First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe

Loddo, Donato; Imperatore, Gaetano; Milani, Andrea; Panozzo, Silvia; Farinati, Silvia; Sattin, M.; Zanin, Giuseppe

doi:10.3390/agronomy10111692

Cited by 6 publications

(7 citation statements)

References 41 publications

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“…Currently, 296 weed species are herbicideresistant, with glyphosate and paraquat-resistant weed species totaling 51 and 31, including goosegrass (Heap 2023). Resistance of goosegrass found in this study must be carefully addressed and managed because areas with good weed management can be susceptible to resistant weeds due to the spread of resistant weeds from the surrounding land area; because goosegrass is a self-pollinating species, the dissemination of glyphosate-resistant biotypes is modest via pollen movement (Loddo et al 2020). Glyphosate-resistant biotypes can spread by seed movement via machinery, plant materials, or substrates (Loddo et al 2020).…”

Section: Discussionmentioning

confidence: 96%

“…Weeds can become resistant due to repeated herbicide applications without herbicide rotation over a long period, even if the correct dosage is used. In the Italian region of Caserta, weeds in crop nurseries were controlled by making 3 to 4 applications of glyphosate per year at the recommended dose, but this still led to the emergence of glyphosateresistant goosegrass weeds (Loddo et al 2020). However, due to the absence of herbicide rotation, goosegrass has adapted and become resistant to glyphosate herbicides (Loddo et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…In the Italian region of Caserta, weeds in crop nurseries were controlled by making 3 to 4 applications of glyphosate per year at the recommended dose, but this still led to the emergence of glyphosateresistant goosegrass weeds (Loddo et al 2020). However, due to the absence of herbicide rotation, goosegrass has adapted and become resistant to glyphosate herbicides (Loddo et al 2020). According to reports from Malaysia, goosegrass is also resistant to paraquat, glufosinate, and glyphosate.…”

Section: Discussionmentioning

confidence: 99%

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Resistance of Eleusine indica to non-selective herbicides in Indonesian oil palm plantation

UMIYATI,

KURNIADIE,

WIDIANTO

et al. 2023

Biodiversitas

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Abstract. Umiyati U, Kurniadie D, Widianto R, Widayat D, Nasahi C. 2023. Resistance of Eleusine indica to non-selective herbicides in Indonesian oil palm plantation. Biodiversitas 24: 4661-4667. Indonesia is the world's largest producer of oil palm. One biological constraint that may reduce the national oil palm production is Eleusine indica (L.) Gaertn (goosegrass). This weed is usually controlled chemically using non-selective herbicides, such as glyphosate, paraquat, and ammonium glufosinate. Although these herbicides are widely used, there are minimal case reports of weed resistance in Indonesia. This study aimed to map and quantify the herbicide resistance of goosegrass in Indonesian oil palm plantations. Weed mortality after herbicide application was used to determine the resistance. The study collected samples from Indonesia's six primary oil palm regions: West Sumatra, North Sumatra, Riau, Lampung, Belitung, and West Kalimantan. The sample collection process was supplemented by conducting interviews to get information on the herbicide application practices of palm oil growers. The results showed that 15 samples of goosegrass from various locations were resistant to glyphosate, 17 were resistant to paraquat, and one showed developing resistance to ammonium glufosinate. The most resistant goosegrass was found in North Sumatra, with a resistant index of 3.32 and 3.81 to glyphosate and paraquat, respectively. At the same time, the resistance to ammonium glufosinate could not be measured because the low doses of ammonium glufosinate were sufficient to control the weeds.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Resistance of Eleusine indica to non-selective herbicides in Indonesian oil palm plantation

UMIYATI,

KURNIADIE,

WIDIANTO

et al. 2023

Biodiversitas

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“…Glyphosate-resistant (GR) goosegrass was first reported in Malaysia (Lee and Ngim 2000). Then the following reports are from Australia, Brazil, and China (Chen et al 2015;Takano et al 2017;Loddo et al 2020). GR goosegrass has been reported in oil-palm plantations in North Sumatra, Indonesia (Tampubolon et al 2019;Purba and Sipayung 2021).…”

Section: Introductionmentioning

confidence: 99%

Possibility of glyphosate-resistant goosegrass (Eleusine indica) in citrus fields in Rejang Lebong Districts, Bengkulu, Indonesia

SIMARMATA,

APRIANTONEDI,

SETYOWATI

et al. 2023

Biodiversitas

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Abstract. Simarmata M, Apriantonedi R, Setyowati N, Pujiwati H. 2023. Possibility of glyphosate-resistant goosegrass (Eleusine indica) in citrus fields in Rejang Lebong Districts, Bengkulu, Indonesia. Biodiversitas 24: 3633-3638.Weed abundances dominated by goosegrass (Eleusine indica L.) are a major constraint in the citrus fields of Rejang Lebong District, Province of Bengkulu, Indonesia. The objectives of this research were to investigate weed control methods applied by citrus farmers, analyze the dominant weed, and study the possibility of glyphosate resistance in goosegrass. A survey was conducted by interviewing three citrus farmers regarding weed control measures, followed by weed assessment in 10 sampling plots of each location. Then, field trials were conducted to evaluate glyphosate efficacy on weeds in the three locations of citrus fields. The survey found that glyphosate has been applied for weed control up to 4 L ha-1( 1.92 kg a.i. ha-1 of isopropyl amine salt) 2 to 3 times a year, and the farmers found that some weeds were resistant to glyphosate. Weed assessment showed that goosegrass is a dominant species with a summed dominance ratio (SDR) of 67.1, 70.8, and 79.0% at each location, respectively. The field trials observed that the levels of survival weeds in the experimental plots treated with glyphosate at 0.5, 1.0, 2.0, 4.0, and 8.0 L ha-1were 94-95%, 83-85.3%, 70-73.3%, 20-29%, and 10-12.3%, respectively. Goosegrass was identified as the survivor's weeds. The glyphosate doses (LD50) required to eradicate 50% of the weed populations ranged between 2 and 4 L ha-1 at the three sites. This study proved that goosegrass in the citrus groves of Rejang Lebong District, Bengkulu, is resistant to glyphosate.

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“…The Pro106Ser mutation in the EPSPS was first reported in Eleusine indica [ 12 ]. Since then, the different mutation types (Pro106His, Pro106Ala, and Pro106Thr) were found in some GR weeds [ 13 , 14 , 15 , 16 ]. Subsequently, the Thr102 mutation, the double mutation (Thr102 + Pro106) and even the triple mutation had been reported in GR weeds [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Multiple Resistance Mechanisms Involved in Glyphosate Resistance in Eleusine indica

Deng

Duan

et al. 2022

Plants

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Glyphosate is a non-selective herbicide and is widely used for weed control in non-cultivated land in China. One susceptible (S) and five putative glyphosate-resistant (R1, R2, R3, R4, and R5) Eleusine indica biotypes were selected to investigate their resistance levels and the potential resistance mechanisms. Based on the dose–response assays, the R3 and R5 biotypes showed a low-level (2.4 to 3.5-fold) glyphosate resistance, and the R1, R2, and R4 biotypes exhibited a moderate- to high-level (8.6 to 19.2-fold) resistance, compared with the S biotype. The analysis of the target-site resistance (TSR) mechanism revealed that the P106A mutation and the heterozygous double T102I + P106S mutation were found in the R3 and R4 biotypes, respectively. In addition, the similar EPSPS gene overexpression was observed in the R1, R2, and R5 biotypes, suggesting that additional non-target-site resistance (NTSR) mechanisms may contribute to glyphosate resistance in R1 and R2 biotypes. Subsequently, an RNA-Seq analysis was performed to identify candidate genes involved in NTSR. In total, ten differentially expressed contigs between untreated S and R1 or R2 plants, and between glyphosate-treated S and R1 or R2 plants, were identified and further verified with RT-qPCR. One ATP-binding cassette (ABC) transporter gene, one aldo-keto reductases (AKRs) gene and one cytochrome P450 monooxygenase (CytP450) gene were up-regulated in R1 or R2 plants. These results indicated that EPSPS overexpression, single or double mutation was a common TSR mechanisms in E. indica. Additional NTSR mechanisms could play an essential role in glyphosate resistance. Three genes, ABCC4, AKR4C10, and CYP88, could serve as important candidate genes and deserve further functional studies.

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First Report of Glyphosate-Resistant Biotype of Eleusine Indica (L.) Gaertn. in Europe

Cited by 6 publications

References 41 publications

Resistance of Eleusine indica to non-selective herbicides in Indonesian oil palm plantation

Resistance of Eleusine indica to non-selective herbicides in Indonesian oil palm plantation

Possibility of glyphosate-resistant goosegrass (Eleusine indica) in citrus fields in Rejang Lebong Districts, Bengkulu, Indonesia

Multiple Resistance Mechanisms Involved in Glyphosate Resistance in Eleusine indica

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