First Report of Tomato yellow leaf curl virus Associated with Beans, Phaseolus vulgaris, in Cuba

Abstract

Beans with yellow mosaic and/or leaf crumple symptoms were collected in three fields in the southern area of the province of Havana, Cuba in December 2001 and February 2002. DNA was extracted from the fresh bean leaves of 25 samples (1). Dot blot hybridization was performed at high stringency with a specific probe for Tomato yellow leaf curl virus (TYLCV). The specific probe was prepared by alkaline phosphatase labeling of the polymerase chain reaction (PCR) fragment amplified with primer pair, PTYIRv21/PTYIRc287, containing the intergenic region (IR) of TYLCV, and chemiluminescent hybridization was completed as described by the manufacturer (AlkPhos Direct Labeling and Detection Systems, Amersham Pharmacia Biotech Inc., Piscataway, NJ). Four of the samples had positive hybridization signals. PCR was performed with overlapping primers for TYLCV (2) with the DNA extract from sample 01–44, which gave a positive hybridization signal with the TYLCV probe, and a 2.8-kb fragment was obtained. This fragment was cloned in pGem T-Easy (pBeTY44) and partially sequenced. Greater than 96% nt identity was obtained for the 591 nt of the IR and 504 nt of the N-terminus of the Rep gene with TYLCV (GenBank Accession No. AF260331). Also, PCR was completed on 11 of the 25 samples with the degenerate primer pair PAL1v1978/PAR1c715 for DNA-A (3). Eight samples gave fragment sizes of 1.4 kb and one sample gave a fragment of 1.3 kb. The 1.3-kb fragment from sample number 01–50 was cloned in pGem T-Easy (pBeBG50) and partially sequenced. Pairwise nucleotide comparisons with Bean golden yellow mosaic virus (BGYMV, GenBank Accession No. M91604) were 95% for 719 nt of the N-terminus of the Rep gene. These results are consistent with the association of both TYLCV and BGYMV in beans and have important implications for future disease management strategies. References: (1) G. P. Accotto et al. Eur. J. Plant. Pathol. 106:179, 2000. (2) M. K. Nakhla et al. Plant Dis. 78:926, 1994. (3) M. Rojas et al. Plant Dis. 77:340, 1993.

Bemisia: Bionomics and Management of a Global Pest

“…Although the viruses associated with TYLCD affect primarily tomato, natural infections have been reported in other cultivated hosts such as common bean (Gorsane et al 2004;Martínez-Zubiaur et al 2002;Navas-Castillo et al 1999), pepper (Gorsane et al 2004;Quiñones et al 2002;, Reina et al 1999, tobacco (Font et al 2005 Davino et al 1994;Bedford et al 1998;Sánchez-Campos et al 1999Jordá et al 2001;Salati et al 2002).…”

“…This virus rapidly spread on Hispaniola, paralyzing the tomato paste industry in the Dominican Republic and Haiti (Morales 2006a;. This virus is not only a major pathogen of tomatoes, but can also infect other cultivated species such as peppers, tobacco, common bean, and cucurbits (Dalmon and Marchoux 2000;Martinez-Zubiaur et al 2002. In the late 1990s, TYLCV was detected in Yucatan, Mexico ).…”

Primer reporte de begomovirus infectando cultivos de ají (<i>Capsicum</i> spp.) en Colombia

“…Este resultado indica que el virus detectado en ají es diferente a PYMV, un begomovirus que afecta tomate en Colombia. Esto es interesante, ya que muchos de los begomovirus que infectan tomate se han reportado que afectan ají; por ejemplo, en Trinidad y Tobago, y en la Isla Guadalupe se ha reportado que PYMV afecta Capsicum (Umaharan et al, 1998;Urbino et al, 2004); en Cuba, TYLCV se ha detectado en cultivos de ají y frijol (Quiñones et al, 2001;Martínez et al, 2002); en Perú se reportó que PepLRV infecta cultivos de ají, frijol y tomate (Martínez et al, 2013); y en México, se ha reportado que PHYVV y PepGMV afectan tomate y ají (Torres et al, 1993;Lotrakul et al, 2000). En la Tabla 1, se observa que la mayor cantidad de muestras positivas para begomovirus se presentó en la zona Centro-Sur del Valle del Cauca, representada por los municipios de Tuluá, Guacarí, Vijes, Palmira y Candelaria; en donde se colectaron 84 muestras de ají (habanero, 48; tabasco, 25; cayena, diez; jalapeño, uno), y de las cuales 78 fueron positivas con un porcentaje de incidencia de 92,8 %.…”