Flavobacterium columnare colony types: Connection to adhesion and virulence?

Kunttu, Heidi; Suomalainen, Lotta-Riina; Jokinen, Eeva; Valtonen, E. T.

doi:10.1016/j.micpath.2008.10.001

Cited by 49 publications

(93 citation statements)

References 46 publications

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“…No correlation was observed between the adhesion of groups I and II and the genomovar, virulence, or the date or source of isolation. Previous studies have shown that adhesion to host tissues by F. columnare was correlated with virulence (6, 53) while attachment to polystyrene was not (32,37). We have further confirmed this by using a broader diversity of isolates of F. columnare.…”

Section: Discussionsupporting

confidence: 83%

“…Previous studies of F. columnare adhesion mechanisms focused on host-pathogen interactions by using in vivo models (20) or proxies such as explants of living tissues (19) or polystyrene plates (32). Herein, we have shown that F. columnare can colonize a variety of man-made materials and have characterized the progression of biofilm development on different surfaces.…”

Section: Discussionmentioning

confidence: 91%

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Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Cai

Fuente

Arias

2013

Appl Environ Microbiol

102

105

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bFlavobacterium columnare is a bacterial fish pathogen that affects many freshwater species worldwide. The natural reservoir of this pathogen is unknown, but its resilience in closed aquaculture systems posits biofilm as the source of contagion for farmed fish. The objectives of this study were (i) to characterize the dynamics of biofilm formation and morphology under static and flow conditions and (ii) to evaluate the effects of temperature, pH, salinity, hardness, and carbohydrates on biofilm formation. Nineteen F. columnare strains, including representatives of all of the defined genetic groups (genomovars), were compared in this study. The structure of biofilm was characterized by light microscopy, confocal laser scanning microscopy, and scanning electron microscopy. F. columnare was able to attach to and colonize inert surfaces by producing biofilm. Surface colonization started within 6 h postinoculation, and microcolonies were observed within 24 h. Extracellular polysaccharide substances and water channels were observed in mature biofilms (24 to 48 h). A similar time course was observed when F. columnare formed biofilm in microfluidic chambers under flow conditions. The virulence potential of biofilm was confirmed by cutaneous inoculation of channel catfish fingerlings with mature biofilm. Several physicochemical parameters modulate attachment to surfaces, with the largest influence being exerted by hardness, salinity, and the presence of mannose. Maintenance of hardness and salinity values within certain ranges could prevent biofilm formation by F. columnare in aquaculture systems.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 91%

Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment

Cai

Fuente

Arias

2013

Appl Environ Microbiol

102

105

View full text Add to dashboard Cite

show abstract

“…Hence, attachment, although necessary for invasion, cannot be directly correlated with virulence of F. columnare. Our in vivo results could explain discrepancies among various research laboratories conducting adhesion studies with F. columnare where only one time point was assayed (Decostere et al 1999c, Kunttu et al 2009). Channel catfish reduced the number of gill-adhered genomovar I cells by 4 orders of magnitude.…”

Section: Discussionmentioning

confidence: 89%

“…Several studies have evaluated adhesion of high and low virulence strains of Flavobacterium columnare to host tissues (Decostere et al 1999a, Nematollahi et al 2003, Kunttu et al 2009), but to our knowledge this is the first study comparing strains from both genomovars. Our in vivo results showed significantly different attachment to channel catfish gill by F. columnare strains from different genomovars.…”

Section: Discussionmentioning

confidence: 99%

“…It is plausible that genomovar II strains could more efficiently adhere to the epithelial tissues and mucus coverings of catfishes such that intense infections and subsequent manifestation of colum naris disease are more likely. However, the relationship between adhesion of F. columnare cells and virulence is not clear at this point with some studies supporting a positive correlation (Decostere et al 1999a,b) while others found no such association (Kunttu et al 2009). Herein, we studied the dynamics of adhesion of genomovar I and II strains in channel catfish and zebrafish Danio rerio (Hamilton, 1822) after exposure to F. columnare by immersion bath.…”

Section: Introductionmentioning

confidence: 91%

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