First report of ‘<i>Candidatus</i> Liberibacter solanacearum’ in the United Kingdom in the psyllid <i>Trioza anthrisci</i>

Sjölund, M. Jennifer; Clark, M. F.; Carnegie, Mairi; Greenslade, A. F. C.; Ouvrard, David; Highet, Fiona; Sigvald, Roland; Bell, James R.; Arnsdorf, Yvonne M.; Cairns, R.; Kenyon, D. M.

doi:10.5197/j.2044-0588.2017.036.004

Cited by 12 publications

(13 citation statements)

References 3 publications

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“…This psyllid was found on carrot and A. sylvestris from site 4. This supports the previous finding of Lso infected T. anthrisci in suction trap samples from Elgin 43 . Negative results for the presence this species across all other sites indicate that the T. anthrisci population in Scotland is localised, although the finding of Lso haplotype C in carrot plants at a site approx.…”

Section: Discussionsupporting

confidence: 93%

“…At site 4, T. anthrisci was the most abundant psyllid species; found on carrot plants and Anthriscus sylvestris. Trioza anthrisci were only collected from site 4 which is close to the suction trap location where Lso infected T. anthrisci were previously collected in Elgin 43 . Eight other species not known to feed on carrot were found on carrot crops but in low numbers (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Our understanding of the diversity, distribution and host range of Lso continues to expand rapidly; in most cases in response to symptomatic crops and associated losses. Although symptomatic crops have not been reported in the UK, the recent finding of Lso haplotype C in T. anthrisci from suction trap samples collected in Scotland 43 instigated this further study which aimed to better understand the risk posed to agriculture by examining the distribution of T. anthrisci, other psyllid vectors of Lso and potential natural plant reservoirs around agricultural sites. Our survey results give an indication of the natural distribution and diversity of Lso and associated plant/ insect hosts in the absence of a noticeable impact on agriculture.…”

Section: Discussionmentioning

confidence: 99%

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‘Candidatus Liberibacter solanacearum’ distribution and diversity in Scotland and the characterisation of novel haplotypes from Craspedolepta spp. (Psylloidea: Aphalaridae)

Sumner-Kalkun¹,

Highet²,

Arnsdorf³

et al. 2020

Sci Rep

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The phloem limited bacterium ‘Candidatus Liberibacter solanacearum’ (Lso) is associated with disease in Solanaceous and Apiaceous crops. This bacterium has previously been found in the UK in Trioza anthrisci, but its impact on UK crops is unknown. Psyllid and Lso diversity and distribution among fields across the major carrot growing areas of Scotland were assessed using real-time PCR and DNA barcoding techniques. Four Lso haplotypes were found: C, U, and two novel haplotypes. Lso haplotype C was also found in a small percentage of asymptomatic carrot plants (9.34%, n = 139) from a field in Milnathort where known vectors of this haplotype were not found. This is the first report of Lso in cultivated carrot growing in the UK and raises concern for the carrot and potato growing industry regarding the potential spread of new and existing Lso haplotypes into crops. Trioza anthrisci was found present only in sites in Elgin, Moray with 100% of individuals harbouring Lso haplotype C. Lso haplotype U was found at all sites infecting Trioza urticae and at some sites infecting Urtica dioica with 77.55% and 24.37% average infection, respectively. The two novel haplotypes were found in Craspedolepta nebulosa and Craspedolepta subpunctata and named Cras1 and Cras2. This is the first report of Lso in psyllids from the Aphalaridae. These new haplotypes were most closely related to Lso haplotype H recently found in carrot and parsnip. Lso was also detected in several weed plants surrounding carrot and parsnip fields. These included two Apiaceous species Aegropodium podagraria (hap undetermined) and Anthriscus sylvestris (hap C); one Gallium sp. (Rubiaceae) (hap undetermined); and Chenopodium album (Amaranthaceae) (hap undetermined).

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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‘Candidatus Liberibacter solanacearum’ distribution and diversity in Scotland and the characterisation of novel haplotypes from Craspedolepta spp. (Psylloidea: Aphalaridae)

Sumner-Kalkun¹,

Highet²,

Arnsdorf³

et al. 2020

Sci Rep

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“…We explored incorporating four taxa into the phylogeny for which we had short bait sequences (cox1 and cytB). The taxa selected were two Trioza species ( T. apicalis Foerster and T. flixiana Burckhardt & Lauterer); T. apicalis is considered a serious pest of carrot crops in northern and central Europe and is also suspected of vectoring pathogens (Nissinen et al ., ); more recently the sister taxon T. anthrisci Burckhardt was a confirmed carrier and potential vector of the proteobacterium ‘ Candidatus Liberibacter solanacearum’ (Sjölund et al ., ). Secondly, a taxon in the genus Diclidophlebia Crawford [ D. fremontiae (Klyver)] is of particular interest as the single North American species in an otherwise mostly tropical and subtropical group (distributed worldwide) with a complex taxonomic history (Ouvrard, ).…”

Section: Methodsmentioning

confidence: 97%

Resolving the psyllid tree of life: phylogenomic analyses of the superfamily Psylloidea (Hemiptera)

Percy

Crampton‐Platt

Sveinsson

et al. 2018

Systematic Entomology

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Understanding evolutionary relationships in the superfamily Psylloidea is challenging due to the lack of clear morphological synapomorphies for many groups. Some families and many of the genera, including the two largest, Cacopsylla Ossiannilsson and Trioza Foerster, have long been acknowledged as nonmonophyletic and the circumscription of natural groups has remained fluid. We present the best phylogenetic hypothesis to date for Psylloidea and provide a working systematic framework to better reflect evolutionary relationships. A shotgun sequencing approach using mixed pool DNAs for more than 400 species resulted in recovery from de novo assemblies of near-complete mitogenomes (≥10 kb) for 359 species, and partial genomes (5-10 kb) for an additional 40 species. The resulting phylogeny improves and clarifies the family classification and resolves some of the longstanding uncertainties in relationships within and between genera. A whole-nuclear-genome scan approach (yielding data from an estimated 373 nuclear genes) using the anchored hybrid enrichment method for a representative subset of taxa confirms the placement of major groupings and overall tree topology recovered with the mitochondrial data. The data generated represent a major increase in molecular resources for this superfamily. In addition, we highlight areas of remaining uncertainty that require further sampling and/or additional sources of data. The phylogeny provides new insights for both evolutionary and applied research, and a backbone constraint tree allows the placement of taxa of particular interest or concern (e.g. pest taxa) with only small fragments of sequence available (e.g. DNA barcodes).

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“…B. trigonica does not occur in northern Europe, and B. nigricornis has only been observed twice in Finland and both these records are old (Aulmann 1913;Lindberg & Ossiannilsson 1960). Recently, Sjölund et al (2017) found that Trioza anthrisci carries CLso haplotype C in Scotland and Sweden. In Finland, T. anthrisci is a very common species feeding on cow parsley, Anthriscus sylvestris.…”

Section: Discussionmentioning

confidence: 99%

Carrot Pathogen ‘Candidatus Liberibacter solanacearum’ Haplotype C Detected in Symptomless Potato Plants in Finland

et al. 2018

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Candidatus Liberibacter solanacearum' (CLso) haplotype C, a bacterial pathogen transmitted by the carrot psyllid Trioza apicalis, causes yield losses in carrot production. Due to concerns that this pathogen might also threaten potato (Solanum tuberosum) production, the occurrence of CLso in cultivated and volunteer potatoes in Tavastia Proper and Satakunta regions of Finland was studied. Volunteer potato plants were found in 13 of the 27 inspected carrot fields. Of the 148 potato samples tested by PCR, eight volunteer potato plants and one cultivated potato grown at the edge of a carrot field were found CLso positive. The PCR products obtained from these potatoes with primers OA2/OI2c, LpFrag4-1611F/LpFrag4-480R and CL514F/CL514R all showed 100% sequence identity to CLso haplotype C. This is the first observation of CLso haplotype C in field-grown potatoes. In addition, transmission experiments were performed. Attempts to transmit CLso into potato with carrot psyllids were not successful; however, CLso haplotype C was transmitted from infected carrots to potato plants by leaf grafting and by phloem connection formed by dodder, a parasitic plant, and found to survive in the potato plants for several weeks after transmission. However, the bacterial colonization progressed slowly in the potato phloem, and the amount of bacteria detected was low. The plants produced from the daughter tubers of the CLso positive potato plants were all CLso negative, suggesting that CLso haplotype C was not able to pass to the daughter plants. None of the CLso positive potatoes inoculated in greenhouse or collected from fields showed symptoms characteristic of zebra chip disease, associated with CLso haplotypes A and B.

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First report of ‘Candidatus Liberibacter solanacearum’ in the United Kingdom in the psyllid Trioza anthrisci

Cited by 12 publications

References 3 publications

‘Candidatus Liberibacter solanacearum’ distribution and diversity in Scotland and the characterisation of novel haplotypes from Craspedolepta spp. (Psylloidea: Aphalaridae)

‘Candidatus Liberibacter solanacearum’ distribution and diversity in Scotland and the characterisation of novel haplotypes from Craspedolepta spp. (Psylloidea: Aphalaridae)

Resolving the psyllid tree of life: phylogenomic analyses of the superfamily Psylloidea (Hemiptera)

Carrot Pathogen ‘Candidatus Liberibacter solanacearum’ Haplotype C Detected in Symptomless Potato Plants in Finland

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