First report of Pepino mosaic virus in tomato in Italy

Differential Tomato Transcriptomic Responses Induced by Pepino Mosaic Virus Isolates with Differential Aggressiveness

“…The virus causes a wide range of symptoms, of which the typical fruit marbling is particularly devastating, as it reduces the economic value of the fruit (Soler et al, 2000;Roggero et al, 2001;Spence et al, 2006;Hanssen et al, 2009Hanssen et al, , 2010a. Damage and economic losses caused by PepMV vary greatly and can at least partially be attributed to the differential symptomatology caused by different PepMV isolates (Hanssen et al, 2009(Hanssen et al, , 2010a.…”

Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination

“…PepMV was first identified in Peru in 1974 from young leaves of pepino (Solanum muricatum) that displayed yellow mosaic symptoms (Jones et al 1980). Presence of the virus in tomato crops was not reported until 1999 when it was found in Dutch tomatoes (Van der Vlugt et al 2000), after which the virus quickly spread in tomato crops throughout Europe and North America (Soler et al 2000;French et al 2001;Jorda et al 2001;Mumford and Metcalfe 2001;Roggero et al 2001). A wide range of symptoms has been associated with PepMV infection in tomatoes including leaf mosaic, leaf distortions, nettle heads and stunting.…”

“…A wide range of symptoms has been associated with PepMV infection in tomatoes including leaf mosaic, leaf distortions, nettle heads and stunting. Apart from these, fruit discoloration, mostly expressed as marbling or flaming, caused by irregular lycopene distribution, is considered as the most devastating result of PepMV infection because it reduces the commercial value of the fruit (Soler et al 2000;Mumford and Metcalfe 2001;Roggero et al 2001;Spence et al 2006). In some cases, even complete plant collapse has been associated with PepMV infection (Soler-Aleixandre et al 2005).…”

Epidemiología y variabilidad patogénica del virus del mosaico del pepino dulce (Pepino mosaic virus). Nuevas enfermedades asociadas a su presencia (torrao o cribado)

“…Posteriormente, se ha determinado su presencia en cultivos de tomate de diferentes países como Italia, concretamente en las islas de Cerdeña (Roggero et al, 2001), y Sicilia (Davino et al, 2006), Francia (Cotillon et al, 2002), Austria, Bélgica, Noruega, Finlandia, Ucrania (Verhoeven et al, 2003), Chile (Ramírez y Bustamante, 2001;Muñoz et al, 2002), Hungría (Forray et al, 2004), China (Zhang et al, 2003), Perú (Soler et al, 2002), Suecia, Dinamarca, Polonia, Bulgaria, Eslovaquia, Marruecos, Guatemala, Ecuador e Irlanda (EPPO, 2009).…”

“…Esta técnica fue empleada en los primeros diagnósticos del virus, determinándose la presencia de aislados de la cepa EU de PepMV, aunque la concentración de virus detectado fue menor que la obtenida con el aislado original del virus (French et al, 2001;Roggero et al, 2001 complementarios a un fragmento del DNA molde y del que se amplificarán múltiples copias en la reacción empleando una DNA polimerasa termo estable (Hull, 2002). A lo largo de los años, se han publicado diversas secuencias de cebadores degenerados diseñados en zonas conservadas comunes a diferentes virus dentro del género Potexvirus (Gibbs et al, 1998; van der Vlugt y Berendsen, 2002), así como diversas secuencias de cebadores específicos de PepMV, que hibridan con fragmentos del genoma de algún aislado o cepa concreta de PepMV, o son generales de la especie (Mumford y Metcalfe, 2001;French et al, 2001;Martínez-Culebras et al, 2002;Aguilar et al, 2002;Cotillon et al, 2002;López et al, 2005;Pagán et al, 2006;Ling, 2007;AlfaroFernández et al, 2008a;Ling et al, 2008;Hanssen et al, 2009b).…”