First report of <i>Chili pepper mild mottle virus</i> in calibrachoa in the United States

Groth‐Helms, Deborah; Juszczak, Samantha; Adkins, Scott

doi:10.1002/ndr2.12120

Cited by 6 publications

(1 citation statement)

References 9 publications

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“…Since major outbreaks in the 1990s, tobamoviruses still frequently occur in petunia and calibrachoa; sometimes detection methods fail to detect a new isolate or new tobamovirus-host plant combinations occur. In 2003, a strain of TMV in Petunia was not detected by enzyme-linked immunosorbent assay (ELISA) but was picked up by indicator plant research (93); turnip vein clearing virus (108) and another TMV variant was detected in Petunia (63), and bell pepper mottle virus (85) and chili pepper mild mottle virus were detected in Calibrachoa (47).…”

Section: Tobamoviruses In Petunia and Calibrachoamentioning

confidence: 99%

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

Huang

Jordan

Meekes³

et al. 2023

Annu. Rev. Phytopathol.

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Since the 1950s, there have been major changes in the scope, value, and organization of the ornamental plant industry. With fewer individual producers and a strong trend toward consolidation and globalization, increasing quantities of diverse plant genera and species are being shipped internationally. Many more ornamentals are propagated vegetatively instead of by seed, further contributing to disease spread. These factors have led to global movement of pathogens to countries where they were not formerly known. The emergence of some previously undescribed pathogens has been facilitated by high-throughput sequencing, but biological studies are often lacking, so their roles in economic diseases are not yet known. Case studies of diseases in selected ornamentals discuss the factors involved in their spread, control measures to reduce their economic impact, and show some potential effects on agronomic crops. Advances in diagnostic techniques are discussed, and parallels are drawn to the international movement of human diseases. Expected final online publication date for the Annual Review of Phytopathology, Volume 61 is September 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Tobamoviruses In Petunia and Calibrachoamentioning

confidence: 99%

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

Huang

Jordan

Meekes³

et al. 2023

Annu. Rev. Phytopathol.

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Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes

Ontañón,

Ojinaga,

Larregla

et al. 2023

Eur J Plant Pathol

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An isolate of chili pepper mild mottle virus (CPMMV-Sp; GenBank OQ920979) with a 99% identity to CPMMV (GenBank MN164455.1) was found in symptomatic pepper plants in Spain. RACE analysis, performed using a stem-loop primer developed in this study to prime at the end of the introduced poly(A)/(U) tail, revealed the presence of an extra 22 nt at the 5' end, starting with a cytosine, which were essential to generate infectious clones. However, the 5' terminal cytosine was dispensable for initiating the infection. The design of two specific digoxigenin riboprobes targeting the more divergent area of CPMMV-Sp, compared to the closely related bell pepper mottle virus (BPeMV) (identity percentage of 80.6% and 75.8%, respectively), showed that both probes specifically detected CPMMV-Sp when the hybridization was performed at 68ºC and 60ºC, respectively. However, the BPeMV probe, targeting a region with an 89.4% identity percentage to CPMMV-Sp, showed cross-hybridization at 60ºC but not at 68ºC. The comparison of the detection limits between molecular hybridization and RT-PCR techniques revealed that the former was 125 times less sensitive than RT-PCR. The analysis of the vertical transmission of CPMMV-Sp using seeds from naturally or mechanically infected pepper plants revealed a transmission percentage ranging from 0.9% to 8.5%. Finally, the analysis of the resistance of capsicum species carrying different alleles of the L gene (L1, L2, L3, and L4) revealed that varieties with the L1 gene were infected by CPMMV-Sp (20–40% of inoculated plants), while varieties with the L2, L3, and L4 genes were resistant.

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Meristem culture is a quite efficient method for the eradication of Potato Virus Y (PVY) from calibrachoa ‘Pampa Salmon INTA’

Leticia,

Silvina,

Ángel

et al. 2024

Plant Cell Tiss Organ Cult

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First report of Chili pepper mild mottle virus in calibrachoa in the United States

Cited by 6 publications

References 9 publications

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes

Meristem culture is a quite efficient method for the eradication of Potato Virus Y (PVY) from calibrachoa ‘Pampa Salmon INTA’

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