First Report of <i>Gnomoniopsis smithogilvyi</i> Causing Chestnut Brown Rot on Chestnut Fruit in Michigan

Sakalidis, Monique L.; Medina-Mora, C.; Kolp, Matthew; Fulbright, D. W.

doi:10.1094/pdis-03-19-0562-pdn

Cited by 14 publications

(20 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So far, eleven Gnomoniopsis species were reported from fagaceous hosts, of which G. clavulata and G. paraclavulata were described from Quercus in the USA [20]. Gnomoniopsis smithogilvyi was reported as causal agent of sweet chestnut fruit rot in Australia, Europe and North America [25,26,[41][42][43][44][45]. The remaining eight species are only known from So far, eleven Gnomoniopsis species were reported from fagaceous hosts, of which G. clavulata and G. paraclavulata were described from Quercus in the USA [20].…”

Section: Discussionmentioning

confidence: 99%

“…The remaining eight species are only known from So far, eleven Gnomoniopsis species were reported from fagaceous hosts, of which G. clavulata and G. paraclavulata were described from Quercus in the USA [20]. Gnomoniopsis smithogilvyi was reported as causal agent of sweet chestnut fruit rot in Australia, Europe and North America [25,26,[41][42][43][44][45]. The remaining eight species are only known from China.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

Jiang

Voglmayr

Bian

et al. 2021

JoF

View full text Add to dashboard Cite

Gnomoniopsis (Gnomoniaceae, Diaporthales) is a well-classified genus inhabiting leaves, branches and fruits of the hosts in three plant families, namely Fagaceae, Onagraceae and Rosaceae. In the present study, eighteen Gnomoniopsis isolates were obtained from diseased leaves of Fagaceae hosts collected from Fujian, Guangdong, Hainan, Henan, Jiangxi and Shaanxi provinces in China. Morphology from the cultures and phylogeny based on the 5.8S nuclear ribosomal DNA gene with the two flanking internally transcribed spacer (ITS) regions, the translation elongation factor 1-alpha (tef1) and the beta-tubulin (tub2) genes were employed to identify these isolates. As a result, seven species were revealed, viz. Gnomoniopsis castanopsidis, G. fagacearum, G. guangdongensis, G. hainanensis, G. rossmaniae and G. silvicola spp. nov, as well as a known species G. daii. In addition, G. daii was firstly reported on the host Quercus aliena.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

Jiang

Voglmayr

Bian

et al. 2021

JoF

View full text Add to dashboard Cite

show abstract

“…The disease severely impacts fruit production and marketing in Europe with very high incidence, as for Switzerland with 91% of infected fruits (Dennert et al, 2015) and Italy, up to 93.5% (Lione et al, 2015). Brown rot of kernels is also reported in Australia (Shuttleworth et al, 2013), North America (Sakalidis et al, 2019) and Chile (Vannini, unpublished).Gnomoniopsis castaneae can also incite shoot blight and leaf necroses on chestnut as well as bark cankers on both chestnut (Dar & Rai, 2015) and hazelnut (Corylus avellana), as exhaustively reviewed by Lione et al, (2019). The ecology, biology and epidemiology of this fungus are particularly complex.…”

mentioning

confidence: 99%

“…

is an emerging fungal pathogen causing brown rot of Castanea sativa, C. mollissima and the hybrids C. sativa x C. crenata (Sakalidis et al, 2019). The disease severely impacts fruit production and marketing in Europe with very high incidence, as for Switzerland with 91% of infected fruits (Dennert et al, 2015) and Italy, up to 93.5% (Lione et al, 2015).

…”

mentioning

confidence: 99%

See 1 more Smart Citation

Development of a TaqMan qPCR assay for the detection and quantification of Gnomoniopsis castaneae in chestnut tissues

et al. 2021

View full text Add to dashboard Cite

A novel real‐time PCR assay based on the TaqMan probe was developed for the detection of Gnomoniopsis castaneae, causal agent of brown rot of chestnut kernels, and responsible for leaf necrosis, shoot blight and bark canker. A part of the pathogen life cycle is endophytic, colonizing all tissues of chestnut and additional hosts, which is suspected to play a key role in its epidemiology. Thus, a molecular tool for sensitive detection and quantification of G. castaneae in symptomatic and asymptomatic host tissues is urgently required to better understand G. castaneae ecology, biology and epidemiology. Primers and a species‐specific probe for G. castaneae were designed based on the sequence of the single‐copy elongation factor 1 alpha (EF1α) gene. The amplification efficiency of target DNA was 105.3% and the limit of detection of the assay was calculated at approximately 40 fg of pure fungal DNA. The pathogen was consistently detected in artificial mixtures of plant and pathogen DNAs with the same Limit of Detection (LOD) as pure fungal DNA. In naturally infected samples, the assay rapidly revealed the presence of the pathogen in all symptomatic specimens, as well as in asymptomatic tissues. Notably, a significant relationship between the results of a metagenomic HTS analysis and the qPCR assay on DNAs extracted from bulk fruit was found. This molecular tool will be of substantial aid in detecting and quantifying G. castaneae, even in the endophytic state, and in different host tissues.

show abstract

Impact of ‘brown rot’ caused by Gnomoniopsis castanea on chestnut fruits during the post‐harvest process: critical phases and proposed solutions

et al. 2021

View full text Add to dashboard Cite

BACKGROUND The brown rot fungus, Gnomoniopsis castanea, is the main organism responsible for the outbreak of chestnut postharvest decay that is threatening the sustainability of the chestnut market in Europe. Currently, no specific strategy is available to mitigate the impact and remediate the high losses of fruits in postharvest storage. In the present study, the different phases of chestnut handling in a standard facility plant were analyzed by evaluating the amount of fruit rot and infection by G. castanea at each phase. RESULTS The warm bath (48 °C) was identified as the critical phase, requiring strict parametrization to effectively inactivate G. castanea in fruits. Laboratory tests indicated that maintaining fruits at 50 °C for a maximum of 45 min provided optimal conditions to completely inactivate G. castanea inoculum during postharvest handling. However, the warm bath at 50 °C and over was not effective in inactivating the complex of fungal taxa responsible for contamination and development of molds. Higher temperatures and extended treatment times caused significant losses in fruit quality, as indicated by taste panel evaluation. Upscaling of postharvest facilities is discussed and critically evaluated. CONCLUSION The warm bath (50 °C for 45 min) is effective in completely inactivating G. castanea in fruits but did not reduce the impacts of the complex of molds responsible for external contamination and mycotoxin production. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

show abstract

First Report of Gnomoniopsis smithogilvyi Causing Chestnut Brown Rot on Chestnut Fruit in Michigan

Cited by 14 publications

References 5 publications

Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

Morphology and Phylogeny of Gnomoniopsis (Gnomoniaceae, Diaporthales) from Fagaceae Leaves in China

Development of a TaqMan qPCR assay for the detection and quantification of Gnomoniopsis castaneae in chestnut tissues

Impact of ‘brown rot’ caused by Gnomoniopsis castanea on chestnut fruits during the post‐harvest process: critical phases and proposed solutions

Contact Info

Product

Resources

About