First Report of Bacterial Leaf Streak Caused by Xanthomonas translucens pv. undulosa on Intermediate Wheatgrass (Thinopyrum intermedium) in Minnesota

Curland, Rebecca D.; Saad, Yasmeen S; Ledman, Kristi E; Ishimaru, Carol A.; Dill‐Macky, Ruth

doi:10.1094/pdis-04-19-0797-pdn

Cited by 5 publications

(1 citation statement)

References 3 publications

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“…Xanthomonas translucens is the most important bacterial seedborne disease of small grain cereals, i.e., wheat (Triticum aestivum) and barley (Hordeum vulgare) around the world (Curland et al 2018(Curland et al , 2020Khojasteh et al 2019;Langlois et al 2017).Three distinct pathovars were identified within the X. translucens species as the causal agents of BLS based on the host range of the pathogen on different small grain cereals and wild gramineous weed species. X. translucens pv.…”

Section: Bacterial Leaf Streak (Bls) Caused By Different Pathovars Ofmentioning

confidence: 99%

Transcription Activator-Like Effectors Diversity in Iranian Strains of Xanthomonas translucens

et al. 2020

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Bacterial leaf streak caused by different pathovars of Xanthomonas translucens is the most important seedborne bacterial disease of small grain cereals. However, variations in the virulence-associated genomic areas of the pathogen remain uninvestigated. In this study, the diversity of transcription activator-like effectors (TALE) was investigated using the Southern blotting of BamHI-digested genomic DNAs in the Iranian strains of X. translucens. All 65 X. translucens strains were assigned into 13 genotypes, where 57 X. translucens pv. undulosa strains were placed in genotypes 1 to 8, and seven X. translucens pv. translucens strains were placed in genotypes 9 to 12. Interestingly, we did not find any TALE genes in the strain XtKm7 (genotype 13), which showed to be pathogenic only on barley. Virulence and aggressiveness of these strains in greenhouse conditions were in agreement with the TALE-based clustering of the strains in the pathovar level, though variations were observed in the aggressiveness of X. translucens pv. undulosa strains. In general, strains containing higher numbers of putative TALE genes were more virulent on wheat and barley than strains containing fewer. This is the first TALE-based genetic diversity analysis on X. translucens strains and provides novel insights into the virulence repertories and genomic characteristics of the pathogen. Further investigations using TALE mutagenesis and complementation analysis are warranted to precisely elucidate the role of each detected X. translucens TALE in bacterial virulence and aggressiveness either on wheat or barley.

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Section: Bacterial Leaf Streak (Bls) Caused By Different Pathovars Ofmentioning

confidence: 99%

Transcription Activator-Like Effectors Diversity in Iranian Strains of Xanthomonas translucens

et al. 2020

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Xanthomonas translucens pv. undulosa (bacterial leaf streak of wheat and barley)

Osdaghi¹

2020

CABI Compendium

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Development of Genome-Driven, Lifestyle-Informed Markers for Identification of the Cereal-Infecting Pathogens Xanthomonas translucens Pathovars undulosa and translucens

et al. 2023

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Bacterial leaf streak, bacterial blight and black chaff caused by Xanthomonas translucens pathovars are major diseases affecting small grains. Xanthomonas translucens pv. translucens and X. translucens pv. undulosa are seedborne pathogens that cause similar symptoms on barley, but only X. translucens pv. undulosa causes bacterial leaf streak of wheat. Recent outbreaks of X. translucens have been a concern for wheat and barley growers in the Northern Great Plains; however, there are limited diagnostic tools for pathovar differentiation. We developed a multiplex PCR based on whole-genome differences to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. We validated the primers across different Xanthomonas and non-Xanthomonas strains. To our knowledge, these are the first multiplex PCR to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. These molecular tools will support disease management strategies enabling detection and pathovar incidence analysis of X. translucens.

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First Report of Bacterial Leaf Streak Caused by Xanthomonas translucens pv. undulosa on Intermediate Wheatgrass (Thinopyrum intermedium) in Minnesota

Cited by 5 publications

References 3 publications

Transcription Activator-Like Effectors Diversity in Iranian Strains of Xanthomonas translucens

Transcription Activator-Like Effectors Diversity in Iranian Strains of Xanthomonas translucens

Xanthomonas translucens pv. undulosa (bacterial leaf streak of wheat and barley)

Development of Genome-Driven, Lifestyle-Informed Markers for Identification of the Cereal-Infecting Pathogens Xanthomonas translucens Pathovars undulosa and translucens

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