First report of antiviral activity of nordihydroguaiaretic acid against Fort Sherman virus (Orthobunyavirus)

Martinez, Florencia; Mugas, María Laura; Aguilar, Javier; Marioni, Juliana; Contigiani, Marta Silvia; Montoya, Susana C. Núñez; Konigheim, Brenda

doi:10.1016/j.antiviral.2020.104976

Cited by 4 publications

(3 citation statements)

References 47 publications

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“…NDGA (nordihydroguaiaretic acid) and caffeic acid (3,4-dihydroxycinnamic acid) are natural phenolic compounds with the ability to reduce ferric to catalytically inactive ferrous [ 15 , 16 ]. These compounds were also found to be inhibitors of a wide range of lipoxygenases [ 17 , 18 ]. In the present study, both compounds exhibited strong inhibitions of porcine 12-LOXcd.…”

Section: Resultsmentioning

confidence: 99%

Recombinant Porcine 12-Lipoxygenase Catalytic Domain: Effect of Inhibitors, Selectivity of Substrates and Specificity of Oxidation Products of Linoleic Acid

Liu

et al. 2022

Foods

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Lipoxygenase (LOX) is a major endogenous enzyme for the enzymatic oxidation of lipids during meat storage and meat product manufacturing. In the present work, some characteristics, i.e., effects of inhibitors, selectivity of substrates and specificity of oxidation products, were studied using recombinant porcine 12-lipoxygenase catalytic domain (12-LOXcd). Several familiar inhibitors were found inhibit the activity of recombinant porcine 12-LOXcd;nordihydroguaiaretic acid demonstrated the strongest inhibitory effect. The enzyme could oxygenate common polyunsaturated fatty acids, and showed the highest affinity to linoleic acid (LA), followed by arachidonic acid (AA), linolenic acid (LN) and docosahexaenoic acid (DHA). Under the action of porcine 12-LOXcd, LA was oxidized into four hydroxyoctadecadienoic acid (HODE) isomers, i.e., 13-Z,E-HODE, 13-E,E-HODE, 9-Z,E-HODE and 9-E,E-HODE. Variation of pH not only affected the yield of LA oxidation products, but also the distribution of HODE isomers. These results indicated that endogenous LOX activity and LOX-catalyzed lipid oxidation can be regulated during meat storage and meat product manufacturing.

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Section: Resultsmentioning

confidence: 99%

Recombinant Porcine 12-Lipoxygenase Catalytic Domain: Effect of Inhibitors, Selectivity of Substrates and Specificity of Oxidation Products of Linoleic Acid

Liu

et al. 2022

Foods

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“…Similar to our results, previous studies reported low in vitro toxicity in mammalian cells. Martinez et al reported a maximal non-cytotoxic concentration of 90.4 µM in LLC-MK2 cells [ 28 ]; Soto-Acosta et al, found that 100 µM of NDGA did not reduced cell viability in Vero and U937-DC SIGN cells [ 29 ]; Koob et al, reported that NDGA was cytotoxic to tendon fibroblast cells at concentrations above 100 μM [ 38 ]. Finally, the results of Merino Ramos et al showed that 35 µM of NDGA showed cytotoxicity above 80% by measuring the cellular ATP content with the Cell Titer-Glo luminescent cell viability test, while we performed an MTT assay and found cell viability of 86% at the same concentration [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…NDGA is a phenolic lignan present in the resin of L. tridentata leaves [ 26 ]. The best characterized antiviral effects of NDGA and its derivates have been against Dengue virus (DENV), Zika virus (ZIKV), Hepatitis C virus (HCV), West Nile virus (WNV), Fort Sherman virus (FSV), Influenza virus (IV), Herpes Simplex-1 virus (HSV-1), and Human Immunodeficiency virus (HIV) [ 27 , 28 , 29 , 30 , 31 , 32 ]. To our knowledge there are no reports of NDGA antiviral activity against SARS-CoV-2.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antiviral Activity of Nordihydroguaiaretic Acid against SARS-CoV-2

Villalobos-Sánchez

García-Ruiz

Camacho-Villegas

et al. 2023

Viruses

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The coronavirus infectious disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has been spreading rapidly worldwide, creating a pandemic. This article describes the evaluation of the antiviral activity of nordihydroguaiaretic acid (NDGA), a molecule found in Creosote bush (Larrea tridentata) leaves, against SARS-CoV-2 in vitro. A 35 µM concentration of NDGA was not toxic to Vero cells and exhibited a remarkable inhibitory effect on the SARS-CoV-2 cytopathic effect, viral plaque formation, RNA replication, and expression of the SARS-CoV-2 spike glycoprotein. The 50% effective concentration for NDGA was as low as 16.97 µM. Our results show that NDGA could be a promising therapeutic candidate against SARS-CoV-2.

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Nordihydroguaiaretic acid reverses the antibacterial activity of colistin against MCR-1-positive bacteria in vivo/in vitro by inhibiting MCR-1 activity and injuring the bacterial cell membrane

et al. 2022

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First report of antiviral activity of nordihydroguaiaretic acid against Fort Sherman virus (Orthobunyavirus)

Cited by 4 publications

References 47 publications

Recombinant Porcine 12-Lipoxygenase Catalytic Domain: Effect of Inhibitors, Selectivity of Substrates and Specificity of Oxidation Products of Linoleic Acid

Recombinant Porcine 12-Lipoxygenase Catalytic Domain: Effect of Inhibitors, Selectivity of Substrates and Specificity of Oxidation Products of Linoleic Acid

In Vitro Antiviral Activity of Nordihydroguaiaretic Acid against SARS-CoV-2

Nordihydroguaiaretic acid reverses the antibacterial activity of colistin against MCR-1-positive bacteria in vivo/in vitro by inhibiting MCR-1 activity and injuring the bacterial cell membrane

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