In a constant-volume chamber, the atomization characteristics of low-viscosity fuel sprays were studied under the effects of mass concentration and injection pressure. Microscopic spray parameters were measured via laser diffraction downstream of the nozzle at various axial and radial positions. The results showed that an increase in the mass concentration suppressed the atomization effect in a linear manner. The increase in injection pressure promoted droplet breakup; however, the trend gradually weakened and became more noticeable at high concentrations. Compared to the concentration, the influence of the injection pressure on the atomization characteristics was dominant. Although low concentration and high injection pressure promoted droplet breakup, they also increased the probability of droplet collision, resulting in droplet aggregation. This was more evident in low-viscosity fuels. The droplet size increased in the axial direction owing to the aggregation. However, the diameter decreased in the radial direction owing to the outward deflection of small droplets caused by air turbulence and entrainment. In addition, the high-velocity airflow significantly promoted droplet breakup near the nozzle and spray axis regions and suppressed the aggregation effect. Nevertheless, lower-viscosity fuels maintained smaller droplet sizes and better atomization throughout the spraying process, which was easier to achieve than that in higher-viscosity fuels. Overall, low concentration, high injection pressure and low viscosity of the fuel have beneficial effects on the droplet breakup. This is important to enhance the atomization effect of the fuel.