16SrI‐B subgroup) associated with witches' broom disease in <i>Cucurbita pepo</i> in India

Rao, G. P.; Gopala,; Goel, Sonia; Rao, A. V.

doi:10.5197/j.2044-0588.2017.035.033

Cited by 12 publications

(4 citation statements)

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“…Phytoplasma diseases continue to be a concern for tomato, brinjal, potato, cucumber, squash, cabbage, spinach, lettuce, faba bean and chili in Iran where they are associated mainly with 16SrII and 16SrVI phytoplasmas (Zibadoost et al, 2016) but also ribosomal groups 16SrI, 16SrIX, and 16SrXII were reported in various vegetable crops cultivations. 16SrI and 16SrII groups are found to be causing severe yield loss in squash in India and Iran (Hosseini et al, 2011; Salehi et al, 2015; Rao et al, 2017b). Phytoplasma diseases associated with different symptoms in onion and garlic crops were associated with the presence of 16SrI and 16SrIII groups in America and Europe (Vibio et al, 1995; Khadhair et al, 2002; Galdeano et al, 2004; Lee et al, 2011; Mollov et al, 2014) where diverse vegetable crops resulted infected by phytoplasmas in groups 16SrXIII, 16SrXV, 16SrXVIII, and 16SrXXXI.…”

Section: Phytoplasma Diseases In Vegetable Speciesmentioning

confidence: 99%

Global Status of Phytoplasma Diseases in Vegetable Crops

et al. 2019

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The presence of phytoplasmas and their associated diseases is an emerging threat to vegetable production which leads to severe yield losses worldwide. Phytoplasmas are phloem-limited pleomorphic bacteria lacking the cell wall, mainly transmitted through leafhoppers but also by plant propagation materials and seeds. Phytoplasma diseases of vegetable crops are characterized by symptoms such as little leaves, phyllody, flower virescence, big buds, and witches’ brooms. Phytoplasmas enclosed in at least sixteen different ribosomal groups infecting vegetable crops have been reported thus far across the world. The aster yellows phytoplasma group (16SrI) is presently the prevalent, followed by the peanut witches’ broom (16SrII). Wide and overlapping crop and non-crop host ranges of phytoplasmas, polyphagous insect vectors, limited availability of resistance sources and unavailability of environmentally safe chemical control measures lead to an arduous effort in the management of these diseases. The most feasible control of vegetable phytoplasma diseases is a consequence of the development and implementation of integrated disease management programs. The availability of molecular tools for phytoplasma identification at the strain level greatly facilitated this kind of approach. It is moreover essential to understand the molecular basis of phytoplasma-vector interaction, epidemiology and other factors involved in disease development in order to reduce the disease outbreaks. Information on the knowledge about the most widespread phytoplasma diseases in vegetable crops is reviewed here in a comprehensive manner.

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Section: Phytoplasma Diseases In Vegetable Speciesmentioning

confidence: 99%

Global Status of Phytoplasma Diseases in Vegetable Crops

et al. 2019

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“…Phytoplasma diseases cause significant economic losses in numerous Cucurbitaceae -related vegetable crops, including C. maxima (Giant pumpkin), C. mixta (winter Squash), C. pepo (summer squash) (Rao et al 2017), Lagenaria leucantha (Bottle gourd) (Ashwathappa et al 2020; Tripathi et al 2017), C. moschata (Butternut Pumpkin) (Montano et al 2006; Xu et al 2021), Momordica charantia (Bitter gourd) (Montano et al 2007; Venkataravanappa et al 2017). However, there is no information is available for phytoplasma infection in cucumber in India.…”

Section: Resultsmentioning

confidence: 99%

“…All over the world, phytoplasmas cause a multitude of diseases in several hundred crop species including fruits, vegetables, cereals and trees (McCoy et al 1989; Lee et al 2000). Many cucurbits belongs to the Cucurbitaceous family have been infected with different phytoplasma has been well documented in different parts of the world that includes witches’ broom of Cucurbita pepo in India (Rao et al 2017), cucumber phyllody in Iran (Hosseini et al 2015) and cucumber yellows (Valiunas et al 2017). Yellows of C. maxima , (McCoy et al, 1989), C. moschata (Montano et al 2006), Luffa cylindrica L. (Lee et al 1993; Gundersen et al 1994), Momordica charantia L. (Alves et al 2017), Sechium edule (Jacquin) Swartz (Villalobos et al 2002), Sicana odorifera (Montano et al 2007), Trichosanthes cucumerina (Weng et al 2021) and C. moschata L., (Xu et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…pepo (summer squash)(Rao et al 2017), Lagenaria leucantha (Bottle gourd)(Ashwathappa et al 2020;Tripathi et al 2017), C. moschata (Butternut Pumpkin)(Montano et al 2006; Xu et al 2021), Momordica charantia (Bitter gourd)(Montano et al 2007;Venkataravanappa et al 2017). However, there is no information is available for phytoplasma infection in cucumber in India.…”

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Detection and molecular characterization of a phytoplasma associated with Cucumber (Cucumis sativus) and its first report from India

Muttappagol

Hiremath

et al. 2022

Preprint

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Cucumber (Cucumis sativus) plants exhibiting typical phyllody symptoms were collected from farmers field of Chintamani, Chickballapur districts of Karnataka (India). Disease incidence of phyllody was 2-3%. The etiology of the cucumber phyllody phytoplasma (CuPP) was confirmed by amplifying 16S rRNA gene from symptomatic plants using PCR followed by nested PCR using universal primers pairs P1/P7 and R16F2n/R16R2. After general detection the non-ribosomal SecY and rp (ribosomal protein) genes was amplified using specific primers. The PCR amplified products 16SrRNA (1.2 kb), SecY (1.6 kb) rp gene (1.2 kb) was cloned and sequenced. Sequence and phylogenetic analyses of 16S rRNA, secY and rp genes revealed that the detected phytoplasma is a member of the 16SrI group (Candidatus Phytoplasma asteris). Further on the basis of computer-simulated RFLP (= in silico RFLP) analysis of amplified F2n/R2 region of 16S rRNA gene indicates that, the detected phytoplasma was belongs to the subgroup X (16SrII-X). This is the first report on phytoplasma associated with phyllody disease of cucumber in India.

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