First report of blaNDM-1-producing Acinetobacter baumannii isolated in Lebanon from civilians wounded during the Syrian war

Rafei, Rayane; Dabboussi, Fouad; Hamzé, Monzer; Eveillard, Matthieu; Lemarié, Carole; Mallat, Hassan; Rolain, Jean‐Marc; Joly-Guillou, Marie-Laure; Kempf, Marie

doi:10.1016/j.ijid.2014.01.004

Cited by 76 publications

(60 citation statements)

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“…We observed good correlation between MLST and bla OXA-51 typing, since each bla OXA-51 like gene variant had its specific ST, with two exceptions: ST216 and ST470, which had the same bla OXA-51 gene, and ST295 and ST468, which had the same bla OXA-344 gene. Our bla OXA-51 gene-sequencing results were concordant with the worldwide MLST results reported in other studies, such as bla OXA-69 usually being associated with ST1 (51), bla OXA-120 with ST193 (52), bla OXA-68 with ST10 (3,53), and bla OXA-94 with ST85 (15). Finally, some bla OXA-51 variants found in our new STs have previously been described in human STs.…”

Section: Discussionsupporting

confidence: 91%

“…In Lebanon, only strains belonging to a clinical context have been studied (15,(38)(39)(40), and no data concerning the occurrence of A. baumannii outside Lebanese hospitals are available. The aim of this study was to look for the presence of A. baumannii in different environments, to study its susceptibility to antibiotics, and to characterize the predominant community genotypes by using two genotyping approaches: multilocus sequence typing (MLST) and bla OXA-51 sequence-based typing (SBT).…”

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confidence: 99%

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Extrahuman Epidemiology of Acinetobacter baumannii in Lebanon

Rafei

Hamzé

Pailhoriès

et al. 2015

Appl Environ Microbiol

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The presence of Acinetobacter baumannii outside hospitals is still a controversial issue. The objective of our study was to explore the extrahospital epidemiology of A. baumannii in Lebanon. From February 2012 to October 2013, a total of 73 water samples, 51 soil samples, 37 raw cow milk samples, 50 cow meat samples, 7 raw cheese samples, and 379 animal samples were analyzed by cultural methods for the presence of A. baumannii. Species identification was performed by rpoB gene sequencing. Antibiotic susceptibility was investigated, and the A. baumannii population was studied by two genotyping approaches: multilocus sequence typing (MLST) and bla OXA-51 sequence-based typing (SBT). A. baumannii was detected in 6.9% of water samples, 2.7% of milk samples, 8.0% of meat samples, 14.3% of cheese samples, and 7.7% of animal samples. All isolates showed a susceptible phenotype against most of the antibiotics tested and lacked carbapenemase-encoding genes, except one that harbored a bla OXA-143 gene. MLST analysis revealed the presence of 36 sequence types (STs), among which 24 were novel STs reported for the first time in this study. bla OXA-51 SBT showed the presence of 34 variants, among which 21 were novel and all were isolated from animal origins. Finally, 30 isolates had new partial rpoB sequences and were considered putative new Acinetobacter species. In conclusion, animals can be a potential reservoir for A. baumannii and the dissemination of new emerging carbapenemases. The roles of the novel animal clones identified in community-acquired infections should be investigated.

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

Extrahuman Epidemiology of Acinetobacter baumannii in Lebanon

Rafei

Hamzé

Pailhoriès

et al. 2015

Appl Environ Microbiol

Self Cite

113

101

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“…Of interest, our bla NDM-1 -carrying isolates belonged to ST85, an infrequent ST worldwide, which has recently been detected in A. baumannii carrying the bla NDM-1 gene in Lebanon and in France Decousser et al, 2013;Rafei et al, 2014). In France, in particular, the first outbreak of NDM-1-producing A. baumanni, whose index case was a patient transferred from Algeria, has been described (Decousser et al, 2013).…”

Section: Discussionmentioning

confidence: 79%

“…To the best of our knowledge, our study presents the first report of NDM-1-producing A. baumannii in Turkey. NDM-1-producing A. baumannii have been previously identified in countries such as France, Algeria, Kenya, Syria and China (Chen et al, 2011;Boulanger et al, 2012;Bonnin et al, 2013;Decousser et al, 2013;Revathi et al, 2013;Rafei et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

NDM-1-producing Acinetobacter baumannii ST85 now in Turkey, including one isolate from a Syrian refugee

Heydari

Mammina

Köksal

2015

Journal of Medical Microbiology

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New Delhi metallo-b-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat owing to the extended hydrolysis of b-lactams including carbapenems. Here, to the best of our knowledge we describe for the first time in Turkey two NDM-1-producing Acinetobacter baumannii isolates recovered from intensive care unit patients. The presence of bla NDM-1 was detected by PCR and confirmed by sequencing. The clonal relationship was assessed by PFGE and multilocus sequence typing. Both isolates were positive for bla NDM-1 and were attributed with the sequence type 85. One isolate was from a Syrian refugee, whereas the second was from a patient who had never travelled outside Turkey. Our findings confirmed that the rapid spread of NDM-1-producing Gram-negative organisms could become a major challenge for the treatment and control of healthcare-associated infections in our geographical area. They suggest also that NDM-1-producing strains and/or their genetic determinants are probably being imported from Syria to neighbouring countries.

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“…This is in agreement with previous work reported on CRAB in the Gulf region (3). However, genes of the NDM type carried by CRAB have been reported from the Indian subcontinent (39,40), Asia (41), Lebanon (42), and Europe (5). A related outbreak with five cases was reported from France in an intensive care unit (ICU) where the index patient was transferred from Algeria (43).…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolates in the Gulf Cooperation Council States: Dominance of OXA-23-Type Producers

et al. 2015

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(1). The success of this pathogen is partially due to the high prevalence of a multidrug-resistant phenotype that A. baumannii now demonstrates (2). In the Middle East, particularly in states of the Cooperation Council for the Arab States of the Gulf (Gulf Cooperation Council [GCC]; i.e., Saudi Arabia, United Arab Emirates, Oman, Kuwait, Qatar, and Bahrain), the prevalence of carbapenem-resistant A. baumannii (CRAB) has increased dramatically over the last decade (3). This high prevalence limits treatment options, which can lead to increased morbidity and mortality due to infections caused by CRAB.The phenotypic resistance characteristics of CRAB are mainly due to the expression of class D carbapenemases, called oxacillinases. Moreover, plasmid-mediated metallo-␤-lactamases (MBL) have been associated with the resistance phenotype (2). The existence of ISAba1 elements upstream of the bla OXA-51-type gene is also associated with the carbapenem resistance phenotype in A. baumannii by overexpressing the intrinsic OXA-51 carbapenemase (4). Previous reports on isolates from the GCC states show that the carbapenem resistance phenotype in A. baumannii is often due to the expression of OXA enzymes, particularly OXA-23 (3). However, MBL-encoding genes, including the recently

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First report of blaNDM-1-producing Acinetobacter baumannii isolated in Lebanon from civilians wounded during the Syrian war

Abstract: These findings show that Syria constitutes a reservoir for NDM-1-producing bacteria. These results also highlight the need for effective measures to stop the threatening spread of such strains.

Cited by 76 publications

References 10 publications

Extrahuman Epidemiology of Acinetobacter baumannii in Lebanon

Extrahuman Epidemiology of Acinetobacter baumannii in Lebanon

NDM-1-producing Acinetobacter baumannii ST85 now in Turkey, including one isolate from a Syrian refugee

Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolates in the Gulf Cooperation Council States: Dominance of OXA-23-Type Producers

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