Five New Methods for the Spectrophotometic Determination of Alkylating Agents Including Some Extremely Sensitive Autocatalytic Methods. Application to Air Pollution.

Chemical composition of tobacco and tobacco smoke

“…Although the presence of alkylating activity in cigarette smoke has been known for several years (424,487), details of this property have been studied only recently (542). Alkylations are primarily nucleophilic reactions and a colorimetric method employing the nucleophile 4-(4'-nitrobenzyl)pyridine has been used to determine the level and distribution of activity in cigarette smoke condensate.…”

Analysis of insect chemosterilants. Action of phosphate buffers on aziridine

“…When chemicals, especially alkylating agents, are to be used to produce sterile insects, it is essential to measure the period of persistence of these substances and their decomposition products. Several methods of analysis for chemosterilants such as aziridine-containing compounds (1-5) and alkylating agents such as alkyl-halides and sulfonates (6,7) have been reported and have been widely used in degradation and metabolic studies. These kinetic studies were performed in conjunction with those used in the development of an auto-mated analytical procedure for insect chemosterilants (S, 9).…”

“…Soc., 79, 6157 (1957). An automated punched-tape system for gradient elution chromatography (/), developed in our laboratories, has been used successfully for the analysis of adrenocortical steroids in biological mixtures (2)(3)(4)(5)(6). Application of the method to analysis of the residues from incubation of tissue with radioactive steroids and precursors revealed a need for further refinement in the apparatus.…”

4-(4-nitrobenzyl)pyridine tests for alkylating agents following chemical oxidative activation

“…Several alkylating agents were assayed using NBP as an analytical agent for estimation of their alkylating activities; it has been used to assay herbicides such as dimethyl tetrachloroterephthalate (Schuldt et al 1961), organophosphate pesticides (Getz and Watts 1964;Ragab 1967); nitrogen mustards (Friedman and Boger 1961); haloalkanes and alkenes (Sawicki et al 1963); azirdines (Bardos et al 1965) nitrosourea compounds (Yano and Isobe 1979);; epoxides (Hemminki et al 1980;Ehrenberg and Hussain 1981;Voogd et al 1981;Hemminki and Hesso 1984).…”