First Report of Isolation and Genome Sequence of L. petauri Strain from a Rainbow Trout Lactococcosis Outbreak

Kotzamanidis, Christos; Malousi, Andigoni; Bitchava, Konstantina; Vafeas, George; Chatzidimitriou, Dimitrios; Skoura, Lemonia; Papadimitriou, E; Chatzopoulou, Fani; Ζdragas, Αntonios

doi:10.1007/s00284-020-01905-8

“…The MLST analysis clustered 86 L. garvieae isolates into 32 distinct STs, with 5 CCs and 18 singletons, which is consistent with the population structure in isolates from other hosts ( 37, 38, 43 ), the environment, or foods ( 44 ). All strains, except Hebei-B-22, were new STs and phylogenetically close to each other.…”

Section: Discussionsupporting

confidence: 66%

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Lin

¹

,

Han

²

,

Barkema

³

et al. 2022

Preprint

View full text Add to dashboard Cite

Lactococcus garvieae is an emerging zoonotic pathogen, but there are few reports regarding bovine mastitis. The recent prevalence of L. garvieae poses an increasing disease threat and global public health risk. A total of 39 L. garvieae isolates were obtained from 2899 bovine clinical mastitis milk samples in 6 provinces of China from 2017 to 2021. Five clonal complexes were determined from 32 MLST types of L. garvieae; ST46 was the predominant sequence type and 13 novel MLST types were identified. All isolates were resistant to chloramphenicol and clindamycin, but susceptible to penicillin, ampicillin, amoxicillin-clavulanic acid, imipenem, ceftiofur, enrofloxacin, and marbofloxacin. Based on genomic analyses, L. garvieae had 6310 genes, including 1015, 3641 and 1654 core, accessory and unique genes. All isolates had virulence genes coding for collagenase, fibronectin-binding protein, Glyceraldehyde-3-phosphate dehydrogenase, superoxide dismutase and NADH oxidase. Most isolates had lsaD and mdtA AMR genes. Based on COG results, the functions of defense, transcription and replication, recombination and repair were enhanced in unique genes, whereas functions of translation, ribosomal structure and biogenesis were enhanced in core genes. The KEGG functional categories enriched in unique genes included human disease and membrane transport, whereas COG functional categories enriched in core genes included energy metabolism, nucleotide metabolism and translation. No gene was significantly associated with host specificity. In addition, core genome SNPs analysis suggested potential host adaptation of some isolates in several sequence types. Therefore, this study characterized L. garvieae isolated from mastitis and assessed host adaptation of L. garvieae to various hosts.

show abstract

“…Genomic analysis reassigned L. garvieae subgroup A strains to the recently described species L. petauri ( 3 ). Consequently, both species can be considered the etiological agents of lactococcosis in fish ( 4 , 5 ) and emerging opportunistic zoonotic pathogens ( 6 – 8 ). The isolation of both species from a variety of food ( 9 – 11 ) also implicates a contribution to the quality and typicity of various traditional food products ( 10 ).…”

Section: Announcementmentioning

confidence: 99%

Genome Sequences of Lactococcus garvieae and Lactococcus petauri Strains Isolated from Traditional Montenegrin Brine Cheeses

Martinović

¹

,

Rosel

²

,

Nisic

³

et al. 2021

Microbiol Resour Announc

View full text Add to dashboard Cite

show abstract

“…petauri LZys1, which have been analysed in this study, and other L. petauri strains such as CF11 [15] and LG_SAV_20 [16] allowed us to annotate the genome of L. petauri LZys1 and make further investigations.…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, probiotic associated genome analysis of multiple strains could reveal the evolutionary process and provide insights into genetic properties based on the genome map and sequences. The whole-genome sequences of L. petauri LZys1, which have been analysed in this study, and other L. petauri strains such as CF11 [15] and LG_SAV_20 [16] allowed us to annotate the genome of L. petauri LZys1 and make further investigations.…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Ouyang

¹

,

Zhang

²

,

Wang

³

et al. 2021

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. Lactococcus petauri LZys1 ( L. petauri LZys1) is a type of lactic acid bacteria (LAB), which was initially isolated from healthy human gut. Hypothesis/Gap Statement. It was previously anticipated that L. petauri LZys1 has potential characteristics of probiotic properties. The genetic structure and the regulation functions of L. petauri LZys1 need to be better revealed. Aim. The aim of this study was to detect the probiotic properties L. petauri LZys1 and to reveal the genome information related to its genetic adaptation and probiotic profiles. Methodology. Multiple in vitro experiments were carried out to evaluate its lactic acid-producing ability, resistance to pathogenic bacterial strains, auto-aggregation and co-aggregation ability, and so on. Additionally, complete genome sequencing, gene annotation, and probiotic associated gene analysis were performed. Results. The complete genome of L. petauri LZys1 comprised of 1 985 765 bp, with a DNA G+C content of 38.07 %, containing 50 tRNA, seven rRNA, and four sRNA. A total of 1931 genes were classified into six functional categories by Kyoto Encyclopaedia of Genes and Genomes (KEGG) database. The neighbour-joining phylogeny tree based on the whole genome of L. petauri LZys1 and other probiotics demonstrated that L. petauri LZys1 has a significant similarity to Lactococcus garvieae . The functional genes were detected to expound the molecular mechanism and biochemical processes of its potential probiotic properties, such as atpB gene. Conclusion. All the results described in this study, together with relevant information previously reported, made L. prtauri LZys1 a very interesting potential strain to be considered as a prominent candidate for probiotic use.

show abstract

First Report of Isolation and Genome Sequence of L. petauri Strain from a Rainbow Trout Lactococcosis Outbreak

Cited by 31 publications

References 28 publications

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Comparative genomic analyses of Lactococcus garvieae isolated from bovine mastitis in China

Genome Sequences of Lactococcus garvieae and Lactococcus petauri Strains Isolated from Traditional Montenegrin Brine Cheeses

Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut

Contact Info

Product

Resources

About