Fixed-time AI pregnancy rate following insemination with frozen-thawed or fresh-extended semen in progesterone supplemented CO-Synch protocol in beef cows

Bucher, Andreas; Kasimanickam, R.; Hall, J. B.; DeJarnette, J.M.; Whittier, W. Dee; Kähn, W.; Xu, Z.Z.

doi:10.1016/j.theriogenology.2008.12.009

Cited by 36 publications

(30 citation statements)

References 15 publications

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“…The major benefits linked to the utilization of cool-stored semen are a greater viability than frozen semen, the chance of consuming little doses for insemination, reduced expenses of storage, and the ease for utilizing in AI [3]. Moreover, chilled semen has more viability in the reproductive tract of female and guaranteeing greater rates of fertilization [4]. …”

Section: Introductionmentioning

confidence: 99%

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

et al. 2017

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Aim:The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters.Materials and Methods:A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test.Results:The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups.Conclusion:In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment.

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Section: Introductionmentioning

confidence: 99%

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

et al. 2017

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“…Greater understanding of follicular dynamics (for reviews, see Fortune et al 2001;Ginther et al 2001) has increased the possibility of controlling follicular development through hormonal treatment protocols, which allow for fixed-timed AI (FTAI) in both Bos taurus (Pursley et al 1995;Martinez et al 2002;Perry et al 2002;Bucher et al 2009;Larson et al 2009) and Bos indicus (Barros et al 2000;Fernandes et al 2001;Ayres et al 2008;Carvalho et al 2008;Meneghetti et al 2009;Pinheiro et al 2009;Sa Filho et al 2009) cattle. Similarly, follicular development and ovulation have been manipulated to improve the management of superovulation and embryo transfer (Barros and Nogueira 2001;Mapletoft et al 2002;Mapletoft and Hasler 2005;Baruselli et al 2006;Nogueira et al 2007b;Bó et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of superstimulatory treatments on the expression of genes related to ovulatory capacity, oocyte competence and embryo development in cattle

Barros

Satrapa

Castilho

et al. 2013

Reprod. Fertil. Dev.

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Multiple ovulation (superovulation) and embryo transfer has been used extensively in cattle. In the past decade, superstimulatory treatment protocols that synchronise follicle growth and ovulation, allowing for improved donor management and fixed-time AI (FTAI), have been developed for zebu (Bos indicus) and European (Bos taurus) breeds of cattle. There is evidence that additional stimulus with LH (through the administration of exogenous LH or equine chorionic gonadotrophin (eCG)) on the last day of the superstimulatory treatment protocol, called the 'P-36 protocol' for FTAI, can increase embryo yield compared with conventional protocols that are based on the detection of oestrus. However, inconsistent results with the use of hormones that stimulate LH receptors (LHR) have prompted further studies on the roles of LH and its receptors in ovulatory capacity (acquisition of LHR in granulosa cells), oocyte competence and embryo quality in superstimulated cattle. Recent experiments have shown that superstimulation with FSH increases mRNA expression of LHR and angiotensin AT(2) receptors in granulosa cells of follicles >8 mm in diameter. In addition, FSH decreases mRNA expression of growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) in oocytes, but increases the expression of both in cumulus cells, without diminishing the capacity of cumulus-oocyte complexes to generate blastocysts. Although these results indicate that superstimulation with FSH is not detrimental to oocyte competence, supplementary studies are warranted to investigate the effects of superstimulation on embryo quality and viability. In addition, experiments comparing the cellular and/or molecular effects of adding eCG to the P-36 treatment protocol are being conducted to elucidate the effects of superstimulatory protocols on the yield of viable embryos.

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“…Synchronization rate was similiar (85-91%) to some others (3,9,17,26). Synchronization rate was ranged 43 to 60% of beef and dairy heifers without presynchronization (31).…”

Section: Discussionmentioning

confidence: 62%

Conception rate following timed artificial insemination protocols in dairy heifers synchronised by PGF2α and GnRH

;MECİTOĞLU¹

2011

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study aimed to develop a timed artificial insemination (TAI) protocol using PGF 2α and GnRH with acceptable pregnancy rate in dairy heifers. Dairy heifers (n=290), aged between 13 and 26 months (mean 16.1 months), were used in this study. Heifers were synchronized with two PGF 2α administrations by 14 d intervals. After the second PGF 2α , heifers were randomly assigned into two groups. In Group 1 (n=155), GnRH was given at 56h following 2 nd PGF 2α and TAI was performed at 16-18h after the GnRH. In Group 2 (n=135), GnRH was administered at 72h after 2 nd PGF 2α , at the time of TAI. Ultrasonography was performed at both PGF 2α administrations to determine cyclicity, at the time of TAI to measure ovulatory follicle size, 7 d after TAI to determine ovulation, 31 and 62 d post-AI to determine pregnancy. Ovulatory follicle size at the time of TAI was not significantly different between groups (12.8±1.6 mm and 13.2±1.8 mm in group 1 and 2, respectively). Synchronization rate was similar in group 1 (85.2%;132/155) and in group 2 (91.1%;123/135). Conception rate (CR) at 31 d was not different in group 1 (59.8% ;79/132) and in group 2 (55.3%;68/123). Embryonic loss (7.6%; 6/79 and 7.4%; 5/68 in group 1 and 2 respectively) was not different. Gender ratio of the calves was found different between groups. Proportion of the female calves born were higher (P=0.03) in group 1 (63.3%; 38/60) than in group 2 (42.0%; 21/50). In conclusion, both protocols can be used in order to eliminate estrous detection in large dairy herds. Although GnRH administration at the time of TAI was found to be useful to reduce handling of heifers, GnRH can be applied 16-18 h before AI to achive higher female calves ratio in large dairy farms. Keywords: Dairy heifers, GnRH, PGF 2α , timed artificial insemination, synchronization. PGF 2α ve GnRH Kullanılarak Senkronize Edilen Düvelerde Zaman Ayarlı SuniTohumlama Sonrasmda Elde Edilen Gebelik OranlarıÖzet: Bu çalışmanın amacı GnRH ve PGF 2α kullanılarak senkronize edilen düvelerde, östrüs kontrolleri yapılmadan uygulanan zaman ayarlı suni tohumlama sonrasında istenilen düzeyde gebelik oranı elde etmekti. Çalışmada yaşları 13 ile 24 ay arasında değişen (ortalama 16.1) düveler (n=290) kullanıldı. Düveler 14 gün ara ile uygulanan PGF 2α ile senkronize edildi ve ikinci PGF 2α uygulaması sonrası rastgele iki gruba ayrıldı. Grup l'e (n=155) ikinci PGF 2α uygulamasını takiben 56. saat GnRH ve GnRH uygulamasını taiben 16-18. saat zaman ayarlı suni tohumlama yapıldı. Grup 2'ye ise (n=135) ikinci PGF 2α uygulamasını takiben 72. saatte suni tohumlama GnRH ile birlikte uygulandı. Ovaryumların siklik olup olmadığını belirlemek amacıyla her iki PGF 2α uygulaması sırasında, ovaryumlarda folliküllerin çapını belirmek amacıyla suni tohumlama ile eş zamanlı olarak, ovulasyon oranını belirlemek amacıyla suni tohumlamayı takiben 7. gün, gebelik oranını belirlemek amacıyla suni tohumlamayı takiben 31 ve 62. günlerde ultrasonografi uygulaması yapıldı. Suni tohumlama ile eş zamanlı olarak belirlenen follikül ça...

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Fixed-time AI pregnancy rate following insemination with frozen-thawed or fresh-extended semen in progesterone supplemented CO-Synch protocol in beef cows

Cited by 36 publications

References 15 publications

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

Effect of superstimulatory treatments on the expression of genes related to ovulatory capacity, oocyte competence and embryo development in cattle

Conception rate following timed artificial insemination protocols in dairy heifers synchronised by PGF2α and GnRH

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