First report of Cymbidium mosaic virus and Odontoglossum ring spot virus in Argentina

Abstract

Oncidium Sharry Baby orchids from nursery greenhouses in Buenos Aires, Argentina with viral symptoms were processed for transmission electron microscope observations. Two types of viral particles, morphologically different, were observed. The aim of this work is identification of viral particles infecting the orchids. Serological analysis and partial genome sequence confirmed the presence of Cymbidium mosaic virus (CymMV) and Odontoglossum ring spot virus (ORSV). To our knowledge this is the first report of ORSV and CymMV in Argentina.

Cymbidium mosaic virus y Odontoglossum ringspot virus en diversos generos de orquideas cultivadas en Mexico

“…Las plantas indicadoras más usadas para diagnosticar al CymMV son N. benthamiana, Cucumis sativus, D. stramonium, Gomphrena globosa on the results of this study, it is also possible to use N. glutinosa and N. occidentalis. It has been corroborated that C. quinoa and C. amaranticolor are better hosts for ORSV and Datura stramonium for CymMV, although there is no selectivity (Cánovas et al, 2016). However, this investigation found differences in the symptoms caused by CymMV and ORSV in D. stramonium, since the first caused smaller chlorotic lesions.…”

“…Revista Mexicana de FITOPATOLOGÍA Mexican Journal of Phytopathology y C. amarticolor (Brunt et al, 1996) y, con base en los resultados del presente estudio, es posible utilizar también a N. glutinosa y N. occidentalis. Se ha corroborado que C. quinoa y C. amaranticolor son mejores hospedantes para ORSV y Datura stramonium para CymMV, aunque no hay selectividad (Cánovas et al, 2016); sin embargo, en esta investigación se observó diferencia en los síntomas ocasionados por CymMV y ORSV en D. stramonium, ya que el primero provocó lesiones cloróticas anilladas mientras que el segundo ocasionó lesiones cloróticas de menor tamaño. El aislamiento de CymMV ocasionó infección sistémica en N. benthamiana y se sabe que existen aislamientos de este virus que son capaces de infectar o no sistémicamente a esta planta (Hsiang-Chia et al, 2009).…”

Annual Accumulation of CymMV May Lead to Loss in Production of Asymptomatic Vanilla Propagated by Cuttings

“…Among them, Cymbidium mosaic virus (CymMV) belonging to the family Alfaflexiviridae is one of the most widespread virus and is known to infect Vanilla planifolia and Vanilla tahitensis in French Polynesia and Reunion Island [ 8 ]. CymMV infection in orchids can be detected from the symptoms such as mosaic patterns, flowers with deformities and colour breaking, sunken chlorotic or necrotic patches on leaves and stems [ 10 , 11 , 12 ].…”

Genetic homogenisation of two major orchid viruses through global trade‐based dispersal of their hosts

“…ORSV infection can result in flower malformation and color breaking, and ringspots on the leaves. As with CymMV, the transmission of ORSV appears to be by means of mechanical inoculation (Cánovas, Ballari, & Nome, ; Hu et al, ; Zettler et al, ).…”