First report of cucumber mosaic virus infecting Alliaria petiolata in Ukraine

Kyrychenko, A. M.; Snihur, Halyna; Shevchenko, T.

doi:10.1007/s42161-022-01107-0

Cited by 2 publications

(3 citation statements)

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“…3) CMV is widespread in central Ukraine (Kyiv region) with 80% incidence among symptomatic plants surveyed in the study. This is consistent with our previous result on the detection of CMV in A. petiolata in Ukraine (Kyrychenko et al 2022). It is noteworthy that 20% of CMV-positive samples were co-infected with TuMV.…”

Section: Discussionsupporting

confidence: 93%

“…Besides a large number of horticultural crops, CMV infects many weed species. In Ukraine, CMV has been detected on a various host plants, and as our previous study showed, the virus can infect naturally garlic mustard as well (Kyrychenko et al 2022). The natural occurrence of TuMV infection in A. petiolata has been confirmed in Europe (Horvath et al 1975, Lisa andLovisolo 1976) and Canada (Stobbs and Van Schagen 1987) since 1975.…”

Section: Discussionmentioning

confidence: 83%

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Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine

Kyrychenko¹,

Snihur²,

Shevchenko³

et al. 2023

Biol. plant.

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Garlic mustard (Alliaria petiolata) is an herbaceous biennial plant native to Europe. In Ukraine, in addition to becoming a serious invader, garlic mustard can serve as a host to several viruses, which may affect agricultural crops. In view of this, the purpose of the study was to identify the virome of garlic mustard growing in Ukraine. Plant samples collected in Kyiv regions were tested for the presence of cucumber mosaic virus (CMV), turnip mosaic virus (TuMV), turnip yellow mosaic virus (TYMV), watermelon mosaic virus II (WMV), and turnip crinkle virus (TCV) by serological and/or molecular methods. According to the results found in the present study, symptomatic A. petiolata obtained in 2021 were infected with CMV (60%), TuMV (20%), or co-infected with CMV + TuMV (20%). TYMV, WMV II, and TCV were not detected in any of the collected samples. The cDNA fragments encoded the coat protein (CP) gene of CMV and TuMV were sequenced and named as CMV-Ap and TuMV-Ap, respectively. In phylogenetic analysis, the CMV-Ap closely resembled the German isolate MW582807 (Sarracenia sp.), with 99.8% nucleotide identity and belongs to subgroup II of CMV. In the phylogenetic tree, TuMV-Ap clustered with isolates AP017803, AP017764, AP017791, and JQ073722, and represented the highest identity (98.6%) to Iranian isolate IRNTRa9 (AP017803) from Rapistrum rugosum and Turkish isolate TUR49 (AP017872) from Raphanus raphanistrum. The sequences of CMV-Ap and TuMV-Ap were deposited in the GenBank under Accession Numbers MZ540213 and OM799323, respectively. The results obtained in the study indicate the important role of infected garlic mustard as alternative host and natural reservoir of CMV and TuMV from which these economically important viruses can spread to other wild and cultivated plants. This is the first molecular evidence of TuMV infection in A. petiolata from Ukraine.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 83%

Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine

Kyrychenko¹,

Snihur²,

Shevchenko³

et al. 2023

Biol. plant.

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“…This may be due to the fact that, unlike ZYMV and WMV-2, CMV can be transmitted not only by aphids but also by dodder and seed. To date, it has been confirmed that various species of wild flora may be the source of CMV in Ukraine [17], serving as additional risk factor for agricultural plants. In addition, there is evidence of CMV interaction with members of Potyvirus family.…”

Section: Fig 2 Mixed Infections Found In Solanaceous (Cmv+tomvandcmv+...mentioning

confidence: 94%

Mixed Viral Infections in Vegetables in Ukraine

Shevchenko¹,

Bederak²,

Taher³

et al. 2022

BTSNUKSB

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Aim. Screening of vegetable crops for mixed viral infections caused by 8 viruses, and evaluation of relative share for different combinations of pathogens using serological methods for plant virus diagnostics. Methods. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was performed for detection of viral antigens. Results. For this study, the samples from plants with virus-like symptoms were collected from the agroecosystems in 4 regions of Ukraine during three vegetative seasons in 2019-2021. Plant samples were tested for presence of the following viruses: cucumber mosaic virus (CMV), watermelon mosaiс virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), tomato mosaic virus (ToMV), tobacco rattle virus (TRV), potato virus Y (PVY), potato virus X (PVX), and tomato spotted wilt virus (TSWV). Among the screened plants with virus-like symptoms, three viruses were the most common: CMV, WMV2 and ZYMV, with the incidence rate of 32%, 33% and 29%, respectively. Apart from monoinfected plants, several patterns of mixed infections were shown typically induced by two and less commonly by three viruses (CMV+WMV2+ZYMV). From these patterns of mixed infections, five groups of pathogens were registered. Conclusions. In this work, we have analyzed cucurbit and solanaceous vegetable crops with virus-like symptoms for viral infections caused by 8 pathogens, and established virus combinations inducing mixed infections in the field. Five groups of pathogens were demonstrated as such combinations: CMV+ToMV, CMV+PVY, CMV+WMV2, CMV+ZYMV, and WMV2+ZYMV, with CMV+ToMV being the most common for tested plants. Also, there was one group of pathogens formed by three viruses CMV+WMV2+ZYMV. CMV has been shown present in every group of mixed viral infections in plants from both families, Cucurbitaceae and Solanaceae. The incidence rate for these combinations differed from 4 to 8%. The presented results are important in the context of ecology and epidemiology of viral diseases of vegetables.

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First report of cucumber mosaic virus infecting Alliaria petiolata in Ukraine

Cited by 2 publications

References 1 publication

Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine

Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine

Mixed Viral Infections in Vegetables in Ukraine

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