Fish species identification in canned tuna by PCR-SSCP: validation by a collaborative study and investigation of intra-species variability of the DNA-patterns

Rehbein, Hartmut; Mackie, I. M.; Pryde, Susan E.; Gonzales-Sotelo, Carmen; Medina, Isabel; Pérez‐Martín, Ricardo I.; Quinteiro, Javier; Rey-Méndez, Manuel

doi:10.1016/s0308-8146(98)00125-3

Cited by 82 publications

(59 citation statements)

References 13 publications

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“…[7,8] Nevertheless, fragment size is a limiting factor for the subsequent PCR reaction, which is based on the selective amplification of specific regions of DNA using oligonucleotides. [8] PCR [9][10][11] and its modifications-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), [12][13][14][15] Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), [16,17] real-time PCR, [18][19][20][21] and Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) [22] -represent crucial approaches for tuna fish species identification. These approaches comprise DNA extraction from the sample, PCR, and electrophoresis, or alternatively other detection systems for the evaluation of the final results.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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The most common techniques used to identify tuna species include methods based on the detection of specific DNA. The quality of species-specific DNA crucially affects the efficiency of amplification during the subsequent polymerase chain reaction (PCR). Detection of DNA in processed products can be adversely affected by DNA fragmentation during the processing steps and the use of ingredients that may inhibit the PCR reaction. In this study, several processing treatments applied to the muscle tissue of yellowfin tuna (Thunnus albacares) were evaluated. For DNA isolation three DNA extraction methods were compared. The concentration, purity, and amplificability of DNA were tested. The results revealed the variability among extraction procedures in terms of DNA quality and quantity in tuna muscle tissue processed under different processing technologies. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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“…Furthermore, it is possible to analyze degraded DNA and small fragments (Rehbein et al, 1999). This technique may also be used for preliminary screening before sequencing, as completed by several authors in the molecular identification of species (Hodgkinson et al, 2002;MacGregor and Amann, 2006;Mohr and Tebbe, 2006).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of Amazonian stingless bees using polymerase chain reaction single-strand conformation polymorphism

Júnior

Carvalho‐Zilse

2014

Genet. Mol. Res.

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ABSTRACT. In countries containing a mega diversity of wildlife, such as Brazil, identifying and characterizing biological diversity is a continuous process for the scientific community, even in face of technological and scientific advances. This activity demands initiatives for the taxonomic identification of highly diverse groups, such as stingless bees, including molecular analysis strategies. This type of bee is distributed in all of the Brazilian states, with the highest species diversity being found in the State of Amazônia. However, the estimated number of species diverges among taxonomists. These bees are considered the main pollinators in the Amazon rainforest, in which they obtain food and shelter; however, their persistence is constantly threatened by deforestation pressure. Hence, it is important to classify the number and abundance of bee specie, to measure their decline and implement meaningful, priority conservation strategies. This study aims to maximize the implementation of more direct, economic and successful techniques for the taxonomic identification of stingless bees. Specifically, the genes 16S rRNA and COI from mitochondrial DNA were used as molecular markers to differentiate 9 species of Amazonian stingless bees based on DNA polymorphism, using the polymerase chain reaction-single-strand conformation polymorphism technique. We registered different, exclusive SSCP haplotypes for both genes in all species analyzed. These results demonstrate that SSCP is a simple and cost-effective technique that is applicable to the molecular identification of stingless bee species.

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“…The presence of species-specific polymorphisms due to the several mutations normally occurring in the genome [103] has encouraged researchers to develop a variety of DNA-based methods for fish species identification. Some methods include the use of PCR together with the restriction fragment length polymorphism (RFLP) [9], forensic information nucleotide sequencing (FINS) [10], polymorphism of the length of the amplified fragment (AFLP) [11], or single-strand conformational polymorphism (SSCP) [12,13]. These techniques have been applied to the identification of numerous species of fish and seafood, including gadoids [14], flatfish [15,16], salmonids [11,104], scombroids [105,106], sardines and anchovies [107,108], eels [109], and mollusks [110,111].…”

Section: Biological Methodsmentioning

confidence: 99%

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

Fiorino

Garino

Arlorio

et al. 2018

Journal of Food Quality

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Authenticity and traceability of food products are of primary importance at all levels of the production process, from raw materials to finished products. Authentication is also a key aspect for accurate labeling of food, which is required to help consumers in selecting appropriate types of food products. With the aim of guaranteeing the authenticity of foods, various methodological approaches have been devised over the past years, mainly based on either targeted or untargeted analyses. In this review, a brief overview of current analytical methods tailored to authenticity studies, with special regard to fishery products, is provided. Focus is placed on untargeted methods that are attracting the interest of the analytical community thanks to their rapidity and high throughput; such methods enable a fast collection of "fingerprinting signals" referred to each authentic food, subsequently stored into large database for the construction of specific information repositories. In the present case, methods capable of detecting fish adulteration/substitution and involving sensory, physicochemical, DNA-based, chromatographic, and spectroscopic measurements, combined with chemometric tools, are illustrated and commented on.

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Fish species identification in canned tuna by PCR-SSCP: validation by a collaborative study and investigation of intra-species variability of the DNA-patterns

Cited by 82 publications

References 13 publications

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Molecular identification of Amazonian stingless bees using polymerase chain reaction single-strand conformation polymorphism

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

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