First report of Xiphinema hunaniense Wang &amp; Wu, 1992 (Nematoda: Longidoridae) in Vietnam

Nguyen, Huu Tien; Nguyen, Thi Duyen; Le, Thi Mai Linh; Trinh, Quang Phap

doi:10.21307/jofnem-2020-078

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…Especially, 18S, 28S, and ITS rDNA regions were the most frequently used molecular barcodes in identifying Xiphinema spp. (Nguyen et al, 2020;Orlando et al, 2016;Wang et al, 2003;Wu et al, 2007). This study was able to amplify 18S and 28S rDNA sequences of X. hunaniense on tea in Vietnam and deposited to GenBank.…”

Section: Xiphinema Hunaniensementioning

confidence: 99%

First record of Xiphinema hunaniense Wang & Wu, 1992 (Dorylaimida: Longidoridae) associated with tea (Camellia sinensis (L.) Kuntze) in Thanh Hoa, Vietnam

Linh¹,

Nguyen²,

Duyen³

et al. 2021

AJB

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The dagger nematodes, Xiphinema spp., are migratory root-ectoparasitic nematodes that cause damage to a wide range of wild and cultivated plants over the world. In Vietnam, this nematode group has been studied mainly based on morphological characterizations. During a survey of pathogens associated with tea, a plant with many medicinal and therapeutic potentials, a population of Xiphinema hunaniense was recorded. This study provides the first morphological and molecular characterizations of Xiphinema hunaniense found on Tea in Vietnam. The 28S rDNA, and 18S rDNA phylogenetic trees of the genus Xiphinema are also provided. 18S rDNA sequence of X. hunaniense is also submitted to GenBank for the first time.

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Section: Xiphinema Hunaniensementioning

confidence: 99%

First record of Xiphinema hunaniense Wang & Wu, 1992 (Dorylaimida: Longidoridae) associated with tea (Camellia sinensis (L.) Kuntze) in Thanh Hoa, Vietnam

Linh¹,

Nguyen²,

Duyen³

et al. 2021

AJB

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“…The D2-D3 region of 28S rRNA was amplified using D2A (5′-ACA AGT ACC GTG AGG GAA AGTTG-3′) and D3B (3′-TCC TCG GAA GGA ACC AGC TACTA-5′) primers (De Ley et al 1999). The obtained sequence was analyzed following Nguyen et al (2020). Blast search was used to search for closely related species on GenBank (Altschul et al 1997).…”

Section: Molecular Characterizationmentioning

confidence: 99%

“…Forward and reverse sequences were assembled and analysed using Geneious R11. The best fit model was chosen using Mega 7, following Nguyen et al (2020). Phylogenetic trees were created using MrBayes 3.2.6 Add-in in Geneious R11 under GTR + G + I model (Markov chains were set with 1 × 106 generations, four runs, 20% burn-in, and subsampling frequency of 500 generations) following Nguyen et al (2020).…”

Section: Molecular Characterizationmentioning

confidence: 99%

First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam

Trinh

Nguyen

et al. 2021

Egypt J Biol Pest Control

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Background Entomopathogenic nematodes (EPNs) are evidently a useful nematode group for the biocontrol of insect pests. It is well known that efficacy of different EPN strains, even belonging to the same species, can be significantly varied in different localities. Therefore, exploring EPNs and testing their efficacy in various ecological regions is of crucial importance to find out more efficient EPN strains. On the other hand, this practice is also needed to enhance the knowledge on diversity and distribution model of EPNs over the world. Results In this study, a species belonging to the genus Steinernema, S. surkhetense, has been characterized for the first time in Vietnam based on morphological and molecular characterizations. Morphological characterizations of infective juveniles, the first and second-generation adults, and molecular characterization of D2-D3 expansion segment of 28S rRNA region were given. Molecular phylogeny of the genus Steinernema was also provided. In addition, the study showed that the lethal efficacy of this local strain to larvae of Galleria mellonella L. was relatively higher than other reported EPN strains in Vietnam. Conclusions The Vietnamese EPN population found in this study was determined to be conspecific with S. surkhetense, revealed its new distribution in Vietnam. Besides, detailed morphological and molecular characterizations of it was provided with small variations compared to other populations in the world, and its relatively high lethal efficacy on larvae of G. mellonella implied that this strain can be potentially a useful strain for biological control of insect pests.

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Diversity of plant‐parasitic nematodes (PPNs) associated with medicinal plants in Vietnam, Vietnamese PPN checklist and a pictorial key for their identification

Nguyen,

Trinh,

Nguyen

et al. 2023

Plant Pathology

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Plant‐parasitic nematodes (PPNs) are one of the most damaging pests to plants and are able to cause significant damage to all parts of plants, including stems, leaves, flowers, fruits and roots. Studies on the diversity, host range, distribution and identification methods of PPNs are therefore vital in order to create a basis for management. This current study represents the first dedicated investigation of PPNs from medicinal plants in Vietnam, focusing on the diversity of nematodes associated with 23 different plant species. In combination with a literature review of PPNs in Vietnam, this work has resulted in an updated list of 217 PPN species belonging to 40 genera, 15 families and three orders and also provides a pictorial online key for the identification of 52 most common and important PPN genera of the world. This key is based on the most crucial diagnostic features of PPN females, including female body shape, cuticle, labial shape, cephalic framework, stylet, stylet base, pharynx, median bulb, pharyngeal gland, vulva, tail shape and phasmid. Pictorial representations of these genera and their diagnostic characters are included in the browser‐based key to benefit users from all levels in nematology, be they beginners or experts.

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First report of Xiphinema hunaniense Wang & Wu, 1992 (Nematoda: Longidoridae) in Vietnam

Cited by 3 publications

References 9 publications

First record of<i> Xiphinema hunaniense</i> Wang & Wu, 1992 (Dorylaimida: Longidoridae) associated with tea <i>(Camellia sinensis</i> (L.) Kuntze) in Thanh Hoa, Vietnam

First record of<i> Xiphinema hunaniense</i> Wang & Wu, 1992 (Dorylaimida: Longidoridae) associated with tea <i>(Camellia sinensis</i> (L.) Kuntze) in Thanh Hoa, Vietnam

First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam

Diversity of plant‐parasitic nematodes (PPNs) associated with medicinal plants in Vietnam, Vietnamese PPN checklist and a pictorial key for their identification

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