Fish scale derived collagen/ginsenoside Rb1 biocomposites: preparation, characterization and their hemostatic ability

Abstract

This paper reports the characteristics and hemostatic ability of the collagen/ginsenoside Rb1 composites with different ginsenoside Rb1 content, in which, collagen was extracted from fresh water fish scales and ginsenoside Rb1 was extracted from Panax notoginseng. Glucose and glutaraldehyde were used as crosslinking agents for collagen molecules. Infrared (IR) spectroscopy and Field emission scanning electron microscopy (FESEM) were applied to assess the functional groups, interactions and morphology of the collagen/ginsenoside Rb1 composites. Glutaraldehyde exhibits a positive effect on improvement the dispersion of ginsenoside Rb1 in collagen matrix as well as on the interactions between ginsenoside Rb1 and collagen. In addition, the biocompatibility of the collagen/ginsenoside Rb1 in simulated body fluid was evaluated by ultraviolet-visible spectroscopy (UV-Vis). The obtained result shows that ginsenoside Rb1 can release well from the composite containing 1 wt.% of ginsenoside Rb1. Besides, the hemostatic ability of the composites was also tested and discussed.

Characterizations and bioactivities of novel biocomposites based on modified fish collagen, ginsenoside Rb1, and Camellia chrysantha polyphenols

“…In our previous report, 35 the 3D printed collagen film has a coagulation time of 3.77 min while collagen prepared by solution method has a coagulation time of 3.45 min. 16 The biomaterial samples formed using the 3D printing approach exhibited superior hemostatic performance compared to those prepared using the solution method and they exhibit the hemostatic ability better than collagen films. Therefore, some characteristics of 3D printed collagen-based biomaterials were taken into account below.…”

“…For collagen/1 wt.% ginsenoside Rb1 film prepared by solution method, the content of ginsenoside Rb1 released reached 17.13% and 21.49% after 5 and 10 min of testing in the SBF, respectively. 16 In the presence of polyphenols, the ginsenoside Rb1 released from the S3-SM film was continued with a content increased from 1.50% to 18.33% after 5-90 min of contact. This is an advantage of this film in this study due to the gradual release of ginsenoside Rb1 in SBF could give a better treatment.…”

“…12 Ginseng plants, because of their high bioactive value, are grown extensively. [13][14][15][16] The primary bioactive components of ginseng-ginsenosides, exhibit a range of pharmacological actions, including anti-inflammatory and antioxidant, 17,18 reducing neurological damage, 19 antidiabetic, 20 and antitumor 21 properties. Ginsenoside Rb1 makes up around 20% of the overall ginsenoside composition.…”

A ternary biocomposite based on modified fish scale collagen and ginsenoside Rb1: preparation, properties and bioactivities

“…Ginsenoside Rb1 (extracted from Panax pseudoginseng ) was added to the modified collagen matrix to improve the bioactivities of collagen samples because ginsenoside Rb1 in Radix ginseng has been shown to improve blood circulation and hemostasis and to treat sepsis by Yuan et al 21 . In our previous work, when adding 1 wt% of ginsenoside Rb1 into a collagen matrix, the hemostatic ability of the collagen was improved 22 . The characteristics, biocompatibility, anti‐inflammatory and antimicrobial activities as well as in vitro hemostatic ability of these films were assessed.…”