First Report of the Carbapenem-Hydrolyzing Oxacillinase OXA-58 in
            <i>Acinetobacter baumannii</i>
            Isolates in Italy

Bertini, Alessia; Giordano, Alessandra; Varesi, Paola; Villa, Laura; Mancini, Carlo; Carattoli, Alessandra

doi:10.1128/aac.00166-06

Cited by 25 publications

(20 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Southern blot analysis with a bla OXA-58 -specific probe generated by PCR enabled us to localize this gene on the 40 kb plasmid. This plasmid was within the size range (10-150 kb) described by other authors (Bertini et al, 2006;Héritier et al, 2005;Marqué et al, 2005;Poirel et al, 2005;Wang et al, 2007).…”

Section: E Sevillano and Others 82mentioning

confidence: 77%

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Sevillano

Fernández

Bustamante

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

and the presence of carbapenemases as a mechanism of resistance to imipenem. Isolates were obtained from 46 patients (one isolate per patient; 30 males,16 females) with an age range of 1 day to 84 years, and were collected from different sample types, the majority from respiratory tract infections (17) and wounds (13). Resistance to imipenem was detected in 15 isolates collected from different hospitals of the city. These isolates grouped into the same genotype, named A, and were resistant to all antibiotics tested including imipenem, with susceptibility only to colistin. Experiments to detect carbapenemases revealed the presence of the OXA-58 carbapenemase. Further analysis revealed the location of the bla OXA-58 gene on a 40 kb plasmid. To our knowledge, this is the first report of carbapenem resistance in A. baumannii isolates from Bolivia that is conferred by the OXA-58 carbapenemase. The presence of this gene in a multidrug-resistant clone and its location within a plasmid is of great concern with regard to the spread of carbapenem-resistant A. baumannii in the hospital environment in Bolivia. INTRODUCTIONAcinetobacter baumannii is a nosocomial pathogen responsible for severe infections often diagnosed in patients attending hospitals worldwide. Carbapemens are considered the drugs of choice for treating these infections, but they are not always efficient due to the occurrence of multidrug-resistant strains of this bacterium (Canduela et al., 2006;Coelho et al., 2006;Higgins et al., 2010a;Mendes et al., 2009;Poirel et al., 2010). Mechanisms enabling the development of carbapenem resistance include an induced decrease in membrane permeability, efflux pump overexpression and the production of carbapenemases. Among these mechanisms, the production of carbapenemases plays a major role in carbapenem resistance in most Gram-negative bacilli, including A. baumannii clinical isolates (Canduela et al., 2006;Coelho et al., 2006;Poirel et al., 2010;Queenan & Bush, 2007;Walsh, 2010). The most common type of enzyme occurring in A. baumannii is represented by the carbapenem-hydrolysing class D b-lactamases, which are divided into five phylogenetic groups: OXA-23-like, OXA-24-like, OXA-58-like, OXA-51-like, which is intrinsic to A. baumannii, and the recently described OXA-143-like enzymes (Higgins et al., 2009;Queenan & Bush, 2007).Although A. baumannii is considered an emerging pathogen and much research has been carried out on it, there have been only a few studies concerning the mechanisms conferring carbapenem resistance of this bacterium in Latin American countries (Celenza et al., 2006;Coelho et al., 2006;Sader et al., 2004;Sgambatti et al., 2010). In addition, there are no previous publications regarding the resistance of A. baumannii to imipenem or the occurrence of carbapenemases in A. baumannii isolates in Bolivia.In this study, we analysed the antibiotic resistance, in particular to imipenem, of clinical isolates of A. baumannii obtained from several hospitals in Cochabamba, Bolivia, and the presence of carbape...

show abstract

Section: E Sevillano and Others 82mentioning

confidence: 77%

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Sevillano

Fernández

Bustamante

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…1). Two other acquired OXA-type gene clusters with carbapenemase activity have been described, including the bla OXA-24 -like (encoding OXA-24, -25, -26, and -40) (3,50,70,114,230,342,344) and the bla OXA-58 -like (36,42,98,196,421,440,441,445,550,564,617) carbapenemase genes. The crystal structure of OXA-24 was recently described and provides important insights for future drug development toward this emerging class of carbapenemases (477).…”

Section: ␤-Lactamsmentioning

confidence: 99%

Acinetobacter baumannii : Emergence of a Successful Pathogen

2008

View full text Add to dashboard Cite

SUMMARY Acinetobacter baumannii has emerged as a highly troublesome pathogen for many institutions globally. As a consequence of its immense ability to acquire or upregulate antibiotic drug resistance determinants, it has justifiably been propelled to the forefront of scientific attention. Apart from its predilection for the seriously ill within intensive care units, A. baumannii has more recently caused a range of infectious syndromes in military personnel injured in the Iraq and Afghanistan conflicts. This review details the significant advances that have been made in our understanding of this remarkable organism over the last 10 years, including current taxonomy and species identification, issues with susceptibility testing, mechanisms of antibiotic resistance, global epidemiology, clinical impact of infection, host-pathogen interactions, and infection control and therapeutic considerations.

show abstract

“…The rate of hydrolysis of imipenem was 10-fold lower, and that of meropenem was 100-fold lower, than that of benzylpenicillin (130). The bla OXA-58 gene was found to be plasmid borne in A. baumannii MAD (130), as well as in most of the OXA-58-producing A. baumannii isolates reported worldwide, including those in Europe, Argentina, Australia, and the United States (26-28, 69, 87, 117); OXA-58 is often associated with hospital outbreaks and has been involved in such outbreaks in France, Belgium, Italy, Turkey, Greece, and the United States (12,15,53,64,69,128,159). Noteworthily, OXA-58 has also been identified in other Acinetobacter species, such as A. junii in Romania (87) and Australia (117), Acinetobacter genomospecies 3 in Spain (88), and Acinetobacter phenon 6/ct13TU in Spain (89).…”

Section: Acquired Chdlsmentioning

confidence: 99%

Diversity, Epidemiology, and Genetics of Class D β-Lactamases

Poirel

Naas

Nordmann

2010

Antimicrob Agents Chemother

553

485

View full text Add to dashboard Cite

Class D ␤-lactamase-mediated resistance to ␤-lactams has been increasingly reported during the last decade. Those enzymes also known as oxacillinases or OXAs are widely distributed among Gram negatives. Genes encoding class D ␤-lactamases are known to be intrinsic in many Gram-negative rods, including Acinetobacter baumannii and Pseudomonas aeruginosa, but play a minor role in natural resistance phenotypes. The OXAs (ca. 150 variants reported so far) are characterized by an important genetic diversity and a great heterogeneity in terms of ␤-lactam hydrolysis spectrum. The acquired OXAs possess either a narrow spectrum or an expanded spectrum of hydrolysis, including carbapenems in several instances. Acquired class D ␤-lactamase genes are mostly associated to class 1 integron or to insertion sequences.

show abstract

First Report of the Carbapenem-Hydrolyzing Oxacillinase OXA-58 in Acinetobacter baumannii Isolates in Italy

Cited by 25 publications

References 7 publications

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Emergence and clonal dissemination of carbapenem-hydrolysing OXA-58-producing Acinetobacter baumannii isolates in Bolivia

Acinetobacter baumannii : Emergence of a Successful Pathogen

Diversity, Epidemiology, and Genetics of Class D β-Lactamases

Contact Info

Product

Resources

About