Five-Hour Detection of Intestinal Colonization with Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae Using the β-Lacta Phenotypic Test: the BLESSED Study

Abstract

To both improve the efficiency of contact isolation among ESBL-PE carriers and avoid the unnecessary isolation of noncolonized patients, we should reduce the turnaround time of ESBL screening in laboratories and improve the sensitivity of diagnostic methods. The development of rapid and low-cost methods that satisfy these two goals is a promising approach.

Detection of blaCTX-M and blaDHA genes in stool samples of healthy people: comparison of culture- and shotgun metagenomic-based approaches

“…However, considering that the concentration of some ESC-R- Ent in the gut may be lower than the technical LOD (~10 1 CFU/100 mg) and the commensal bacteria (e.g., Bacteroides spp. ), the native culture approach may not provide sufficient resolution (e.g., resulting in visible bacterial growth in culture plates) to detect these pathogens, unless a pre-enrichment step is performed ( Naas et al, 2011 ; Girlich et al, 2014 ; Rondinaud et al, 2020 ; Sadek et al, 2020 ; Gallah et al, 2023 ).…”

“…In these culture-based approaches, native stool or perianal/perirectal swab samples are directly plated on selective chromogenic media ( Blane et al, 2016 ; Girlich et al, 2019 ). A broth pre-enrichment step (with or without antibiotics) may also be added before plating to increase the detection rate of ESC-R- Ent ( Blane et al, 2016 ; Jazmati et al, 2016 , 2017 ; Rondinaud et al, 2020 ; Gallah et al, 2023 ). Though, this approach is seldom implemented due to its increased hands-on time and turnaround time (TAT).…”