First report of seaweed-associated yeast from Indonesia:  Species composition and screening of their polysaccharides-degrading enzymes

Sibero, Mada Triandala; Frederick, E H; Sabdono, Agus; Wijayanti, Diah Permata; PRINGGENIES, DELIANIS; Radjasa, Ocky Karna; Zilda, Dewi Seswita; Murwani, Retno

doi:10.13057/biodiv/d230327

Cited by 4 publications

(7 citation statements)

References 80 publications

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“…Furthermore, Hodhod et al (2020) also informed about the antibacterial activity of crude extract of H. werneckii against Methicillin-resistant Staphylococcus aureus (MRSA), Campylobacter jejuni and Salmonella typhimurium. Interestingly, a marine H. werneckii SUCCY001 was isolated as a seaweed-associated marine yeast (Sibero et al 2022). This study also discovered the ability of H. werneckii SUCCY001 to degrade several marine polysaccharides.…”

Section: Introductionmentioning

confidence: 56%

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

et al. 2022

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Abstract. Fatmawati F, Sibero T, Trianto A, Wiyanti DP, Sabdono A, Pronggenies D, Radjasa OK. 2022. The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa. Biodiversitas 23: 5258-5266. Gracilaria verrrucosa is producer of agar, a polysaccharide, that widely used in pharmaceutical and food industries. However, several studies have shown that the secondary metabolite of this seaweed is not potential as an antimicrobial and antioxidant agents. This study aimed to determine the antibacterial and antioxidant properties of methanolic extract of G. verrucosa after fermentation using marine yeast Hortaea werneckii SUCCY001. The samples were fermented based on different optimization times, specially in three, six and nine days. Samples were extracted using methanol by maceration method. Then the extracts were tested for antimicrobial potential against the several human clinical pathogens namely Bacillus cereus, Bacillus subtilis, Escherichia coli, Micrococcus luteus and Pseudomonas aeruginosa. The antioxidant potential was analyzed using the 1,1-diphenyl-2-picrylhydracyl (DPPH) and 2,2-Azinobis 3-ethyl benzothiazoline 6- sulfonic acid (ABTS) method while total phenolic content (TPC) was measured using Folin-Ciocalteu. Secondary metabolites were characterized using thin layer chromatography (TLC) and GC-MS. TLC plate visualization using UV light, DPPH, FeCl3 and vanillin-sulfuric acid reagents. The results showed that the extract fermented for nine days was able to inhibit the growth of P. aeruginosa and M. luteus at a concentration of 2 mg/mL. The highest antioxidant activity was found in the nine days fermented extract with an RSA value of 21.9% ± 0,0001 for DPPH and 12.53 % ± 0.002 for ABTS. Furthermore, the highest total phenolic content was found in the nine days fermented extract of 7.57 GAE/mg. The results of characterization using TLC showed the presence of phenolic compounds, terpenoids and antioxidants in fresh seaweed extracts, autoclaved fresh seaweed, three days of fermentation, six days and nine days. Based on this research, it can be seen that the addition of marine yeast starter H. werneckii SUCCY001 was able to increase the antibacterial and antioxidant activity of G. verrucosa.

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Section: Introductionmentioning

confidence: 56%

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

et al. 2022

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“…Plenty of microorganism have been discovered from Panjang Island ecosystem, from prokaryotic like bacteria to eukaryotic microorganisms such as fungi [17][18][19] . Our previous study has succeeded in obtaining various species of association bacteria from this location such as Salinicoccus roseus, Sphingobium yanoikuyae, Streptomyces lateritius, Labrenzia marina, and Halomonas meridiana [20,21].…”

Section: Resultsmentioning

confidence: 99%

Characterization and identification of extracellular polysaccharides-degrading enzymes (epes)-producing marine sediment bacteria

Bondar,

Sibero,

Yudiati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The utilization of polysaccharide-degrading enzymes (EPEs) from bacteria has been increasing, therefore many studies are exploring new producers of EPEs. This study aimed to screen and identify the EPEs-producing marine sediment bacteria collected from Panjang Island, Jepara. A total of 11 bacteria were isolated from the sediment sample. Further, seven strains were selected to conduct further analysis, such as biochemical test and screening of enzyme activity. According to the calculation of enzyme activity index (EAI), it was noted that strain with given codes PP.K.15, PP.K.21, PP.K.6, and PP.K.20 demonstrated potential for carrageenase, alginate-lyase, amylase, and agarase. In addition, molecular identification using 16S rRNA gene sequencing discovered that PP.K.6 was identified as Basillus safensis, PP.K.11 as Sinomicrobium oceani, PP.K. 20 as Salinicola zeshunii, PP.K 15 and PP.K. 21 as Micrococcus luteus, and PP.K 22 as Qipengyuania flava.

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“…The selection of A. melanogenum MTGK.31 was based on our previous study which reported its ability in producing extracellular polysaccharides-degrading enzymes (EPEs) (Sibero et al, 2022). This yeast had a large colony size, filamentous shape and margin, convex elevation, smooth texture, and white cream color.…”

Section: Aureobasidium Melanogenum Mtgk31mentioning

confidence: 99%

“…However, most of them mainly focused on the lactic acid bacteria (LAB) and baker yeast Saccharomyces cerevisiae as the fermenting agents, while the utilization of marine yeast is rarely studied (Sharma et al, 2020). Hence, as the preliminary experiment, our previous study has reported the potential of seaweed-associated yeast Aureobasidium melanogenum MTGK.31 as one of the extracellular polysaccharides degrading enzymes (EPEs) producers (Sibero et al, 2022). Considering the outstanding result of microbial fermentation on seaweed biological properties in previous studies, in this work, we aimed to investigate the potential of marine yeast A. melanogenum MTGK.31 as the fermenting agent on Gracilaria verrucosa's biological properties and characterize the seaweed metabolites profile and antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Metabolites Alteration and Antioxidant Activity of Gracilaria verrucosa After Fermentation Using Aureobasidium melanogenum MTGK.31

Sibero

Savitri

Frederick³

et al. 2023

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Gracilaria verrucosa is a red seaweed that has been widely utilized in the food andpharmaceutical industries due to its biological properties. The utilization of biologicalagents in obtaining certain bioactive compounds would confront unavoidable issues,particularly its bioactive sustainability. Hence, microbial fermentation has been reported as a practical approach to maintaining bioactive production and boosting its properties. Our study aimed to evaluate the potential of marine yeast Aureobasidium melanogenum MTGK.31 as a fermenting agent for G. verrucosa and characterize the seaweed metabolite profile and antioxidant activity after fermentation. The seaweed was fermented using A. melanogenum MTGK.31 in a medium consisting of yeast extract, peptone, and glucose. The fermentation was done for 24, 48, and 72 hours. Total plate count and pH were measured after each fermentation period. The primary and secondary metabolites of G. verrucosa in each fermentation were observed. Antioxidant assay using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method was conducted, followed by total phenolic content using the Folin-Ciocalteu method. It was highlighted that yeast colony increased during the fermentation, while the pH level was decreasing. We found that the fermentation not only boosted some elements in primary metabolites, but also increased G. verrucosa bioactive groups. After 72 hours of fermentation, the G. verrucosa percent radical scavenging activity (%RSA) increased more than two times compared to the fresh G. verrucosa with a %RSA value of 16.09±6.57. Nevertheless, the highest total phenolic content of 5.62±0.00028 mg GAE/g extract was shown by G. verrucosa after 48 hours of fermentation.

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First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes

Cited by 4 publications

References 80 publications

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

Characterization and identification of extracellular polysaccharides-degrading enzymes (epes)-producing marine sediment bacteria

Metabolites Alteration and Antioxidant Activity of Gracilaria verrucosa After Fermentation Using Aureobasidium melanogenum MTGK.31

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