First report of the effect of Ocotea elegans essential oil on Rhipicephalus (Boophilus) microplus

Abstract

Rhipicephalus (Boophilus) microplus is responsible for reducing animal welfare, causing a drop in productive performance and transmitting hemoparasites. The main strategy of tick control is application of synthetic acaricides. However, parasite resistance to these compounds is a major concern. Therefore, the acaricidal and repellent in vitro effect of the Ocotea elegans essential oil on larvae and adult females of R. (B.) microplus were evaluated. The larval packet test (LPT), larval repellency test (RT) and adult immersion test (AIT) were performed. The essential oil was analyzed by gas chromatography (GC/FID) and the structure of the oil's major constituent (92.2% sesquirosefuran) was elucidated by nuclear magnetic resonance. In the AIT, efficacy higher than 90% was detected from the concentration 25 mg/mL upward. In both LPTs performed after 48 h, only the 100 mg/mL concentration resulted in mortalities above 70%. On the other hand, the essential oil caused an average of 95.8% repellency from 0.78 to 100 mg/mL. The LC in the two LPT (48 h) tests were 59.68 and 25.59 mg/mL, respectively. The LC and LC in the AIT were 4.96 and 17.37 mg/mL, and in the RT they were 0.04 and 1.24 mg/mL respectively. We conclude that the essential oil of O. elegans leaves has a significant acaricidal effect on engorged females and on larval repellency of R. (B.) microplus ticks, and can be a promising alternative for the control of this ectoparasite.

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC‐DESI‐MSn

“…Species of this genus are known for producing a variety of secondary metabolites, with neolignans, aporphine and benzylisoquinoline‐type alkaloids as the most representative classes 2,3 . A wide range of biological activities from Ocotea species has been described in the literature, including acaricide, 4,5 antiproliferative, 6 anti‐inflammatory 7 and the inhibition of acetylcholinesterase (AChE) 8 …”

Plant-Derived Natural Compounds for Tick Pest Control in Livestock and Wildlife: Pragmatism or Utopia?

“…Treatment with essential oils from some plants can affect tick mortality, fecundity, and egg hatching rate [ 122 ]. Recent studies examined the potential use of essential oils of Laurus nobilis (laurel) [ 162 ], Ocotea odorifera (canela sassafras) [ 163 ], Chrysopogon zizanioides [ 164 ], and Schinus molle [ 165 ] with levels of control between 7.59% to 99%, depending on the concentration of essential oil, tick life stage, and species. The combination of essential oils appears to enhance the effect of individual oils [ 166 , 167 ].…”

Chemical Diversity and Biological Activities of Essential Oils from Licaria, Nectrandra and Ocotea Species (Lauraceae) with Occurrence in Brazilian Biomes

“…β-Eudesmol (27.51%) was the main compound in the stems, and the oil of roots showed elemol (24.31%) and β-eudesmol (13.44%) [ 38 ]. Conversely, the leaf oil of Ocotea elegans Mez collected in Carapebus (RJ) displayed a high amount of sesquirosefuran (92.20%) [ 51 ].…”

“…Leaves of O. odorifera from Machado (MG) were mainly constituted of safrole (36.30%), γ-cadinene (6.60%) and camphor (6.50%). The EO toxicological effect was evaluated in peritoneal macrophages of BALB/c mice and exhibited CC 50 49.52 µg/mL in comparison to the positive control amphotericin B with CC 50 51.86 µg/mL [55]. The leaf EOs of some Ocotea species were also tested by the brine shrimp lethality assay.…”

“…Cytotoxic, fibroblast cells (NIH/3T3, IC50 58.0 µg/mL); sarcoma cells (J774.A1, IC50 29.40 µg/mL) IC 50 2.70 µg/mL; L. amazonensis, amastigotes, IC 50 2.10 µg/mL). Cytotoxic, fibroblast cells (NIH/3T3, IC50 51.60 µg/mL); sarcoma cells (J774A.1, IC50 29.90 µg/mL)…”