First Report of Slippery Skin Caused by Burkholderia gladioli in Stored Onion Bulbs in Mexico

Félix-Gastélum, Rubén; Maldonado-Mendoza, Ignacio E.; Olivas-Peraza, N. G.; Brito-Vega, Hortensia; Peñuelas-Rubio, Ofelda; Longoria-Espinoza, Rosa María

doi:10.1094/pdis-09-16-1370-pdn

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…After disease Predicted pathogenicity based on lesion of necrosis on tobacco leaves one week after injection. American Journal of Plant Sciences scoring, bacteria strains were re-isolated from the diseased tobacco plant to complete Koch's postulates [21].…”

Section: Virulence Evaluation With Tobacco Plantsmentioning

confidence: 99%

Characterization and in Plant Detection of Bacteria That Cause Bacterial Panicle Blight of Rice

Mulaw¹,

Wamishe²,

Jia³

2018

AJPS

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Burkholderia glumae presumably induces a grain rot symptom of rice that is threatening to rice production in most rice producing states of the USA. The present study was to identify the causal agent of bacteria panicle blight (BPB), virulence based on hypersensitive reactions and distribution of the pathogen within a plant. 178 rice panicles samples were analyzed with semi-selective media (CCNT), polymerase chain reaction (PCR) with bacterial DNA gyrase (gyrB) specific markers, and hypersensitive reactions on tobacco leaves. A total of 73 samples out of 178 produced a yellow bacterial colony with similar morphology on CCNT medium suggesting they were bacterial panicle diseases. However, with PCR reactions we only determined that 45 of 73 were due to B. glumae, and the causal agent for the remaining samples was undetermined. Within the 45 samples, 31 highly, 6 moderately, and 5 weakly virulent isolates were grouped based on lesion sizes of the hypersensitive reactions.Pathogenicity variability among the 45 B. glumae detected suggests that different degrees of pathogenicity exist. To determine the existence of bacteria in different plant tissues, naturally infected plant parts were examined with CCNT media and PCR analysis. B. glumae was again isolated from seeds followed by stems and sheaths from light yellow pigmented CCNT media. In contrast, roots and leaves show no visible yellow pigment on CCNT. Consistent PCR products were produced from the stem, sheath, and seed, but not from the root and leaves. These findings suggest that B. glumae is distributed in the stem, sheath, and seed, and not in the leaf and root. Together this study demonstrated the usefulness of artificial culture media, tobacco reactions, and DNA test with PCR for characterization of BPB, and distribution of bacteria in plants. These findings will help to understand the mechanism of bacteria translocation in plants.

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Section: Virulence Evaluation With Tobacco Plantsmentioning

confidence: 99%

Characterization and in Plant Detection of Bacteria That Cause Bacterial Panicle Blight of Rice

Mulaw¹,

Wamishe²,

Jia³

2018

AJPS

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“…Actualmente, se conoce muy poco de la ocurrencia de B. gladioli en México, y de las características fisiológicas y bioquímicas de este fitopatógeno. Solo existe una nota científica (Félix-Gastélum et al, 2017) en la que se reporta a B. gladioli como agente causal de pudrición en cebolla en Sinaloa, México.…”

Section: Fully Bilingualunclassified

“…Little is currently known on the occurrence of B. gladioli in Mexico or on the physiological and biochemical characteristics of this phytopathogen. There is only one scientific note (Félix-Gastélum et al, 2017) in which B. gladioli is reported as a causal agent of onion rotting in Sinaloa, Mexico.…”

Section: Fully Bilingualmentioning

confidence: 99%

Polyphasic characterization of Burkholderia gladioli isolated from onion and evaluation of its potential pathogenicity for other crops

Serret-López

Aranda-Ocampo

Espinosa-Victoria

et al. 2020

RMF

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Los individuos del género Burkholderia, sensu lato, se caracterizan por colonizar diversos nichos ecológicos. Esta investigación tuvo como objetivos caracterizar morfológica, fisiológica, bioquímica y molecularmente a Burkholderia gladioli, aislada de bulbos podridos de cebolla blanca (Allium cepa), y determinar su potencial como patógeno de otras especies vegetales. Burkholderia gladioli promovió, igualmente, la pudrición blanda en bulbos de cebolla morada (A. cepa), tallos de puerro (A. ampeloprasum), inflorescencias de brócoli (Brassica oleracea), cladodios de nopal (Opuntia indica), raíces de zanahoria (Daucus carota) y esporocarpos de champiñón (Agaricus bisporus). Burkholderia gladioli colonizó plantas de frijol (Phaseolus vulgaris) en invernadero y mostró resistencia natural in vitro a productos a base de cobre: cuprimicin agrícola, cuprimicin 500, phyton, oxicloruro de cobre; antibióticos: bactrol, bactriomicin agrícola, kasumin, final bacter, quartz y biológicos: serenade polvo, fungifree y bacterbest. Este estudio reporta por primera vez la caracterización polifásica de B. gladioli en México. Este aislado bacteriano creció a pH 4.0 y 42 °C, lo que podría conferirle habilidad competitiva en suelos ácidos y condiciones semiáridas. Se demostró que tiene un amplio rango de hospederos en condiciones postcosecha, además de ser resistente a diferentes productos usados en campo para el control de fitopatógenos.

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Burkholderia gladioli causes bacterial internal browning in sweetpotato of China

Zhang

Chen

Wang

et al. 2020

Australasian Plant Pathol.

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First Report of Slippery Skin Caused by Burkholderia gladioli in Stored Onion Bulbs in Mexico

Cited by 7 publications

References 2 publications

Characterization and in Plant Detection of Bacteria That Cause Bacterial Panicle Blight of Rice

Characterization and in Plant Detection of Bacteria That Cause Bacterial Panicle Blight of Rice

Polyphasic characterization of Burkholderia gladioli isolated from onion and evaluation of its potential pathogenicity for other crops

Burkholderia gladioli causes bacterial internal browning in sweetpotato of China

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