Five different phenotypes of endothelial cell cultures from the bovine corpus luteum: Present outcome and role of potential dendritic cells in luteolysis

Spanel‐Borowski, Katharina

doi:10.1016/j.mce.2011.02.021

Cited by 8 publications

(3 citation statements)

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“…Cytokeratin-positive cells were more polygonal and grew in a cobblestone pattern that is commonly associated with endothelial cells, as observed in this study. In addition, the different phenotypes of endothelial cells have been described according to the cytokeratin pattern, and cytokeratin-positive cells in the microvasculature have been thought to be danger-sensing cells [44]. In fibroblasts, cytokeratin expression is reported to be low, but some keratins have been shown to be present in fibroblasts in vitro [45].…”

Section: Discussionmentioning

confidence: 99%

Lytic cycle of Besnoitia besnoiti tachyzoites displays similar features in primary bovine endothelial cells and fibroblasts

et al. 2019

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BackgroundBovine besnoitiosis, caused by the cyst-forming apicomplexan parasite Besnoitia besnoiti, is a chronic and debilitating cattle disease that continues to spread in Europe in the absence of control tools. In this scenario, in vitro culture systems are valuable tools to carry out drug screenings and to unravel host-parasite interactions. However, studies performed in bovine target cells are scarce.MethodsThe objective of the present study was to obtain primary bovine aortic endothelial cells (BAECs) and fibroblast cell cultures, target cells during the acute and the chronic stage of the disease, respectively, from healthy bovine donors. Afterwards, expression of surface (CD31, CD34 and CD44) and intracellular markers (vimentin and cytokeratin) was studied to characterize cell populations by flow cytometry. Next, the lytic cycle of B. besnoiti tachyzoites was studied in both target cells. Invasion rates (IRs) were determined by immunofluorescence at several time points post-infection, and proliferation kinetics were studied by quantitative PCR (qPCR). Finally, the influence of bovine viral diarrhea virus (BVDV) co-infection on the host cell machinery, and consequently on B. besnoiti invasion and proliferation, was investigated in BAECs.ResultsMorphology and cytometry results confirmed the endothelial and fibroblast origins. CD31 was the surface marker that best discriminated between BAECs and fibroblasts, since fibroblasts lacked CD31 labelling. Expression of CD34 was weak in low-passage BAECs and absent in high-passage BAECs and fibroblasts. Positive labelling for CD44, vimentin and cytokeratin was observed in both BAECs and fibroblasts. Regarding the lytic cycle of the parasite, although low invasion rates (approximately 3–4%) were found in both cell culture systems, more invasion was observed in BAECs at 24 and 72 hpi. The proliferation kinetics did not differ between BAECs and fibroblasts. BVDV infection favoured early Besnoitia invasion but there was no difference in tachyzoite yields observed in BVDV-BAECs compared to BAECs.ConclusionsWe have generated and characterized two novel standardized in vitro models for Besnoitia besnoiti infection based on bovine primary target BAECs and fibroblasts, and have shown the relevance of BVDV coinfections, which should be considered in further studies with other cattle pathogens.

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Section: Discussionmentioning

confidence: 99%

Lytic cycle of Besnoitia besnoiti tachyzoites displays similar features in primary bovine endothelial cells and fibroblasts

et al. 2019

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“…As figure 2, the corpus luteum is a heterogeneous tissue comprising large luteal cells, small luteal cells, endothelial cells, pericytes, fibroblasts, smooth muscle cells, and other cell types [56]. Those heterogeneous cell populations of corpus luteum can be categorized into two main representatives of cell types: non-steroidogenic cells, such as microvascular cells, connective tissue cells, and immune cells, and steroidogenic cells, such as large luteal cells and small luteal cells [57]. LGC, luteal granulosa cell; LTC, luteal theca cell.…”

Section: Luteal Cell Types Of the Corpus Luteummentioning

confidence: 99%

The Functions of Reproductive Hormones and Luteal Cell Types of Ovarian Corpus Luteum in Cows

Billhaq¹,

Lee²

2023

Preprint

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The corpus luteum is a temporary reproductive endocrine structure established from a ruptured follicle wall after ovulation. In the ovarian cycle of ruminant species, the corpus luteum organ undergoes a repeated pattern of specific cellular proliferation, differentiation, and transformation. The corpus luteum encounters several physiological events, including growth, function, and regression throughout its life span. Also, the corpus luteum comprises granulosa and theca cells and endothelial cells, such as luteal steroidogenic cells and luteal endothelial cells. These cells play an important role in the physiology of the corpus luteum and the maintenance and degeneration of the corpus luteum. Furthermore, the role of reproductive hormones in the ovaries is important. Representative hormones include estrogen, progesterone, prostaglandin F2α, and oxytocin. Understanding their functions is important in studying the physiological phenomena and various mechanisms of the corpus luteum in the ovary. Therefore, the following review will discuss the role of reproductive hormones and luteal cell types in the microenvironment of the corpus luteum in the bovine ovary.

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“…Pate 1995; Penny 2000), neutrophils (Shirasuna et al. 2012) and dendritic cells (Spanel‐Borowski 2011). Some recent work has focused on the chemokine and adhesion molecules that may be responsible for trafficking of immune cells into the CL, including prokineticin (Kisliouk et al.…”

Section: Heterogeneous Cells Involved In Luteolysismentioning

confidence: 99%

Life or Death Decisions in the Corpus Luteum

Pate

Johnson-Larson

Ottobre

2012

Reprod Domestic Animals

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Contents The corpus luteum (CL) is an ephemeral endocrine organ. During its lifespan, it undergoes a period of extremely rapid growth that involves hypertrophy, proliferation and differentiation of the steroidogenic cells, as well as extensive angiogenesis. The growth phase is followed by a period in which remodelling of the tissue ceases, but it engages in unparalleled production of steroids, resulting in extraordinarily high metabolic activity within the tissue. It is during this stage that a critical juncture occurs. In the non‐fertile cycle, uterine release of prostaglandin (PG)F2α initiates a cascade of events that result in rapid loss of steroidogenesis and destruction of the luteal tissue. Alternatively, if a viable embryo is present, signals are produced that result in rescue of the CL. This review article summarizes the major concepts related to the fate of the CL, with particular focus on recent insights into the mechanisms associated with the ability of PGF2α to bring about complete luteolysis. It has become clear that the achievement of luteolysis depends on repeated exposure to PGF2α and involves coordinated actions of heterogeneous cell types within the CL. Together, these components of the process bring about not only the loss in progesterone production, but also the rapid demise of the structure itself.

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Five different phenotypes of endothelial cell cultures from the bovine corpus luteum: Present outcome and role of potential dendritic cells in luteolysis

Abstract: Please cite this article as: Spanel-Borowski, K., Five different phenotypes of endothelial cell cultures from the bovine corpus luteum: present outcome and role of potential dendritic cells in luteolysis, Molecular and Cellular Endocrinology (2010),

Cited by 8 publications

References 89 publications

Lytic cycle of Besnoitia besnoiti tachyzoites displays similar features in primary bovine endothelial cells and fibroblasts

Lytic cycle of Besnoitia besnoiti tachyzoites displays similar features in primary bovine endothelial cells and fibroblasts

The Functions of Reproductive Hormones and Luteal Cell Types of Ovarian Corpus Luteum in Cows

Life or Death Decisions in the Corpus Luteum

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