FlavoDb: a web-based chemical repository of flavonoid compounds

Kolte, Baban; Londhe, Sanjay R.; Bagul, Kamini T; Pawnikar, Shristi; Goundge, Mayuri B.; Gacche, Rajesh N.; Meshram, Rohan J.

doi:10.1007/s13205-019-1962-7

Cited by 3 publications

(1 citation statement)

References 59 publications

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“…For Molecular docking, Autodock v4.2 [18], PatchDock [17] and Autodock Vina [16] were used. The enzyme (PDB ID: 3EST) and inhibitor SDF (PubChem ID: 5281605; FlavoDb Accession Number: FD014805 [40]) files were obtained and processed for Molecular Docking using Autodock Tools v4.2 via PyRx v0.8 [36]. Autogrid was set to 'maximized' for Blind Docking; for active site docking the grid size for was set to cover the active site residues.…”

Section: Molecular Docking Visualisation and Admet Testingmentioning

confidence: 99%

Molecular Elucidation of Pancreatic Elastase Inhibition by Baicalein

Ghosh

Bansode

Joshi

et al. 2020

Preprint

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The serine protease, elastase exists in various forms and plays diverse roles in the human body. Pharmacological inhibition of elastase has been investigated for its therapeutic role in managing conditions such as diabetes, pneumonia and arthritis. Sivelestat, a synthetic molecule, is the only elastase inhibitor to have been approved by any major drug regulatory authority (PMDA, in this case), but still has failed to attain widespread clinical usage owing to its high price, cumbersome administration and obscure long-term safety profile. In order to find a relatively better-suited alternative, screening was conducted using plant flavonoids, which yielded Baicalein, a molecule that showed robust inhibition against Pancreatic Elastase inhibition (IC50: 3.53 micromolar). Other than having an IC50 almost 1/5th of that of sivelestat, baicalein is also cheaper, safer and easier to administer. While Microscale thermophoresis validated baicalein-elastase interaction, enzyme-kinetic studies, molecular docking and molecular dynamic simulation revealed the mode of inhibition to be non-competitive. Baicalein exhibited binding to a distinct allosteric site on the enzyme. The current study demonstrates the elastase inhibition properties of baicalein in an in-vitro and in-silico environment.

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