First report of Cryptosporidium deer-like genotype in Malaysian cattle

Halim, N. Abdul; Plutzer, Judit; Bakheit, Mohammed A.; Karanis, Panagiotis

doi:10.1016/j.vetpar.2007.12.035

Cited by 30 publications

(18 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other Cryptosporidium species have been reported to infect cattle including C. bovis, C. andersoni, and the Cryptosporidium deer-like genotype (Santín et al 2004;Fayer et al 2006;Abdul Halim et al 2008), but none of these were found here in our few PCR positive samples. The most common zoonotic species is C. parvum and it is also the most common species infecting cattle.…”

Section: Discussioncontrasting

confidence: 61%

Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand

et al. 2008

View full text Add to dashboard Cite

This study was performed to determine the prevalence and genotypes of Cryptosporidium species among HIV patients and cattle in Thailand. Stool specimens were collected from 46 HIV patients from Prabat Nampu Temple, Lop Buri Province in central Thailand. Two hundred fecal samples from dairy cattle were collected from seven farms in Chon Buri Province, the eastern part of Thailand. Each sample was concentrated by Sheather's sucrose flotation technique and stained by acid fast stain (AFS) for the identification of oocysts by microscopy. All HIV stool samples and 83 fecal specimens from cattle were further tested using nested polymerase chain reaction (PCR) targeting the 18S SSUrRNA gene to characterize the detected species. In HIV patient samples, the detection rate was 28.7% by AFS and 4.35% by nested PCR. In cattle samples, the detection rate was 13% by AFS and 9.63% by nested PCR. After DNA sequencing results, we identified the genotypes of the Cryptosporidium from seven of the PCR positive samples. All were found to be C. parvum. The findings presented here represent the first genetic identification of Cryptosporidium species in cattle in Thailand.

show abstract

Section: Discussioncontrasting

confidence: 61%

Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand

et al. 2008

View full text Add to dashboard Cite

show abstract

“…In the present study, Cryptosporidium infection and species were determined using a molecular method for pre-weaned calves in Shaanxi Province, north-western Species determination is essential for evaluation of the zoonotic source of cryptosporidiosis and control of this disease. Four Cryptosporidium species, namely C. bovis, C. andersoni, C. parvum and C. ryanae, have been identified as the most common species in cattle in different countries, including China (Geurden et al, 2006(Geurden et al, , 2007Starkey et al, 2006;Coklin et al, 2007;Feng et al, 2007;Langkjaer et al, 2007;Plutzer & Karanis, 2007;Thomaz et al, 2007;Thompson et al, 2007;Halim et al, 2008;Szonyi et al, 2008;Brook et al, 2009;Chen & Huang, 2012), with C. andersoni reported as the most frequent species in yearlings and adult cattle. C. ryanae and C. bovis have been identified as the dominant species in cattle older than 6 months, and C. parvum as the species most common in pre-weaned dairy calves in the USA (Fayer et al, 2006Santín et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China

Fang

Wang

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

“…Since then, there have been numerous reports of cryptosporidiosis in different countries and regions [5,6,17,19,20,27]. However, only a few publications have documented Cryptosporidium prevalence among animals in China [7,40].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization ofCryptosporidiumspp. in dairy cattle from farms in China

Chen

Huang

2012

J Vet Sci

View full text Add to dashboard Cite

Fecal samples of 2,056 dairy cattle from 14 farms were collected in three geographical regions of China and stained using a modified acid-fast staining technique to identify Cryptosporidium oocysts. A total of 387 (18.82%) positive samples were identified and further analyzed by polymerase chain reaction (PCR) using primers designed to amplify DNA fragments from the small subunit ribosomal RNA. The PCR products were sequenced and the sequences were deposited in the GenBank database under accession numbers EU369377-84 and GU070730-33. Phylogenetic analysis was performed and a distances matrix generated from these sequences confirmed the existence of Cryptosporidium ( C. ) parvum 'mouse' genotype, C. bovis , C. andersoni , C. hominis , and C. serpentis in cattle. These results represent the first report on the prevalence and genetic identification of Cryptosporidium species, and may contribute to a better understanding of the epidemiology of Cryptosporidium in cattle in China.

show abstract

First report of Cryptosporidium deer-like genotype in Malaysian cattle

Cited by 30 publications

References 30 publications

Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand

Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand

Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China

Prevalence and molecular characterization ofCryptosporidiumspp. in dairy cattle from farms in China

Contact Info

Product

Resources

About