First report of Sweet potato leaf curl virus infecting sweet potato in Argentina

Pardina, Patricia Rodríguez; Luque, A. V.; Nome, Claudia; Colomba, E. López; Delgado, Soledad; Feo, L. Di

doi:10.1007/s13314-012-0073-7

Cited by 17 publications

(6 citation statements)

References 17 publications

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“…A phylogenetic tree was constructed with 29 begomovirus isolates, including three JCLCIV isolates from the present study. Sweet potato leaf curl virus was used to route the phylogram because it was reported to be a distinct group to the Tomato Yellow leaf curl virus (AJ971265 & AJ971524), which showed the highest similarity with the present isolates (Pardina et al, 2012). The phylogram showed no significant major grouping, but it formed multiple minor clades with three to four members in each clade.…”

Section: Genetic Similarity and Phylogenetics Of Jclcivmentioning

confidence: 99%

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Chauhan

Rahman

Mastan

et al. 2018

Gene

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Begomoviruses belong to the family Geminiviridae are associated with several disease symptoms, such as mosaic and leaf curling in Jatropha curcas. The molecular characterization of these viral strains will help in developing management strategies to control the disease. In this study, J. curcas that was infected with begomovirus and showed acute leaf curling symptoms were identified. DNA-A segment from pathogenic viral strain was isolated and sequenced. The sequenced genome was assembled and characterized in detail. The full-length DNA-A sequence was covered by primer walking. The genome sequence showed the general organization of DNA-A from begomovirus by the distribution of ORFs in both viral and anti-viral strands. The genome size ranged from 2844 bp-2852 bp. Three strains with minor nucleotide variations were identified, and a phylogenetic analysis was performed by comparing the DNA-A segments from other reported begomovirus isolates. The maximum sequence similarity was observed with Euphorbia yellow mosaic virus (FN435995). In the phylogenetic tree, no clustering was observed with previously reported begomovirus strains isolated from J. curcas host. The strains isolated in this study belong to new begomoviral strain that elicits symptoms of leaf curling in J. curcas. The results indicate that the probable origin of the strains is from Jatropha mosaic virus infecting J. gassypifolia. The strains isolated in this study are referred as Jatropha curcas leaf curl India virus (JCLCIV) based on the major symptoms exhibited by host J. curcas.

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Section: Genetic Similarity and Phylogenetics Of Jclcivmentioning

confidence: 99%

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Chauhan

Rahman

Mastan

et al. 2018

Gene

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“…Sweepoviruses, named for the sweet potato begomoviruses, are monopartite (no DNA-B, alpha-satellite, or beta-satellite components associated) and phylogenetically distinct from the Old and New World begomovirus groups ( Clark et al, 2012 ). Recently, increasing strains of whitefly transmitted sweepoviruses, mainly sweet potato leaf curl viruses, have been identified globally ( Trenado et al, 2011 ; Wasswa et al, 2011 ; Albuquerque et al, 2012 ; Bi and Zhang, 2012 ; Clark et al, 2012 ; Pardina et al, 2012 ; Liu et al, 2014 ). Besides sweet potato ( Ipomoea batatas ), sweepoviruses also infect other Ipomoea species worldwide, such as I. nil or I. setosa ( Trenado et al, 2011 ), and have been reported to cause substantial yield losses and cultivar decline regionally in some sweet potato varieties ( Clark and Hoy, 2006 ; Ling et al, 2010 ; Gibson and Kreuze, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis

Fan

Zhang

2017

Front. Plant Sci.

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Sweepoviruses have been identified globally and cause substantial yield losses and cultivar decline in sweet potato. This study aimed to investigate the interaction between sweepovirus and plant host by analyzing the function of the viral protein C4 of Sweet potato leaf curl virus-Jiangsu (SPLCV-JS), a sweepovirus cloned from diseased sweet potato plants in East China. Ectopic expression of the C4 in Arabidopsis altered plant development drastically with phenotypic changes including leaf curling, seedling twisting, deformation of floral tissues and reduction of pollen fertility, and seed number. Using bimolecular fluorescence complementation analysis, this study demonstrated that the SPLCV-JS C4 protein interacted with brassinosteroid-insensitive 2 (AtBIN2) in the plasma membrane of Nicotiana benthamiana cells. The C4 AtBIN2 interaction was further confirmed by yeast two-hybrid assays. This interaction led to the re-localization of AtBIN2-interacting proteins AtBES1/AtBZR1 into the nucleus which altered the expression of brassinosteroid (BR)-response genes, resulting in the activation of BR-signaling pathway. The interaction of SPLCV-JS C4 and AtBIN2 also led to the down-regulated expression of key genes involved in anther and pollen development, including SPROROCYTELESS/NOZZLE, DEFECTIVE IN TAPEL DEVELOPMENT AND FUNCTION 1, and ABORTED MICROSPORES, which caused abnormal tapetal development, followed by defective exine pattern formation of microspores and pollen release. Consequently, male fertility in the C4 transgenic Arabidopsis was reduced. The present study illustrated how the sweepovirus C4 protein functioned in host cells and affected male fertility by interacting with the key components of BR-signaling pathway.

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“…Las secuencias obtenidas a partir de los productos de PCR permitieron validar el protocolo de detección. Al comparar las mismas contra las bases de datos del GenBank la secuencia obtenida del SPLCV (100 %) con la secuencia completa reportada por Pardina et al (2012) para plantaciones en Argentina (accesión JQ349087), y la secuencia obtenida para el SPCSV coincide (100 %) con la accesión KU511274 del segmento Can181-9/AM-MB2. La secuencia obtenida para el SPFMV coincidió en un 98,9 % con la accesión MG656418 reportada por Maina et al (2018), lo que debe ser motivo de futuras investigaciones, ya que este potyvirus es reconocido por presentar variación genética que puede estar relacionada con la severidad de la infección y con la forma de interactuar con otros virus (Untiveros et al, 2008).…”

Section: Resultsunclassified

Detección de virus en camote (Ipomoea batatas L.) mediante qPCR

Varela‐Benavides

Trejos-Araya

2020

Agron. Mesoam.

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Introducción. El camote (Ipomoea batatas) es un cultivo muy susceptible a las enfermedades virales, las cuales se han asociado con una disminución del rendimiento del cultivo mayor al 50 %. En las plantaciones de camote costarricenses se ha reportado la presencia del virus del moteado plumoso (SPFMV), el virus del enanismo clorótico (SPCSV) y el virus del enrollamiento de la hoja (SPLCV); para el desarrollo de programas adecuados de control de estos virus, es necesaria la detección e identificación temprana mediante el uso de técnicas sensibles y eficientes. Objetivo. El objetivo de esta investigación fue estimar la incidencia del SPFMV, el SPCSV y el SPLCV en plantaciones de camote mediante qPCR. Materiales y métodos. En el 2018 se recolectaron muestras de tejido foliar de ochenta plantas con síntomas de infección viral en veinticinco plantaciones de camote distribuidas en cuatro provincias de Costa Rica. La detección de los tres virus se llevó a cabo mediante qPCR con el empleo de imprimadores y sondas de hidrólisis específicos. Resultados. En el 92 % de las plantaciones analizadas se encontró la presencia de virosis. La enfermedad viral del camote (SPVD), definida como la infección combinada del SPFMV y el SPCSV, fue la que se detectó con más frecuencia. Las plantaciones ubicadas en las localidades de Sabanilla, El Cacao y La Guácima de Alajuela fueron las únicas en presentar los tres virus estudiados. El virus SPLCV solamente fue detectado en las provincias de Limón y Alajuela. Conclusión. Es posible realizar una detección temprana de tres de los virus más frecuentes en camote con el empleo de qPCR, lo que contribuiría con los programas de producción de semilla certificada.

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First report of Sweet potato leaf curl virus infecting sweet potato in Argentina

Cited by 17 publications

References 17 publications

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

Isolation and sequence characterization of DNA-A genome of a new begomovirus strain associated with severe leaf curling symptoms of Jatropha curcas L.

C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis

Detección de virus en camote (Ipomoea batatas L.) mediante qPCR

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