Flat panel airlift photobioreactors for cultivation of vegetative cells of microalga Haematococcus pluvialis

Issarapayup, Kerati; Powtongsook, Sorawit; Pavasant, Prasert

doi:10.1016/j.jbiotec.2009.04.014

Cited by 64 publications

(26 citation statements)

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“…and doubling time (6.9 days) rates. This is due to adequate level of circulation in the system which may provide better growth rates (Issarapayup et al, 2009). Further, the 13-L volume warranted the better exposure of cells to light and provided more significant photosynthesis that with the culture of the 250-L volume.…”

Section: Discussionmentioning

confidence: 99%

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

2013

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Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 10 5 cells.mL -1 on the 12 th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions. Keywords: biological aspects, growth, nutritional content, microalgae. Efeito de alguns parâmetros no cultivo em larga escala de Haematococcus pluvialis Flotow ResumoForam avaliadas a taxa de crescimento e condições do meio de cultura em volumes de 13-L e 250-L em sistema estático, durante o período de 13 dias para a microalga Haematococcus pluvialis. Foi determinada qualitativamente a contagem de células, densidade, peso seco, teor de cinzas, clorofila-a, teor de carbono orgânico total e avaliação de nutrientes e fatores abióticos do meio de cultura. O crescimento foi mais elevado em volume de 13-L com 1,33 x 10 5 células.mL -1 no décimo segundo dia, do que em volume de 250-L. Em relação ao meio de cultivo e aspectos biológicos de H. pluvialis, foram observadas diferenças significativas (p < 0,05) entre os dois volumes com exceção do peso seco, cinzas, nitrito e amônia. Para cultivo em larga escale de H. pluvialis é recomendado nestas condições laboratoriais, o volume de 13-L devido ao maior tempo das células em estado vegetativo, maior densidade celular e elevados teores de lipídios e clorofila-a. A luz interferiu diretamente nas condições biológicas de H. pluvialis. Palavras-chave: aspectos biológicos, crescimento, conteúdo nutricional, microalga.

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Section: Discussionmentioning

confidence: 99%

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

2013

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“…La velocidad específica máxima de crecimiento (µ), obtenida en la fase exponencial, se calculó de acuerdo con la siguiente ecuación (Issarapayup et al 2009…”

Section: Materiales Y Métodosunclassified

“…Por lo anterior, diversos autores han evaluado diferentes diseños de fotobiorreactores para asegurar el uso eficiente de la luz a la intemperie en cultivo semicontinuo (Innok et al 2009). Por ejemplo, los diseños para la producción de Tetraselmis subcordiformis en un fotobiorreactor de puente aéreo rectangular de 10.5 L (Zheng et al 2011) y Haematococcus pluvialis en un fotobiorreactor de puente aéreo de cara plana de 17 L (Issarapayup et al 2009). Asimismo, Travieso et al (2001) utilizaron un sistema semicontinuo con diferentes tasas de dilución en un fotobiorreactor tubular helicoidal de 20 L para la producción de Spirulina platensis.…”

Section: Introductionunclassified

Producción a La Intemperie De Un Consorcio Microbiano Fotosintético Fijador De Nitrógeno en Cultivo Por Lote Y Semicontinuo en Un Fotobiorreactor De Puente Aéreo De Cara Plana

Melchor

Cristiani‐Urbina

Cerrato

et al. 2017

Rev. Int. Contam. Ambie.

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Palabras clave: cianobacteria, autotrofía, biofertilizante RESUMENLas cianobacterias son organismos fotótrofos oxigénicos que tienen la capacidad de formar consorcios. Una estrategia que ha sido utilizada para mejorar la productividad de cianobacterias y microalgas en fotobiorreactores es producirlas en cultivo semicontinuo. En nuestro grupo de trabajo hemos estudiado un consorcio microbiano (CM) fotosintético con capacidad de fijar nitrógeno, constituido principalmente por cianobacterias, el cual tiene uso potencial como biofertilizante. El objetivo de este estudio fue evaluar la capacidad de producción de biomasa del CM en dos fotobiorreactores (FBR) de puente aéreo de cara plana (50 y 150 L) a la intemperie, en cultivo por lote en ambos FBR y en cultivo semicontinuo en el FBR de mayor volumen. La máxima concentración de biomasa en cultivo por lote se obtuvo en el FBR de 150 L, con un valor de 600 mg/L, en comparación a la biomasa obtenida en el FBR de 50 L, la cual fue igual a 385 mg/L. La productividad de biomasa en los FBR de 50 y 150 L fue de 0.77 y 1.19 mg/L/h, respectivamente. El cultivo del CM en semicontinuo se realizó por tres ciclos (7, 7 y 5 d cada uno, respectivamente). Se obtuvo un aumento de la productividad de biomasa tras cada ciclo, con valores de 1.86, 1.94 y 2.94 mg/L/h. Asimismo, los valores máximos de actividad de nitrogenasa se obtuvieron al final de cada ciclo, los cuales fueron de 4963, 5366 y 5689 nmol eteno/g peso seco/h, respectivamente. Estos valores fueron superiores a los obtenidos en cultivo por lote. En conclusión, es factible producir el CM en los FBR en cultivo por lote y semicontinuo a la intemperie, sin afectar su capacidad de fijación de nitrógeno.Key words: cyanobacteria, autotrophy, biofertilizer 74 ABSTRACTCyanobacteria are oxygenic phototrophic organisms that have the capacity to form consortia. One strategy that has been used to improve the productivity of cyanobacteria and algae in photobioreactors is to produce them in semicontinuous cultures. In our research group, a photosynthetic microbial consortium (MC) with nitrogen fixing capacity was studied. It consists mainly of cyanobacteria and could potentially be used as a biofertilizer. The aim of this study was to evaluate the capability to produce biomass of MC in two flat-panel airlift photobioreactors (PBR) (50 y 150 L) in batch culture, and in semicontinuous culture in a PBR with 150 L capacity, outdoors. The maximum biomass concentration in batch culture was obtained in the PBR of 150 L, with a value of 600 mg/L, in comparison with the biomass concentration obtained in the PBR of 50 L, which was equal to 385 mg/L. The biomass productivity at the end of the batch culture in both PBR (50 and 150 L) was 0.77 and 1.19 mg/L/h, respectively. The semicontinuous culture of CM was operated during three cycles (7, 7 and 5 d, respectively), attaining an increase in the biomass productivity following each cycle, with values of 1.86, 1.94 and 2.94 mg/L/h, respectively. Similarly, the maximum values of the nitrogenase activit...

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“…40 %, the cost of culture medium is ca. 6 % [ 116 ]; biomass harvesting contributes up to 30 % to the total cost of product. The rest is comprised by the cost of biomass dehydration [ 92 ].…”

Section: Economic Aspects Of Carotenoid Production With Microalgaementioning

confidence: 99%

Production of Carotenoids Using Microalgae Cultivated in Photobioreactors

Solovchenko

Chekanov

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Carotenoids comprise a diverse group of natural biomolecules with a plethora of benefi cial effects. These compounds include potent bioantioxidants, provitamins, and safe colourants that are in high demand by pharmaceutical, cosmetic and food industries. A few species of unicellular algae (called carotenogenic microalgae ) mainly the representatives of Chlorophyta, are among the richest biological source of carotenoids such as β-carotene and astaxanthin . This chapter covers the mass cultivation of the microalgae in closed systems (photobioreactors) for the production of value-added carotenoids. The biochemistry and regulation of the biosynthes is of secondary carotenoids are considered together with the biotechnology of most important carotenogenic microalgae species. Special attention is paid to the real-time optical monitoring of carotenoid accumulation in microalgal cultures.

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Flat panel airlift photobioreactors for cultivation of vegetative cells of microalga Haematococcus pluvialis

Cited by 64 publications

References 20 publications

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Producción a La Intemperie De Un Consorcio Microbiano Fotosintético Fijador De Nitrógeno en Cultivo Por Lote Y Semicontinuo en Un Fotobiorreactor De Puente Aéreo De Cara Plana

Production of Carotenoids Using Microalgae Cultivated in Photobioreactors

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