First report of <i>Pantoea ananatis</i> causing grain discolouration and leaf blight of rice in Russia

Егорова, М.А.; Mazurin, E. S.; Игнатов, А. Н.

doi:10.5197/j.2044-0588.2015.032.021

Cited by 23 publications

(18 citation statements)

References 2 publications

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“…None of existing multiplex PCR assay have focused on the simultaneous detection of these pathogens in Africa. Also none of them included the two bacterial genera Pantoea and Sphingomonas that were recently shown to cause severe symptoms on rice, in West Africa [14,17,18], but also in other countries [36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Bangratz

Wonni

Kini³

et al. 2020

PLoS ONE

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Crop diseases are responsible for considerable yield losses worldwide and particularly in sub-Saharan Africa. To implement efficient disease control measures, detection of the pathogens and understanding pathogen spatio-temporal dynamics is crucial and requires the use of molecular detection tools, especially to distinguish different pathogens causing more or less similar symptoms. We report here the design a new molecular diagnostic tool able to simultaneously detect five bacterial taxa causing important diseases on rice in Africa: (1) Pseudomonas fuscovaginae, (2) Xanthomonas oryzae, (3) Burkholderia glumae and Burkholderia gladioli, (4) Sphingomonas and (5) Pantoea species. This new detection tool consists of a multiplex PCR, which is cost effective and easily applicable. Validation of the method is presented through its application on a global collection of bacterial strains. Moreover, sensitivity assessment for the detection of all five bacteria is reported to be at 0.5 ng DNA by μl. As a proof of concept, we applied the new molecular detection method to a set of 256 rice leaves collected from 16 fields in two irrigated areas in western Burkina Faso. Our results show high levels of Sphingomonas spp. (up to 100% of tested samples in one field), with significant variation in the incidence between the two sampled sites. Xanthomonas oryzae incidence levels were mostly congruent with bacterial leaf streak (BLS) and bacterial leaf blight (BLB) symptom observations in the field. Low levels of Pantoea spp. were found while none of the 256 analysed samples was positive for Burkholderia or Pseudomonas fuscovaginae. Finally, many samples (up to 37.5% in one studied field) were positive for more than one bacterium (co-infection). Documenting co-infection levels are important because of their drastic consequences on epidemiology, evolution of pathogen populations and yield losses. The newly designed multiplex PCR for multiple bacterial pathogens of rice is a significant improvement for disease monitoring in the field, thus contributing to efficient disease control and food safety.

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Section: Introductionmentioning

confidence: 99%

Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Bangratz

Wonni

Kini³

et al. 2020

PLoS ONE

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“…Pantoea ananatis has a cosmopolitan distribution and has been found right across the globe, from South Africa in the south (Coutinho et al ., ; Goszczynska et al ., ; Weller‐Stuart et al ., ) to Russia in the north (Egorova et al ., ), Mexico in the west (Perez‐y‐Terron et al ., ) and Australia in the east (Murrell et al ., ). In these areas, this bacterium has an exceptional lifestyle, as it is not only associated with plants, but is also frequently isolated from a wide range of environmental sources.…”

Section: Introductionmentioning

confidence: 99%

Pantoea ananatis: genomic insights into a versatile pathogen

Weller-Stuart

Maayer

Coutinho

2017

Molecular Plant Pathology

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“…are recognized as being responsible for several diseases of plants, including important crop plants such as rice, maize, sorghum, onion and melon [34–43]. BB of rice caused by species of Pantoea were reported in several countries and include Benin, Togo, Korea, India, Australia, China, Italy, Venezuela, and Russia [10,11,40,44–49].…”

Section: Discussionmentioning

confidence: 99%

A diagnostic multiplex PCR scheme for identification of plant-associated bacteria of the genusPantoea

Kini

Agnimonhan

Dossa

et al. 2018

Preprint

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12 13 Background: The genus Pantoea forms a complex of more than 25 species, among which 14 several cause diseases of several crop plants, including rice. Notably, strains of Pantoea 15 ananatis and Pantoea stewartii have been found to cause bacterial leaf blight of rice in Togo 16 and Benin, while other authors have observed that Pantoea agglomerans can also cause 17 bacterial leaf blight of rice. The contribution of these and perhaps other species of Pantoea to 18 plant diseases and yield losses of crop plants is currently not well documented, partly due to 19 the lack of efficient diagnostic tools. 20 21 Result: Using 34 whole genome sequences of the three-major plant-pathogenic Pantoea 22species, a set of PCR primers that specifically detect each of the three species, 23 P. agglomerans, P. ananatis, and P. stewartii, was designed. A multiplex PCR protocol which 24 can distinguish these three species and also detects members of other Pantoea species was 25 2 further developed. Upon validation on a set of reference strains, 609 suspected Pantoea 26 strains that were isolated from rice leaves or seeds originating from 11 African countries were 27 screened. In total, 41 P. agglomerans strains from eight countries, 79 P. ananatis strains from 28 nine countries, 269 P. stewartii strains from nine countries and 220 unsolved Pantoea strains 29 from ten countries were identified. The PCR protocol allowed detecting Pantoea bacteria 30 grown in vitro, in planta and in rice seeds. The detection threshold was estimated at 5 ng/mL 31 of total genomic DNA and 1×10 5 CFU/mL of heated cells. 32 33 Conclusion: This new molecular diagnostic tool will help accurately diagnose major plant-34 pathogenic species of Pantoea. Due to its robustness, specificity, sensitivity, and cost 35 efficiency it will be very useful for plant protection services and for the epidemiological 36 surveillance of these important crop-threatening bacteria. 37 38 39 41 3 Background 42 43 The genus Pantoea was first described in 1989 and was recently taxonomically classified as a 44 member of the Erwiniaceae family [1]. More than 25 species of this genus have been 45 described and reported worldwide [2,3]. Etymologically, the genus name Pantoea is derived 46 from the Greek word 'Pantoios', which means "of all sorts or sources" and reflects the diverse 47 geographical and ecological sources from which the bacteria have been isolated. Several 48 species of the genus are qualified as versatile and ubiquitous bacteria because they have been 49 isolated from many different ecological niches and hosts [2,4]. Remarkably, some species 50 have the ability to colonize and interact with members of both the plant and the animal 51 Kingdom [5]. Among the plant-interacting species, Pantoea ananatis, Pantoea agglomerans 52 and Pantoea stewartii are well known for their phytopathogenic characteristics. They are 53 recognized as the causal agent of several diseases, such as leaf blight, spot disease, dieback, 54 grain discoloration, seed stalk rot, center rot, stem necros...

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First report of Pantoea ananatis causing grain discolouration and leaf blight of rice in Russia

Cited by 23 publications

References 2 publications

Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

Pantoea ananatis: genomic insights into a versatile pathogen

A diagnostic multiplex PCR scheme for identification of plant-associated bacteria of the genusPantoea

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