Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells

Bouwens, Mark; Rest, Ondine van de; Dellschaft, Neele; Bromhaar, Mechteld Grootte; Groot, Lisette C. P. G. M. de; Geleijnse, Johanna M.; Müller, Michael; Afman, Lydia A.

doi:10.3945/ajcn.2009.27680

Cited by 290 publications

(272 citation statements)

References 41 publications

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“…Interestingly, when we incubated PBMC ex vivo with the fatty acids ALA or EPA, the same pattern with respect to the inflammatory response was observed, with EPA having the most prominent inflammatory potential compared with ALA. Anti-inflammatory effects on circulating markers have been observed in studies among healthy individuals after intake of fish oil supplements even though many studies also show no effect (27,28) . However, little is known about the postprandial or long-term effect of intake of fish oil on the expression of inflammatory genes in PBMC (22,29) . It has previously been shown that the n-3 fatty acid ALA has antiinflammatory activity (28) and inhibits expression and secretion of IL-6 from cultured human monocytes (30) .…”

Section: Discussionmentioning

confidence: 99%

Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women

et al. 2011

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The aim of the present study was to examine the effect of a single high-fat meal with different fat quality on circulating inflammatory markers and gene expression in peripheral blood mononuclear cells (PBMC) to elucidate the role of fat quality on postprandial inflammation. A postprandial study with fourteen healthy females consuming three test meals with different fat quality was performed. Test days were separated by 2 weeks. Fasting and postprandial blood samples at 3 and 6 h after intake were analysed. The test meal consisted of three cakes enriched with coconut fat (43 % energy as saturated fat and 1 % energy as a-linolenic acid (ALA)), linseed oil (14 % energy as ALA and 30 % energy as saturated fat) and cod liver oil (5 % energy as EPA and DHA and 5 % energy as ALA in addition to 31 % energy as saturated fat). In addition, ex vivo PBMC experiments were performed in eight healthy subjects investigating the effects of EPA and ALA on release and gene expression of inflammatory markers. The IL-8 mRNA level was significantly increased after intake of the cod liver oil cake at 6 h compared with fasting level, which was significantly different from the effect observed after the intake of linseed cake. In contrast, no effect was seen on circulating level of IL-8. In addition, ALA and EPA were shown to elicit different effects on the release and mRNA expression levels of inflammatory markers in PBMC cultured ex vivo, with EPA having the most prominent proinflammatory potential.

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Section: Discussionmentioning

confidence: 99%

Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women

et al. 2011

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“…The use of these cells in nutrition-related studies is increasing due to the number of different studies performed in both animals and humans that have demonstrated the relationship of PBMCs-captured metabolic changes with the fasting and feeding cycles (Bouwens et al 2007;Caimari et al 2010a, b;Oliver et al 2013) and with the intake of specific nutrients or bioactive food compounds such as fatty acids, proteins, carbohydrates and polyphenols (van Erk et al 2006;Crujeiras et al 2008;Bouwens et al 2009;Konstantinidou et al 2009;Khymenets et al 2009;Bouwens et al 2010;Camargo et al 2010;Rudkowska et al 2011;Van Dijk et al 2012a, b;Tomé-Carneiro et al 2013;van der Velpen et al 2013; Electronic supplementary material The online version of this article (doi:10.1007/s12263-014-0438-y) contains supplementary material, which is available to authorized users. Afman et al 2014).…”

Section: Introductionmentioning

confidence: 99%

The intake of a high-fat diet and grape seed procyanidins induces gene expression changes in peripheral blood mononuclear cells of hamsters: capturing alterations in lipid and cholesterol metabolisms

et al. 2014

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We previously demonstrated that hamsters that were fed either a standard diet (STD) or a high-fat diet (HFD) and treated with a grape seed procyanidin extract (GSPE) showed decreased adiposity and circulating levels of free fatty acids compared with hamsters treated with a vehicle (Caimari et al. in Int J Obes 37:576-83, 2013, doi:10.1038/ijo.2012). Here, we tested whether the gene expression changes in peripheral blood mononuclear cells (PBMCs) can reflect these metabolic effects and the dyslipidaemia produced by the HFD feeding in the same cohort of animals. The mRNA levels of a subset of genes were also studied in the liver in order to evaluate the capacity of PBMCs to reflect the metabolic adaptations that occur in this organ. In PBMCs, we reported a simultaneous up-regulation of the lipid-related genes involved in both the anabolic (pparc, acc1 and gpat) and the catabolic (ppara, ucp2, atgl and hsl) pathways in response to the GSPE treatment, similar but no identical to previous observations in retroperitoneal white adipose tissues of these animals. Furthermore, the key cholesterol metabolism genes srebp2 and ldlr were significantly down-regulated in PBMCs of both HFD-fed groups compared with the STD groups. Although the expression of srebp2 in the liver followed a similar pattern to that obtained in PBMCs, no comparable changes were found between the liver and PBMCs in the expression of most of the studied genes. In conclusion, our results highlight the potential of PBMCs as a high accessible tissue for the indirect study of cholesterol and adipose tissue metabolism dynamics.

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“…The suitability of leukocytes for gene profiling is related to their active metabolism [22] and accessibility by simple venipuncture [21,23], which may allow a repeated time-series analysis of changes in gene expression in response to changing environmental or disease factors. Previous studies have demonstrated the potential of using gene expression levels of peripheral blood leukocytes as a novel biomarker of nutritional interventions [24], to predict weight loss regain in obese subjects [25,26], and to predict the inflammatory response to functional foods [27].…”

Section: Discussionmentioning

confidence: 99%

Osteochondrosis-Related Gene Expression in Equine Leukocytes Differs among Affected Joints in Foals

Serteyn

Piquemal²,

Mendoza

et al. 2014

J Mol Biomark Diagn

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Osteochondrosis (OC) is a developmental disease in horses with a significant impact on the horse's welfare and performance. Previously, differentially expressed genes in leukocytes of OC-affected have been identified and were differentially expressed in horses of different ages when compared to their age-matched controls.As the time course of the development of OC lesions seems to be joint dependent,the aim of this study is to compare in young OCaffected horses (between 8 to 12 months), the different expression of selected genes depending the joints involved.The expression of OC-related genes were analysed by rt-PCR and subsequent statistical analysis (ΔΔCT) in the leukocytes of 30 Belgian Warmblood horses aged between 8 to 12 months divided in groups depending the affected joints (fetlock, hock and stifle).In the three groups, expression of ApoB-3G, MGAT4A, B4GALT6 and PRKCG genes were significantly higher in the OC-affected foals compared to the healthy foals. Based on the profiles of expression ofApoB-3G, Dsh1/Dvl1, Foxl1, Hp, ISG15, Mark2, PPR2A, RUSC2 and WASH1 genes,the localization of the disease can be determined: expression levels of ApoB3G, WASH1 and FOXl1 to identify fetlock, ApoB3G, PPR2A to identify OC-development in the hock and ApoB3G, Dsh1/Dvl1, WASH1, PPP2R1A and Mark2 geneto identify OC-development in the stifle. However at this moment, the rt-PCR analysis of the identified genes as biomarkers gives only diagnostic information. For the future, the profile of expression of these genes could give also some predictive information on the evolution of the disease such as remission or permanent OC-lesions.

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Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells

Abstract: These results are the first to show that intake of EPA+DHA for 26 wk can alter the gene expression profiles of PBMCs to a more antiinflammatory and antiatherogenic status. This trial was registered at clinicaltrials.gov as NCT00124852.

Cited by 290 publications

References 41 publications

Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women

Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women

The intake of a high-fat diet and grape seed procyanidins induces gene expression changes in peripheral blood mononuclear cells of hamsters: capturing alterations in lipid and cholesterol metabolisms

Osteochondrosis-Related Gene Expression in Equine Leukocytes Differs among Affected Joints in Foals

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