This study reports the suitable condition to extract hydroxyapatite (HAp) and collagen from tilapia scales in North Viet Nam. The tilapia scales were treated by Ca(OH)2 5 % solution for 24 hours to remove protein, fat and impurities. The protein-free scales were continuously sunk in mixture acid solutions to remove minerals. The HAp was obtained from this solution by precipitating with 0.5 M NaOH. The mineral-free scales were immersed in 0.5 M CH3COOH solution and precipitated collagen was obtained by adding of 0.5 M NaCl solution. The methods including Energy dispersive X-ray diffraction (EDX), Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM) were used to determine characteristics, structure and morphology of HAp and collagen. The HAp was dried at 150 o C for removing water and some organic substances and then, washed repeatedly by distilled water to obtain high purity HAp, size of 30-40 nm, and Ca/P ratio = 1.70, closed to Ca/P ratio = 1.67 in bones and human teeth. The collagen was obtained in raw form, contains C, N, O, S, Na, Cl atoms in structure.
This paper focuses to study on change of some characteristics of high density polyethylene (HDPE) pipes testing in Nghe An province seawater. The investigated characteristics of HDPE pipe before and after testing are structural, morphological, crystal, thermal property and stability. Infrared (IR) spectroscopy, scanning electron microscope (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and differential thermal analysis (DTA) methods were used to evaluate the change in characteristics of PE pipes after 10 testing months in Nghe An province seawater. In addition, the change in weight and tensile properties of PE pipes after testing was also investigated. The obtained results showed that the Nghe An province seawater had an influence on the structural, morphological, tensile, crystal and thermal characteristics of PE pipes.
This paper presents some characteristics, morphologies and bioactivities of modified collagen films (MCF) in a combination of fish scale collagen gel, crosslinking agent and ginsenoside Rb1. Collagen was extracted from freshwater fish scales by biochemical methods using pepsin enzyme to improve collagen hydrolysis. Glutaraldehyde, glucose and riboflavin were chosen as modification agents for the obtained collagen. These collagen samples were processed into MCF by a 3D printing method. The characteristics and morphologies of MCF were evaluated using infrared, energy‐dispersive X‐ray, field emission scanning electron microscopy and high‐performance liquid chromatography techniques. Moreover, the biocompatibility, hemostatic ability and anti‐inflammatory and antimicrobial activities of MCF were tested for preliminarily assessing the application efficiency of MCF. The obtained results suggest that crosslinking agent and ginsenoside Rb1 have effects on properties of collagen. MCF are well biocompatible, have great anti‐inflammatory and antimicrobial ability, are nontoxic to normal cells and have short blood clotting time. They are promising for applications of 3D printed modified collagen/ginsenoside Rb1 biocomposite films in biomedicine as hemostatic agents or other biomedical materials. © 2022 Society of Industrial Chemistry.
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