First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil

Müller, Cristiane; Esteves, Mariana Bossi; Kleina, Heloisa Thomazi; Nondillo, Aline; Botton, Marcos; Lopes, João Roberto Spotti

doi:10.1007/s40858-021-00430-8

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…Greenhouse experiments (season 2020) were conducted to test the acquisition and inoculation of X. albilineans by M. fimbriolata in plant probing assays. Experiments were performed based on previous insect vector competence studies ( Lenzi et al 2019 , Esteves et al 2019 , 2020 , Müller et al 2021 ). For this, 3-month-old healthy sugarcane plants were inoculated according to the decapitation method at 30 cm from the soil ( Rott et al 1997 , Brumbley et al 2004 ), using 50 μl of Xa11 inoculum suspended in PBS (10 8 CFU/ml).…”

Section: Methodsmentioning

confidence: 99%

Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald

Bini,

Serra,

Pastore

et al. 2023

Journal of Insect Science

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Leaf scald is a destructive sugarcane disease caused by the bacterium Xanthomonas albilineans (Ashby) Dowson. This pathogen presents the gene cluster SPI-1 T3SS, a conserved feature in pathogens vectored by animals. In this study, the competence of Mahanarva fimbriolata (Stål), a spittlebug commonly found in sugarcane fields in Brazil, was evaluated for the transmission of X. albilineans. Artificial probing assays were conducted to investigate the ability of M. fimbriolata adults to acquire X. albilineans from artificial diets containing the pathogen with subsequent inoculation of X. albilineans into pathogen-free diets. Plant probing assays with M. fimbriolata adults were conducted to evaluate the acquisition of X. albilineans from diseased source plants and subsequent inoculation of healthy recipient sugarcane plants. The presence of X. albilineans DNA in saliva/diet mixtures of the artificial probing assays and both insects and plants of the plant probing assays were checked using TaqMan assays. The artificial probing assays showed that M. fimbriolata adults were able to acquire and inoculate X. albilineans in diets. Plant probing assays confirmed the competence of M. fimbriolata to transmit X. albilineans to sugarcane. Over the entire experiment, 42% of the insects had acquired the pathogen and successful inoculation of the pathogen occurred in 18% of the recipient-susceptible sugarcane plants at 72 or 96 h of inoculation access period. Assays evidenced the vector competence of M. fimbriolata for transmission of X. albilineans, opening new pathways for investigating the biology and the economic impacts of the interaction between X. albilineans and M. fimbriolata.

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Section: Methodsmentioning

confidence: 99%

Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald

Bini,

Serra,

Pastore

et al. 2023

Journal of Insect Science

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“…The bacterium adheres and multiplies in the anterior part of the oral system (stomodeum), which presents physical, morphological and nutritional characteristics similar to those of xylem vessels (PURCELL, 1979;JANSE;OBRADOVIC, 2010). Among leafhopper species transmitting the disease commonly found in orchards in southern and southeastern Brazil, Macugonalia cavifrons (Stål), Macugonalia leucomelas (Walker) and Sibovia sagata (Signoret) stand out (MÜLLER et al, 2021). PLS dissemination also occurs through propagation material, such as grafting carried out from infected material and contaminated seedlings (DUCROQUET et al, 2001;AZEVEDO FILHO et al, 2011).…”

Section: Transmission / Disseminationmentioning

confidence: 99%

“…Aspects related to the insect-plant interaction can also influence the transmission efficiency. The leafhopper species, the feeding site on the plant, the ingestion rate and the soil water conditions can also influence the survival and feeding of the leafhopper on the host plant (PEREIRA et al, 2005;MÜLLER et al, 2021). As the distribution of X. fastidiosa is uneven in the plant, the feeding site of the vector is one of the main factors that can influence the probability of acquisition or inoculation of the pathogen (MIRANDA et al, 2008).…”

Section: Transmission / Disseminationmentioning

confidence: 99%

“…There is no well-established chemical management recommended to control PLS. Therefore, all efforts should be directed towards preventing its introduction into new areas, highlighting the acquisition of healthy seedlings, field monitoring of diseased plants and vectors, eradication of plant material with symptoms, among others, in order to avoid the establishment of orchards close to infected areas and the planting of disease-resistant cultivars (DUCROQUET et al, 2001;MÜLLER et al, 2021).…”

Section: Control and Prevention Measuresmentioning

confidence: 99%

“…PLS may make the economic plum exploitation in Brazil unfeasible. It has been the main factor for the crop decline in the southern and southeastern states of the country (MAY DE MIO; GARRIDO; UENO, 2004;MÜLLER et al, 2021), in addition to causing the early abandonment of orchards. There is little information about the management and control of the disease.…”

Section: Introductionmentioning

confidence: 99%

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Plum leaf scald: characteristics of the causative agent,symptoms, dissemination, control and prevention

Gabardo

Silva

2021

Rev. Bras. Frutic.

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The very slow-growing gram-negative bacterium, Xylella fastidiosa, inhabits the xylem and is vector-borne. It causes several diseases in plants, including plum leaf scald (PLS), which is the main limiting factor over the years for the crop expansion, both in productivity and in cultivation areas in Brazil. There is little information about the management and control of the disease. Brazilian breeding programs of plum trees have launched few resistant cultivars. The aim of this work was to carry out a systematic review with current information regarding PLS, characteristics of the causative agent, symptoms, transmission/dissemination, control and prevention. The use of certified propagation material, free from bacteria and the elimination of infected plants are the main measures used in Brazil. The vast majority of commercial cultivars are highly susceptible to bacteria and only few cultivars of interest have any resistance. In 2017 the Agricultural Research and Rural Extension Company of Santa Catarina launched the ‘Zafira’ plum cultivar, the first cultivar for commercial purposes that is not naturally infected; however, it was observed that the transmission of the bacteria occurs by grafting. The Institute of Rural Development of Paraná IAPAR-EMATER evaluated different plum crosses and concluded that the ‘PR-1095’ genotype was the most resistant and did not show any foliar symptoms of the disease. However, the PCR test revealed the presence of the bacteria, indicating that the genotype is probably tolerant. In contrast, ‘PR 1142’, ‘PR 1149’ and ‘PR 1260’ genotypes do not show symptoms or the presence of the bacteria by PCR, resulting in resistance to the disease. These genotypes have not been released, and further studies are still needed.

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Transmission of Xylella fastidiosa subspecies multiplex from naturally infected to healthy Rhamnus alaternus by Philaenus spumarius and Neophilaenus campestris

Nencioni,

Gargani,

Strangi

et al. 2024

J Pest Sci

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In Europe, the recently reported plant pathogen Xylella fastidiosa subsp. multiplex affects several wild, ornamental, and cultivated trees causing scorch diseases. In 2018, the sequence type 87 was reported in Tuscany on Mediterranean shrubs and trees. Although spittlebugs (Hemiptera: Aphrophoridae) were already identified as main vectors of this bacterium in Europe, their role in the transmission of this subspecies has not been ascertained yet. In this study the ability of Philaenus spumarius and Neophilaenus campestris to acquire and transmit Xylella fastidiosa subsp. multiplex sequence type 87 from and to Rhamnus alaternus was evaluated in two-year semi-field experiments. To acquire the bacterium, insects were confined on wild, naturally infected R. alaternus shrubs for 120 h. Then, they were transferred to healthy plants and maintained in cages for 96 h. To follow the infection, plant samples were collected every two months for three times. Tested plants were destroyed at the end of experiments and roots, twigs and leaves were analysed. Philaenus spumarius showed a significantly higher survival rate than N. campestris. The infection status of both insects and plants was assessed through molecular analysis. P. spumarius and N. campestris were able to infect healthy plants although the acquisition rate and the estimated probability of transmission appeared to be low. These findings provide new accounts on the role of two polyphagous insect vectors in spreading a quarantine organism, which is lethal to a huge number of plant species. However, further studies are needed to disclose more specific interactions within this complex pathosystem.

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First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil

Cited by 11 publications

References 33 publications

Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald

Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald

Plum leaf scald: characteristics of the causative agent,symptoms, dissemination, control and prevention

Transmission of Xylella fastidiosa subspecies multiplex from naturally infected to healthy Rhamnus alaternus by Philaenus spumarius and Neophilaenus campestris

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