FISH Variants

Guimarães, Nuno; Azevedo, Nuno F.; Almeida, Carina

doi:10.1007/978-1-0716-1115-9_2

Cited by 7 publications

(4 citation statements)

References 82 publications

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“…FISH can be performed on a variety of different sample types from living samples, fresh tissue, frozen tissue, and formalin-fixed tissue. Tests can be run individually or multiplexed to combine probes against multiple samples, simultaneously [ 18 , 19 , 21 , 34 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 ].…”

Section: Discussionmentioning

confidence: 99%

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Rolph,

Bolfa,

Cavanaugh

et al. 2024

Veterinary Sciences

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FISH techniques have been applied for the visualization and identification of intracellular bacteria in companion animal species. Most frequently, these techniques have focused on the identification of adhesive-invasive Escherichia coli in gastrointestinal disease, although various other organisms have been identified in inflammatory or neoplastic gastrointestinal disease. Previous studies have investigated a potential role of Helicobacter spp. in inflammatory gastrointestinal and hepatic conditions. Other studies evaluating the role of infectious organisms in hepatopathies have received some attention with mixed results. FISH techniques using both eubacterial and species-specific probes have been applied in inflammatory cardiovascular, urinary, and cutaneous diseases to screen for intracellular bacteria. This review summarizes the results of these studies.

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Section: Discussionmentioning

confidence: 99%

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Rolph,

Bolfa,

Cavanaugh

et al. 2024

Veterinary Sciences

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“…Accurate detection and analysis are facilitated through the utilization of fluorophores that exhibit closely overlapping excitation and emission spectra. The combinatorial labeling approach entails the utilization of a preset repertoire of several fluorophores to selectively label a particular bacterium [ 98 , 99 , 100 , 101 ]. By implementing this combinatorial labeling technique, the range of distinguishable microbial taxa within a specific visual area is substantially increased [ 99 , 100 ].…”

Section: Evolution: Microorganism To Microbiomementioning

confidence: 99%

Microbial Symphony: Navigating the Intricacies of the Human Oral Microbiome and Its Impact on Health

Bhandary,

Venugopalan,

Ramesh

et al. 2024

Microorganisms

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This comprehensive review delves into the forefront of research on the human oral microbiome, exploring recent advancements that span microbial colonization, state-of-the-art detection methodologies, and the complex interplay involved in disease progression. Through an exhaustive analysis of the contemporary literature, we illuminate the dynamic orchestration of microbial communities within the oral cavity, underscoring their pivotal role in health and disease. Cutting-edge detection techniques, including metagenomics and high-throughput sequencing, are discussed regarding their transformative impact on understanding the intricacies of oral microbial ecosystems. As we stand on the cusp of a new decade, this review anticipates a paradigm shift in the field, emphasizing the potential for rapid identification and targeted management of detrimental oral microorganisms. Insights gained from this exploration not only contribute to our fundamental understanding of the oral microbiome but also hold promise for the development of innovative therapeutic strategies to maintain oral health. This article aims to serve as a valuable resource for researchers, clinicians, and public health professionals engaged in unraveling the mysteries of the microbial symphony within the human oral cavity.

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“…FISH is one of the most routinely used cytochemical techniques for genetic detection, identification, and localization by a fluorescently labeled nucleic acid probe. It is more specifically used in hybridizing processes with nucleic acid sequences of interest [16]. The vitality of this technology is continuously proved by the evolution of probe design, signal amplification, and multiplexing, broadening the application of experimental research and clinical diagnoses in this field of study [17].…”

Section: Research Progress Of Fishmentioning

confidence: 99%

“…The procedure and principles of these three currently used FISH techniques in oral microbiology will be elaborated throughout this study. The most common procedure followed by researchers who apply FISH involves the following four steps: (1) fixation for dehydration to inhibit the action of enzymes without cell and nucleic acid structures destruction (some special cells need extra permeabilization treatment that degrade the cell walls to increase membrane permeability); (2) hybridization between target nucleic acid sequences and specific complementary probes labeled with fluorescent dyes or reporter molecules to be detected by fluorescent antibodies; (3) washing to remove the unbound or loosely bound (usually mismatched) probes; (4) detection and visualization of the probe-bounded cells by typically fluorescence microscopy and some advanced microscopy systems, then access to information for species detection or microbiota analysis [16,36,87]. Hybridization is one of the most decisive steps, strongly influenced by probe properties on specificity and sensitivity [87].…”

Section: Procedures and Principles Of Fish And Its Variants Used For ...mentioning

confidence: 99%

Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection

Wang

Zhang

et al. 2022

Pathogens

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Varieties of microorganisms reside in the oral cavity contributing to the occurrence and development of microbes associated with oral diseases; however, the distribution and in situ abundance in the biofilm are still unclear. In order to promote the understanding of the ecosystem of oral microbiota and the diagnosis of oral diseases, it is necessary to monitor and compare the oral microorganisms from different niches of the oral cavity in situ. The fluorescence in situ hybridization (FISH) has proven to be a powerful tool for representing the status of oral microorganisms in the oral cavity. FISH is one of the most routinely used cytochemical techniques for genetic detection, identification, and localization by a fluorescently labeled nucleic acid probe, which can hybridize with targeted nucleic acid sequences. It has the advantages of rapidity, safety, high sensitivity, and specificity. FISH allows the identification and quantification of different oral microorganisms simultaneously. It can also visualize microorganisms by combining with other molecular biology technologies to represent the distribution of each microbial community in the oral biofilm. In this review, we summarized and discussed the development of FISH technology and the application of FISH in oral disease diagnosis and oral ecosystem research, highlighted its advantages in oral microbiology, listed the existing problems, and provided suggestions for future development..

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FISH Variants

Cited by 7 publications

References 82 publications

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Microbial Symphony: Navigating the Intricacies of the Human Oral Microbiome and Its Impact on Health

Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection

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