First report of grapevine virus L in grapevine in Turkey

İlbağı, Havva; Panailidou, Polina; Gagiano, Emcee; Pietersen, Gerhard; Maliogka, Varvara I.; Katis, N. I.; Çıtır, Ahmet

doi:10.1007/s42161-020-00668-2

Cited by 3 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Son yıllarda (2010'lu yıllardan sonra) uluslararası çalışmalara paralel olarak yeni belirlenen bağ virüslerinin (GVB; GVD; GVL; GPGV; GRLDaV; GRSPaV, GSyV-1 gibi) ve bağları enfekte eden diğer bir viroid olan AGVd'nin teşhisleri, hassas ve güvenilir teknikler kullanılarak bu zaman diliminde gerçekleştirilmiştir [47,18,37,17,24,74,16,72,75,44]. Moleküler çalışmaların yanında [30], serolojik çalışmalara da devam edilmiştir [45,61,73].…”

Section: Türkiye'de Asma Virüs çAlışmalarının Tarihsel Gelişimi Ve Karşılaşılan Sorunlarunclassified

Türkiye’de Belirlenen Bağ Virüs Hastalıklarının Tarihsel Gelişimi ve Önemi

Akbaş¹,

Morca²,

Coşkan³

2021

Bahçe

View full text Add to dashboard Cite

Türkiye bağcılığı sosyoekonomik anlamda en önemli ürün gruplarının arasında yer almaktadır. Bağlarda birçok zararlı organizma grubu özellikle de virüs enfeksiyonları bitkiyi zayıflatıp, verim ve kalitesinde azalmalara yol açtığından karantina ve sertifikasyonda başı çekmektedir. Son yarım asırda ülke bağ virüs araştırmalarında önemli çalışmalar yapılmış ve özellikle son 20 yılda virüslerin tanımı, karakterizasyonu ve mücadele yöntemleri alanında önemli ilerlemeler kat edilmiştir. Türkiye bağ yetiştiriciliği yapılan alanlarında şu ana kadar viral hastalıklara neden olan yaklaşık 23 virüs ve 5 viroidin varlığı belirlenmiştir. Arabis Mosaic Virüs (ArMV), Grapevine Fanleaf Virüs (GFLV), Grapevine Fleck Virüs (GFkV), Grapevine Leafroll-Associated Viruses (GLRaV-1, GLRaV-3) ve Grapevine Virüs A (GVA) en yaygın bağ virüsleri arasında yer almaktadır. Grapevine Virüs B (GVB), Grapevine Pinot Gris Virüs (GPGV), Grapevine Roditis Leaf Discoloration-Associated Virüs (GRLDaV), Grapevine Rupestris Stem Pitting-Associated Virüs (GRSPaV) ve Grapevine Syrah Virüs 1 (GSyV-1) gibi önemli viral etmenler de ülkede rapor edilmiştir. Türkiye’de bağ virüslerinin teşhisinde otsu-odunsu konukçulara indeksleme ve serolojik testler gibi geleneksel analiz yöntemlerinin yanında, moleküler analiz yöntemleri ve son dönemde yeni nesil dizileme teknolojisi (Next Generation Sequencing-NGS) gibi DNA dizileme tekniklerinin kullanımı ile teşhis ve karakterizasyon çalışmaları oldukça hızlanmıştır. Bu derlemede Türkiye’de bağ virüslerinin tarihsel gelişimi, oluşturduğu hastalıklar ve ekonomik önemi hakkında kısa bilgilere yer verilmiştir.

show abstract

Section: Türkiye'de Asma Virüs çAlışmalarının Tarihsel Gelişimi Ve Karşılaşılan Sorunlarunclassified

Türkiye’de Belirlenen Bağ Virüs Hastalıklarının Tarihsel Gelişimi ve Önemi

Akbaş¹,

Morca²,

Coşkan³

2021

Bahçe

View full text Add to dashboard Cite

show abstract

“…GVL is a newly identified member of the genus Vitivirus whose genome sequence was first identified in publicly available RNAseq libraries of grapevine samples from China, Croatia, USA (a Canadian grapevine sample), and New Zealand [3]. Since then, additional GVL isolates from grapevine samples from the USA (California and Texas) [13,14], Tunisia [15], Turkey [16], South Africa [17], Korea [18], and France [19] have been characterized. The GVL genome is 7,607 nt long and has a genome organization that is typical of members of the genus Vitivirus.…”

mentioning

confidence: 99%

“…For confirmation of the presence of GVL in single and composite samples, total RNA was extracted from leaf, petiole, or phloem tissue scrapings of each sample (depending on the sampling season), using the extraction method described above and the one-step reverse transcription polymerase chain reaction (RT-PCR) procedure described by Ilbagi et al [16], using a set of primers (GVL_F_6750/ GVL_R_6938, Table 1) that amplify a 189-bp-long fragment of the GVL CP gene (ORF4). One GVL isolate that was detected using the above reaction in all of the composite samples analyzed by HTS (GB15 from pool GeA, X10 from pool XA, and Ks14 from PKs1) and in the two isolates retrieved from the individual samples analyzed by HTS (AG-1 and AUTH69) were selected for confirmation of a larger part of the genome sequence by Sanger sequencing.…”

mentioning

confidence: 99%

“…Most of the collected samples (433) came from grafted Greek varieties, and 127 were collected from self-rooted Greek and foreign varieties (Table 2). All samples were tested for the presence of GVL using the one-step RT-PCR assay (Supplementary Text) described by Ilbağı et al [16] (Table 1).…”

mentioning

confidence: 99%

“…In this study, GVL was identified for the first time in Greek vineyards, thus further expanding our knowledge about the geographic distribution of this virus. Greece is the tenth country in which GVL has been detected, after Canada, Croatia, New Zealand, the USA [3,13,14], Tunisia [15], Turkey [16], South Africa [17], Korea [18], and France [19]. In addition, the identification of GVL has increased the number of vitiviruses that are known to be endemic in Greek vineyards to seven [20][21][22][23][24][25].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Identification and genetic diversity of grapevine virus L in Greece

Panailidou

Galeou

Pappi³

et al. 2023

Arch Virol

View full text Add to dashboard Cite

In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the tested samples, which originated from six viticultural areas of the country. Comparative sequence analysis based on the CP gene revealed a high degree of genetic variability among GVL isolates, while phylogenetic analysis grouped the Greek isolates in three of the five phylogroups formed, with most of them being classified in phylogroup I.

show abstract

Detection and diversity of grapevine virus L from a Vitis cultivar collection in Stellenbosch, South Africa

et al. 2021

Self Cite

View full text Add to dashboard Cite

A total of 229 Vitis cultivars were sampled from a collection in Stellenbosch, Western Cape, South Africa and subjected to an RNAtag-seq workflow and Illumina HiSeq 2500 sequencing.Following de novo assembly and initial BLASTn analysis of the resulting reads showed that 85 cultivars were infected with grapevine virus L (GVL), with a total of 96 complete/near complete genomes. This is the first time that GVL has been detected in South Africa.Phylogenetic analyses of the amino acid sequences of ORF1 showed that GVL from this study is diverse, grouping with references from Croatia, USA and China, as well as forming a unique South African phylogroup.

show abstract

First report of grapevine virus L in grapevine in Turkey

Cited by 3 publications

References 3 publications

Türkiye’de Belirlenen Bağ Virüs Hastalıklarının Tarihsel Gelişimi ve Önemi

Türkiye’de Belirlenen Bağ Virüs Hastalıklarının Tarihsel Gelişimi ve Önemi

Identification and genetic diversity of grapevine virus L in Greece

Detection and diversity of grapevine virus L from a Vitis cultivar collection in Stellenbosch, South Africa

Contact Info

Product

Resources

About