“…Rainbow trout (Oncorhynchus mykiss) juveniles (10.6±0.2 cm and 12.2±0.5 g of total length and mass, respectively) were acquired at an aquaculture facility, Posto Aquícola do Torno, in Marão, north of Portugal. Rainbow trout is a freshwater fish species used for human consumption, easy to maintain in laboratory, highly abundant and it is a standard species in toxicology assays (Talbot, 2014). Fish were collected using hand nets and then transported in plastic bags with continuous air supply on cold water until the arrival at the laboratory.…”