Fission Yeast HMT1 Lowers Seed Cadmium through Phytochelatin-Dependent Vacuolar Sequestration in Arabidopsis

Huang, Jing; Zhang, Yu; Peng, Jia-Shi; Chen, Zhong; Yi, Hong-Ying; Ow, David W.; Gong, Ji-Ming

doi:10.1104/pp.111.192872

Cited by 80 publications

(36 citation statements)

References 48 publications

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“…While protein phosphorylation has been shown to prompt PCS synthesis during GSH production , there are no known reports of protein phosphatases involved in PC regulation. Huang et al (2012) reported that Cd tolerance and accumulation failed when GSH and PC synthesis was blocked by L-buthionine sulfoximine, and also when PC synthesis was blocked in the PCS-deficient cad1-3 mutant.…”

Section: Phytochelatinsmentioning

confidence: 98%

Cadmium Bioavailability, Uptake, Toxicity and Detoxification in Soil-Plant System

Shahid

Dumat

Khalid

et al. 2016

Reviews of Environmental Contamination and Toxicology

231

175

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This review summarizes the findings of the most recent studies, published from 2000 to 2016, which focus on the biogeochemical behavior of Cd in soil-plant systems and its impact on the ecosystem. For animals and people not subjected to a Cd-contaminated environment, consumption of Cd contaminated food (vegetables, cereals, pulses and legumes) is the main source of Cd exposure. As Cd does not have any known biological function, and can further cause serious deleterious effects both in plants and mammalian consumers, cycling of Cd within the soil-plant system is of high global relevance.The main source of Cd in soil is that which originates as emissions from various industrial processes. Within soil, Cd occurs in various chemical forms which differ greatly with respect to their lability and phytoavailability. Cadmium has a high phytoaccumulation index because of its low adsorption coefficient and high soil-plant mobility and thereby may enter the food chain. Plant uptake of Cd is believed to occur mainly via roots by specific and non-specific transporters of essential nutrients, as no Cd-specific transporter has yet been identified. Within plants, Cd causes phytotoxicity by decreasing nutrient uptake, inhibiting photosynthesis, plant growth and respiration, inducing lipid peroxidation and altering the antioxidant system and functioning of membranes. Plants tackle Cd toxicity via different defense strategies such as decreased Cd uptake or sequestration into vacuoles. In addition, various antioxidants combat Cd-induced overproduction of ROS. Other mechanisms involve the induction of phytochelatins, glutathione and salicylic acid.

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Section: Phytochelatinsmentioning

confidence: 98%

Cadmium Bioavailability, Uptake, Toxicity and Detoxification in Soil-Plant System

Shahid

Dumat

Khalid

et al. 2016

Reviews of Environmental Contamination and Toxicology

231

175

View full text Add to dashboard Cite

show abstract

“…In plants, PCs can confer tolerance to heavy metal, as indicated by numerous recent studies, including Cd tolerance in B. juncea (Mohamed et al 2012), Populus nigra (Iori et al 2012), Oenothera odorata (Son et al 2012), T. aestivum (Hentz et al 2012), and Linum usitatissimum (Najmanova et al 2012); As tolerance in Nicotiana tabacum (Shukla et al 2012) and Arabidopsis thaliana (Huang et al 2012); Zn and Cu tolerance in Arabidopsis thaliana (Huang et al 2012); and Mn tolerance in Vitis vinifera (Yao et al 2012). Glutathione conjugates metals by forming PCs, which can be translocated.…”

Section: Toxic Metals/metalloidsmentioning

confidence: 99%

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Hasanuzzaman

Nahar

Anee

et al. 2017

Physiol Mol Biol Plants

618

284

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Glutathione (GSH; c-glutamyl-cysteinyl-glycine) is a small intracellular thiol molecule which is considered as a strong non-enzymatic antioxidant. Glutathione regulates multiple metabolic functions; for example, it protects membranes by maintaining the reduced state of both a-tocopherol and zeaxanthin, it prevents the oxidative denaturation of proteins under stress conditions by protecting their thiol groups, and it serves as a substrate for both glutathione peroxidase and glutathione S-transferase. By acting as a precursor of phytochelatins, GSH helps in the chelating of toxic metals/metalloids which are then transported and sequestered in the vacuole. The glyoxalase pathway (consisting of glyoxalase I and glyoxalase II enzymes) for detoxification of methylglyoxal, a cytotoxic molecule, also requires GSH in the first reaction step. For these reasons, much attention has recently been directed to elucidation of the role of this molecule in conferring tolerance to abiotic stress. Recently, this molecule has drawn much attention because of its interaction with other signaling molecules and phytohormones. In this review, we have discussed the recent progress in GSH biosynthesis, metabolism and its role in abiotic stress tolerance.

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“…Similar to the above assays, root tissues (1.0 g) were collected from B. napus at seedling and flowering stages and from Arabidopsis (0.5 g) seedlings and used to isolate intact protoplasts and vacuoles as described by Robert et al (2007), with minor modifications as outlined in Huang et al (2012) and Han et al (2015b). The purified protoplasts were divided into two equal aliquots, with one of them used for isolation of vacuoles.…”

Section: Acc Treatmentmentioning

confidence: 99%

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

et al. 2016

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Enhancing nitrogen use efficiency (NUE) in crop plants is an important breeding target to reduce excessive use of chemical fertilizers, with substantial benefits to farmers and the environment. In Arabidopsis (Arabidopsis thaliana), allocation of more NO 3 2 to shoots was associated with higher NUE; however, the commonality of this process across plant species have not been sufficiently studied. Two Brassica napus genotypes were identified with high and low NUE. We found that activities of V-ATPase and V-PPase, the two tonoplast proton-pumps, were significantly lower in roots of the high-NUE genotype (Xiangyou15) ] S:R ratios were significantly higher in Xiangyou15. BnNRT1.5 expression was higher in roots of Xiangyou15 compared with 814, while BnNRT1.8 expression was lower. In both B. napus treated with proton pump inhibitors or Arabidopsis mutants impaired in proton pump activity, vacuolar sequestration capacity (VSC) of NO 3 2 in roots substantially decreased. Expression of NRT1.5 was up-regulated, but NRT1.8 was down-regulated, driving greater NO 3 2 long-distance transport from roots to shoots. NUE in Arabidopsis mutants impaired in proton pumps was also significantly higher than in the wild type col-0. Taken together, these data suggest that decrease in VSC of NO 3 2 in roots will enhance transport to shoot and essentially contribute to higher NUE by promoting NO 3 2 allocation to aerial parts, likely through coordinated regulation of NRT1.5 and NRT1.8.

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Fission Yeast HMT1 Lowers Seed Cadmium through Phytochelatin-Dependent Vacuolar Sequestration in Arabidopsis

Cited by 80 publications

References 48 publications

Cadmium Bioavailability, Uptake, Toxicity and Detoxification in Soil-Plant System

Cadmium Bioavailability, Uptake, Toxicity and Detoxification in Soil-Plant System

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

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