Flavan-3-ols Having a .GAMMA.-Lactam from the Roots of Actinidia arguta Inhibit the Formation of Advanced Glycation End Products in Vitro

Jang, Dae Sik; Lee, Ga Young; Lee, Yun Mi; Kim, Young Sook; Sun, Hang; Kim, Dong‐Hee; Kim, Jin Sook

doi:10.1248/cpb.57.397

Cited by 68 publications

(46 citation statements)

References 25 publications

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“…The 1 H-and 13 C-NMR spectral data revealed that these newly isolated compounds are 6-and 8-(2-pyrrolidinone-5-yl)-(-)-epicatechins, which may be produced by condensation between (-)-epicatechin and 5-hydroxypyrrolidin-2-one under acidic conditions. Proanthocyanidin B-4 and the two novel compounds showed a significant activity against AGEs formation with IC50 values for proanthocyanidin B-4 of 10.1 µM, which is lower than that of the well known glycation inhibitor AG [148].…”

Section: The Most Important Catechins Of Green Tea Are (-)-Epicatechimentioning

confidence: 80%

“…They also exerted various protective effects on glucose consumption impaired by high MGO concentrations through potential interaction with proteins involved in insulin signaling pathways [147]. Proanthocyanidin B-4 and two more nitrogen containing flavonoids were identified for the first time in Actinidia arguta [148]. The N-containing flavonoids comprise a very small class of natural products, which have been only rarely isolated from natural sources.…”

Section: The Most Important Catechins Of Green Tea Are (-)-Epicatechimentioning

confidence: 99%

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Plant-Derived Agents with Anti-Glycation Activity

Odjakova¹,

Popova²,

Sharif³

et al. 2012

Glycosylation

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Section: The Most Important Catechins Of Green Tea Are (-)-Epicatechimentioning

confidence: 80%

Section: The Most Important Catechins Of Green Tea Are (-)-Epicatechimentioning

confidence: 99%

Plant-Derived Agents with Anti-Glycation Activity

Odjakova¹,

Popova²,

Sharif³

et al. 2012

Glycosylation

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“…In addition, isoquercitrin also showed outstanding antioxidant activity in yeast cells by increasing superoxide dismutase (SOD) activity (Silva et al, 2009), as well as free radical scavenging properties (Panda & Kar, 2007). Recent reports have showed that flavonoids inhibit the formation of AGEs Sengupta, Uematsu, Jacobsson & Swenson, 2006;Urios, Grigorova-Borsos & Sternberg, 2007;Jang et al, 2009). Therefore, the antioxidative effects of isoquercitrin and hyperin are apparently, at least in part, involved in AGEinhibitory mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Protective activity of flavonoid and flavonoid glycosides against glucose-mediated protein damage

Kim¹,

Lee

Yokozawa

et al. 2011

Food Chemistry

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“…Our previous studies identified several single compounds and extracts from natural products that inhibited AGE formation. 18,[20][21][22] To identify agents that disrupt AGE-induced protein crosslinking in vitro, candidate compounds were added to a collagen-coated surface that had been pretreated with AGE-BSA; AGE-collagen cross linking was detected using specific anti-AGE and horseradish peroxidase-linked antibodies. The AGE cross-linking disruptor N-phenacylthiazolium bromide (PTB) reacts with and cleaves covalent AGE-derived protein crosslinks.…”

Section: Discussionmentioning

confidence: 99%

Screening System of Blocking Agents of the Receptor for Advanced Glycation Endproducts in Cells Using Fluorescence

Jung

Kim

2012

Biological & Pharmaceutical Bulletin

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Activation of the receptor for advanced glycation endproducts (RAGE) triggers cellular responses implicated in the pathogenesis of diabetic complications; blockade of RAGE has been shown to inhibit the development of diabetic complications. To develop a screening system to identify novel disruptors of advanced glycation endproducts (AGE)-RAGE binding, we used an AGE-RAGE binding system in RAGE-overexpressing cells; test compounds were screened using this system. To construct human RAGE-overexpressing cells, mouse mesangial cells (MMCs) were stably transfected with the pcDNA-human RAGE (hRAGE) vector and selected under 1 mg/mL gentamicin (G418). RAGE expression in hRAGE-overexpressing MMCs was analyzed by Western blotting with specific RAGE antibody. To identify novel disruptors of AGE-RAGE binding, 50 single compounds and AGE-bovine serum albumin (BSA)-Alexa 488 (AGE-BSA labeled with Alexa 488) were treated to the hRAGE-overexpressing MMCs. Nonbinding AGE-BSA-Alexa 488 was washed and fluorescence measured by microtiter plate reader (excitation wavelength, 485 nm; emission wavelength, 528 nm). In hRAGE-overexpressing cells, only treatment with AGE-BSA-Alexa 488 significantly increased fluorescence intensity in a dose-dependent manner. Of 50 compounds tested, genistein disrupted AGE-RAGE binding in a dose-dependent manner. This AGE-RAGE binding system using AGE-BSA-Alexa 488 in hRAGE-overexpressing cells was suitable for screening of agents that disrupt AGE-hRAGE binding. Key words advanced glycation endproduct; receptor for advanced glycation endproduct; screeningChronic hyperglycemia causes increased formation of advanced glycation endproducts (AGEs) in tissues of individuals with diabetes. The formation and accumulation of AGEs can accelerate the development of diabetic vascular complications.1) Approaches for the development of drugs either to prevent or treat diabetic complications have focused on inhibition of AGE formation, suppression of AGE receptor (RAGE) expression or its downstream pathways, and blockade of the AGE-RAGE interaction.2-4) Inhibitors of AGE formation such as aminoguanidine (Pimagedine) and pyridoxamine (Pyridorin) prevent diabetic nephropathy by inhibiting albuminuria, glomerular hypertrophy, and mesangial expansion in patients with type 1 diabetes and in animal models. 2,5,6) The AGE-protein crosslinker breaker ALT-711 (Alagebrium) improves endothelial dysfunction in patients with isolated systolic hypertension and decreases left ventricular mass in patients with diastolic heart failure. [7][8][9] In our previous study, 700 plant extracts and phytochemicals were tested for inhibition of AGE formation by incubating glucose and bovine serum albumin (BSA) for 14 d; several extracts were shown to prevent diabetic complications, specifically nephropathy, cataract formation, and retinopathy in experimental animal models. [10][11][12]

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Flavan-3-ols Having a .GAMMA.-Lactam from the Roots of Actinidia arguta Inhibit the Formation of Advanced Glycation End Products in Vitro

Cited by 68 publications

References 25 publications

Plant-Derived Agents with Anti-Glycation Activity

Plant-Derived Agents with Anti-Glycation Activity

Protective activity of flavonoid and flavonoid glycosides against glucose-mediated protein damage

Screening System of Blocking Agents of the Receptor for Advanced Glycation Endproducts in Cells Using Fluorescence

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