First Report of Chromosome Analysis of Two Chaetodontid Fishes (Perciformes, Chaetodontidae)

Abstract

We report the first chromosome analysis in the Indian vagabond butterflyfish (Chaetodon decussatus) and lined butterflyfish (C. lineolatus) from Andaman Sea, Phuket Province, Thailand. Kidney cell samples were taken from four male and four female fishes. The mitotic chromosome preparation was directly prepared from kidney cells. The chromosomes were stained with conventional and Ag-NOR staining techniques. The results showed that the diploid chromosome number of C. decussatus and C. lineolatus was 2n=48, and the fundamental number (NF) was 48 in both males and females. The chromosomes were present as large telocentric and medium telocentric chromosomes in numbers of 24-24 and 22-26, respectively. There was no observation of strange size chromosomes related to sex. After Ag-NOR banding technique, a single pair of nucleolar organizer regions (NORs) was observed on the long arm centromeric region of medium telocentric chromosome pair 18 in C. decussatus and on the long arm subcentromeric region of medium telocentric chromosome pair 17 in C. lineolatus. The karyotype formulas could be deduced as:

Karyotypic stasis and its implications for extensive hybridization events in corallivores species of butterflyfishes (Chaetodontidae)

“… L = large chromosome; M = medium chromosome; S = small chromosome. References: [ 60 ] Arai and Yamamoto, 1981 [ 61 ]; Galetti et al (2006) [ 57 ]; Arai and Inoue (1975) [ 62 ]; Supiwong et al (2017a) [ 64 ]; Supiwong et al (2017b) [ 63 ]; Na Nongkhai (2014) [ 65 ]; Molina et al (2013) [ 66 ]; Affonso et al (2001) [ 67 ]; Nagpure et al (2006) [ 68 ]; Boonsuk (2013) [ 69 ]; Ojima and Yamamoto (1990) [ 70 ]; Jumrusthanasan and Supiwong (2015) [ 54 ]; Supiwong et al (2015) [ 71 ]; Sonsrin and Pinthong, 2018. …”

The First Chromosomal Characteristics of Nucleolar Organizer Regions and Karyological Analysis of Two Chaetodontid Fishes (Perciformes, Chaetodontidae)

“…All specimens were maintained in aerated, flowing seawater aquaria until analysis. Mitotic chromosome preparations were made from anterior kidney after in vivo treatment using a method modified from Chen and Ebeling (1968), Gold et al (1990), Kasiroek et al (2017) and Supiwong et al (2017). The chromosomes were stained with 10% Giemsa s for 30 min (Chooseangjaew et al 2017) and identified for NORs by Ag-NOR staining technique (Howell andBlack 1980, Sangpakdee et al 2017).…”

“…In the present work, cytogenetical analyses by conventional staining and Ag-NOR banding techniques were carried out on two wild species from the Andaman Sea, Thailand including C. triangulum and C. andamanensis. The study would also improve our understanding of karyotype evolution mechanism and speciation in the family Chaetodontidae (Supiwong et al 2017), and increase knowledge available for implementation of polyploidy manipulation, hybridization, sex control and other genetic improvements in the future.…”

History and New Developments on the 90th Anniversary of Cytologia: On the Occasion of the Publication of “Dawn of Modern Wheat Genetics”

“…In Thailand, including Indochina and the northern Malay peninsula, Alongklod Tanomtong and coworkers have recently focused on fish karyotype analysis and have published 15 reports in Cytologia over the last 2 years on the following fish species: Humpback cardinalfish, Fibramia lateralis (Kasiroek et al 2017a); Banggai cardinalfish, Pterapogon kauderni (Kasiroek et al 2017b); triangle butterflyfish, Chaetodon triangulum and yellow butterflyfish, C. andamanensis (Supiwong et al 2017a); Indian vagabond butterflyfish, Chaetodon decussatus and Lined butterflyfish, C. lineolatus (Supiwong et al 2017b); Nile tilapia, Oreochromis niloticus (Sriuttha et al 2017); three-spot damselfish, Dascyllus trimaculatus (Getlekha et al 2017); ocellated dragonet, Synchiropus ocellatus and picturesque dragonet, S. picturatus (Kasiroek et al 2017c); crystal eye catfish, Hemibagrus wyckii (Supiwong et al 2017d); Chevey s sheetfish, Micronema cheveyi (Pinthong et al 2017); F 1 hybrid catfish: Mekong giant catfish, Pangasianodon gigas striped catfish, P. hypophthalmus and spot pangasius, Pangasius larnaudii Pangasianodon hypophthalmus (Sreeputhorn et al 2017); whitecheek monocle bream, Scolopsis vosmeri (Supiwong et al 2017c); snakehead fishes, Channa marulius and C. marulioides (Sarasan et al 2018); Hihgfin barb fish, Cyclocheilichthys armatus (Chaiyasan et al 2018); black lancer, Bagrichthys majusculus (Supiwong et al 2018); and longfin carp, Labiobarbus leptocheilus (Saenjundaeng et al 2018).…”