Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

Victório, Cristiane Pimentel; Lage, Celso Luiz Salgueiro; Küster, Ricardo Machado

doi:10.26850/1678-4618eqj.v34.1.2009.p19-24

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…In our work; the aqueous fraction rich in phenols was more effective. This finding suggests that solvent extraction technique affects secondary metabolites extraction and bioactivity [30] .…”

Section: Discussion Of the Antibacterial Activitymentioning

confidence: 88%

Bioguided isolation, structure elucidation and evaluation of new antimicrobial flavonoid from Carica papaya leaves

Nicolas¹,

Alvine²,

Gaëtan³

et al. 2021

J Pharmacogn Phytochem

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The emergence of antimicrobial resistance is a global concern, particularly in developing countries. The aims of this study were to isolate and identify antimicrobial fractions of C. papaya based on their antimicrobial activity and evaluate the antimicrobial effects of flavonoid to provide natural resources against S. enteretidis, S. typhi, E. coli, S. flexneri and K. pneumonia using the broth microdilution method. The aqueous fraction showed high inhibition potentials against all the tested bacteria with a Minimum Inhibitory Concentration value of 500 µg/mL. Subsequent phytochemical investigation of aqueous fraction by bioactive-guided column chromatography identified by 1 H NMR, 13 C NMR and ESI-MS experiments, one new flavonoid named 6"-O-acetyl-3'-O-methyl orientin. The antimicrobial effects of flavonoid against the same pathogens were close to those of the aqueous fraction. This result showed that 6"-O-acetyl-3'-O-methyl orientin is responsible for the antimicrobial activity of Carica papaya leaves and may be considered as antimicrobial lead compound.

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Section: Discussion Of the Antibacterial Activitymentioning

confidence: 88%

Bioguided isolation, structure elucidation and evaluation of new antimicrobial flavonoid from Carica papaya leaves

Nicolas¹,

Alvine²,

Gaëtan³

et al. 2021

J Pharmacogn Phytochem

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“…purpurata crude extracts inhibited C. neoformans, T. rubrum and M. canis fungi. Hydroalcoholic extracts and polar fractions, such as ethyl acetate and butanol, of A. zerumbet and A. purpurata contain high concentrations of phenolic compounds, such as flavonoids [20,21,28] , which present antimicrobial properties [29] . species was also verified by Habsah et al [30] , different from the findings reported by Mathew and Victório [5] cited above.…”

Section: Resultsmentioning

confidence: 99%

“…For antimicrobial activity, crude extracts were tested, along with hexane, dichloromethane, ethyl acetate and nbutanol fractions. As standard, the flavonoid rutin (Merck ® ) was used by its previously identification in extracts of A. zerumbet and A. purpurata [20,21] . The nonpolar samples were initially solubilized in DMSO (50 mg/ml) and diluted in sterile distilled water 1:3 (1 part of the sample in DMSO + 2 parts of sterile distilled water) before being dropped onto the surface of inoculated medium.…”

Section: Methicillin-resistant Staphylococcus Aureus (Mrsa) Enterococ...mentioning

confidence: 99%

In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Victório

Silva

Alviano

et al. 2021

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This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.

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“…Burtt & R.M. Sm (Zingiberaceae), conhecida popularmente no Brasil como colônia, tem amplo emprego na medicina tradicional e popular como diurética e anti-hipertensiva (KRESS; PRINCE; WILLIAMS, 2002;MORITA et al, 1996;MPALANTINOS et al, 1998;VICTÓRIO et al, 2009). A espécie vem sendo utilizada em diversos programas de fitoterapia em saúde pública, cultivada em hortos medicinais e Arranjos Produtivos Locais de plantas medicinais e fitoterápicos apoiados pelo Ministério da Saúde.…”

Section: Introductionunclassified

“…Estudos farmacológicos associam as atividades anti-hipertensiva e diurética com a presença de compostos fenólicos, com destaque para os flavonoides (MPALANTINOS et al, 1998;KUSTER, 2009).…”

Section: Introductionunclassified

Influência da técnica de extração e do tamanho de partícula do material vegetal no teor de compostos fenólicos totais da tintura das folhas de Alpinia zerumbet

et al. 2017

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Influência da técnica de extração e do tamanho de partícula do material vegetal no teor de compostos fenólicos totais da tintura das folhas de Alpinia zerumbetIgor Cardoso et al AbstractThe specie Alpinia zerumbet (Zingiberaceae) has been use in the traditional and folk medicine as a diuretic and antihypertensive. The Herbal Medicines Formulary of the Brazilian Pharmacopoeia lists Alpinia zerumbet for the treatment of mild hypertension. This study aims to evaluate the influence of the extraction technique and particle size of the plant material in the extraction of total phenolic compounds from Alpinia zerumbet leaves. Dried leaves were extracted using a mixture of ethanol:water (7:3, v/v) combining four techniques (percolation, static maceration, ultrasound and dynamic maceration) with four ranges of particle size (not sieved, 16-32 mesh, 32-60 mesh and 60-100 mesh), totaling 16 extractions. The total phenolic compounds were quantified using the spectrophotometric method of Folin-Denis. Percolation was shown to be the most efficient method, followed by dynamic maceration, ultrasound and finally, the less efficient method, static maceration. The particle size of the plant material was not significant in the extraction of total phenolics. The percolation and the particle size ranges from 60 to 100 mesh proved to be the most efficient combination evaluated in this study.

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Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

Cited by 6 publications

References 9 publications

Bioguided isolation, structure elucidation and evaluation of new antimicrobial flavonoid from Carica papaya leaves

Bioguided isolation, structure elucidation and evaluation of new antimicrobial flavonoid from Carica papaya leaves

In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Influência da técnica de extração e do tamanho de partícula do material vegetal no teor de compostos fenólicos totais da tintura das folhas de Alpinia zerumbet

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