First Report of Bougainvillea spectabilis chlorotic vein-banding virus Infecting Bougainvillea Plants in Taiwan

Tsai, Ching Hwa; Su, Hong‐Ji; Liao, Yuan-Yin; Hung, Ting‐Hsuan

doi:10.1094/pd-89-1363c

Cited by 10 publications

(12 citation statements)

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“…The same authors also generated a partial sequence (465 bp) of the viral DNA. Simultaneously, another badnavirus from Taiwan was also reported to cause mottling, chlorosis and vein banding in bougainvillea; the partial (676 bp) sequence of the latter shared 82% identity to the former (Tsai et al, 2005). Two new isolates of BsCVBV were partially characterized from southern and northern India (Baranwal et al, 2010).…”

Section: Diversity Distribution and Host Rangementioning

confidence: 98%

Bacilliform DNA‐containing plant viruses in the tropics: commonalities within a genetically diverse group

Borah

Sharma

Kant

et al. 2013

Molecular Plant Pathology

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Section: Diversity Distribution and Host Rangementioning

confidence: 98%

Bacilliform DNA‐containing plant viruses in the tropics: commonalities within a genetically diverse group

Borah

Sharma

Kant

et al. 2013

Molecular Plant Pathology

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“…Bougainvillea chlorotic vein-banding disease caused by Bougainvillea spectabilis chlorotic vein-banding virus (BsCVBV) was first reported in Brazil in 2001 and subsequently from Taiwan and India [ 106 , 107 , 108 ]. The diseased bougainvillea plants developed symptoms such as mottling, chlorosis, vein-banding, leaf distortion, and stunting.…”

Section: Virus Characterizationmentioning

confidence: 99%

Badnaviruses: The Current Global Scenario

Bhat

Höhn²,

Selvarajan

2016

Viruses

154

105

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Badnaviruses (Family: Caulimoviridae; Genus: Badnavirus) are non-enveloped bacilliform DNA viruses with a monopartite genome containing about 7.2 to 9.2 kb of dsDNA with three to seven open reading frames. They are transmitted by mealybugs and a few species by aphids in a semi-persistent manner. They are one of the most important plant virus groups and have emerged as serious pathogens affecting the cultivation of several horticultural crops in the tropics, especially banana, black pepper, cocoa, citrus, sugarcane, taro, and yam. Some badnaviruses are also known as endogenous viruses integrated into their host genomes and a few such endogenous viruses can be awakened, e.g., through abiotic stress, giving rise to infective episomal forms. The presence of endogenous badnaviruses poses a new challenge for the fool-proof diagnosis, taxonomy, and management of the diseases. The present review aims to highlight emerging disease problems, virus characteristics, transmission, and diagnosis of badnaviruses.

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“…2). Previously, bacilliform virus particles have been shown to be associated with bougainvillea plants with CVB in Brazil (Rivas et al 2005) and mottling, chlorosis, vein-banding and stunting in Taiwan (Tsai et al 2005).…”

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confidence: 99%

First report of two distinct badnaviruses associated with Bougainvillea spectabilis in India

2010

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Two distinct types of symptoms, severe yellow mosaic and chlorotic vein-banding, were observed on Bougainvillea spectabilis at two different locations in India. Electron microscopy revealed bacilliform virus particles in leaf samples from both places. PCR amplification, cloning, sequencing and sequence analysis of reverse transcriptase and ribonuclease H coding region in open reading frame 3 indicated that two distinct badnaviruses are associated with B. spectabilis in India.Keywords Badnavirus Á Bougainvillea spectabilis Á Delhi Á Tirupati Á PCR Bougainvillea spectabilis Willd (flower paper) is a widely grown ornamental plant in India. During 2008, B. spectabilis plants developed distinct severe yellow mosaic (SYM) in parts of Tirupati (Fig. 1a). Subsequently in 2009, a chlorotic vein-banding (CVB) was noticed on leaves of B. spectabilis on the campus of the Indian Agricultural Research Institute, New Delhi (Fig. 1b). Leaf dip electron microscopy of symptomatic bougainvillea leaves revealed the association of bacilliform virus particles measuring 120-150 nm 9 20 nm (Fig. 2). Previously, bacilliform virus particles have been shown to be associated with bougainvillea plants with CVB in Brazil (Rivas et al. 2005) and mottling, chlorosis, vein-banding and stunting in Taiwan (Tsai et al. 2005).To confirm the virus identity, symptomatic leaves of bougainvillea collected from Tirupati and Delhi were subjected to polymerase chain reaction (PCR) amplification using two degenerate Badnavirus primers viz. BadnaFP (5 0 ATGCCITTYGGIAARAAYGCICC3 0 ) and BadnaRP (5 0 CCAYTTRCAIACISCICCCCAICC3 0 ) corresponding to putative ribonuclease H (RNase H) and reverse transcriptase (RT) coding regions (Yang et al. 2003). Both samples yielded an amplicon of approximately 600 bp, and no amplification was observed in healthy, symptomless bougainvillea (Fig. 3). The amplicons were cloned in pGEM-T easy vector (Promega, Madison, WI, USA) and sequenced. BLAST analysis of the 577 bp of RNase H and RT nucleotide sequences of viral DNA from bougainvillea with SYM symptoms from Tirupati (accession GQ254410) had maximum sequence identity of 73% in open reading frame 3 (ORF 3) of Bougainvillea spectabilis chlorotic vein-banding virus (BsCVBV) (accessions EU034539, DQ347841, and DQ103759). An identical sequence size of amplified viral DNA isolated from bougainvillea with CVB symptoms from Delhi (accession GQ254411) had 72% sequence identity with the sequences of same three BsCVBV isolates. Nucleotide and amino acid sequences of putative RT and RNase H region of ORF 3 of these two badnaviruses were analyzed using Bio Edit version 7.0.4 for sequence identities with other badnaviruses. The two badnaviruses infecting bougainvillea in India shared an identity of 76.2% in nucleotide and 88.6% in amino acid sequences with each other. Comparison of nucleotide and amino acid sequences of badnaviruses infecting bougainvillea from India and Taiwan showed identities in the range of 72.3-73.5% for nucleotides and 83.4-84.9% in amino acids of RT and RNase H. Be...

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First Report of Bougainvillea spectabilis chlorotic vein-banding virus Infecting Bougainvillea Plants in Taiwan

Cited by 10 publications

References 0 publications

Bacilliform DNA‐containing plant viruses in the tropics: commonalities within a genetically diverse group

Bacilliform DNA‐containing plant viruses in the tropics: commonalities within a genetically diverse group

Badnaviruses: The Current Global Scenario

First report of two distinct badnaviruses associated with Bougainvillea spectabilis in India

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