Flavonoids and oxidative stress in Drosophila melanogaster

Sotibrán, América Nitxin Castañeda; Ordaz-Téllez, María Guadalupe; Rodríguez-Arnáiz, Rosario

doi:10.1016/j.mrgentox.2011.08.005

Cited by 20 publications

(9 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kaempferol is a common flavonoid that is found in vegetables, fruits and tea (25,26). Although kaempferol reportedly has anti-proliferative and cytotoxic effects on human lung cancer cells, to the best of our knowledge, no study has previously demonstrated that it can enhance radiosensitivity (27,28).…”

Section: Discussionmentioning

confidence: 99%

Radiosensitization of non-small cell lung cancer by kaempferol

Kuo

Tsai

et al. 2015

Oncology Reports

View full text Add to dashboard Cite

Abstract. The aim of the present study was to determine whether kaempferol has a radiosensitization potential for lung cancer in vitro and in vivo. The in vitro radio-sensitization activity of kaempferol was elucidated in A-549 lung cancer cells by using an MTT (3-(4 5-dimethylthiazol-2-yl)-25-diphenyl tetrazolium bromide) assay, cell cycle analysis and clonogenic assay. The in vivo activity was evaluated in the BALB/c nude mouse xenograft model of A-549 cells by hematoxylin and eosin staining and immunohistochemistry, and the tumor volume was recorded. Protein levels of the apoptotic pathway were detected by western blot analysis. Treatment with kaempferol inhibited the growth of A-549 cells through activation of apoptotic pathway. However, the same doses did not affect HFL1 normal lung cell growth. Kaempferol induced G2/M cell cycle arrest and the enhancement of radiation-induced death and clonogenic survival inhibition. The in vivo data showed that kaempferol increased tumor cell apoptosis and killing of radiation. In conclusion, the findings demonstrated that kaempferol increased tumor cell killing by radiation in vitro and in vivo through inhibition of the AKT/PI3K and ERK pathways and activation of the mitochondria apoptosis pathway. The results of the present study provided solid evidence that kaempferol is a safe and potential radiosensitizer. IntroductionLung cancer is the leading cause of cancer-associated mortalities worldwide, with an estimated 1.61 million new cases and 1.38 million mortailies in 2008 (1,2). Approximately 85% of primary lung cancer patients have non-small-cell lung cancer (NSCLC) (3). Surgery is the standard treatment for early-stage NSCLC. However, the majority of patients diagnosed at an advanced stage are unsuitable for surgical resection or extensive mediastinal lymphadenopathy (4,5). Chemo-and radiotherapy are the current standard of care for patients with unresectable advanced NSCLC (6,7). However, there are several limiting factors of chemo-and radiotherapy, including dose tolerance limitation of normal tissue and tumor radioresistance (8). Accordingly, identifying effective agents for enhancing tumor sensitivity to radiation and reducing adverse effects on normal tissues are crucial.Acting as radiosensitizers, drugs can accelerate the killing of cancer cells by increasing the effectiveness of radiation with little effect on normal cells (9). The PI3K/Akt and ERK pathways play an essential role in confirming sensitivity or resistance to radiation by inhibiting a survival pathway that induces cell apoptosis (10,11). Previous studies also showed that treatment with the ERK inhibitor can reduce radioresistance and suppressed activation of PI3K/Akt can promote radiosentization (12,13). Inhibition of growth of cancer cells and the induction of apoptosis are important determinants of the response to anticancer therapy (14).Bioflavonoids, which are the phytochemicals that are abundant in a variety of plants have a vital role in cancer prevention as they can scavenge free radicals (1...

show abstract

Section: Discussionmentioning

confidence: 99%

Radiosensitization of non-small cell lung cancer by kaempferol

Kuo

Tsai

et al. 2015

Oncology Reports

View full text Add to dashboard Cite

show abstract

“…Epidemiological evidence suggests that a high intake of bioactive food is associated with a lower risk of developing chronic diseases like obesity [ 20 ]. Bioactive foods may influence the physiological and cellular activities of oxidative pathways and in recent years the attention has been focused on a class of secondary metabolites present in plant foods called flavonoids that seem to possess beneficial properties in preventing chronic diseases [ 21 , 22 ]. The possible health benefits of flavonoids are linked to their potent antioxidant and free radical scavenging activities demonstrated in vitro and in vivo using different animal models [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Anthocyanins Function as Anti-Inflammatory Agents in a Drosophila Model for Adipose Tissue Macrophage Infiltration

Valenza

Bonfanti

Pasini

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

Epidemiological and preclinical studies have demonstrated that bioactive foods like flavonoids, polyphenolic compounds derived from fruits and vegetables, exert a protective action against obesity, cardiovascular disorders, and Adipocyte Tissue Macrophage infiltration (ATM). All these pathologies are characterized by increase in reactive oxygen species (ROS) and in proinflammatory cytokines that have been shown to favor the migration of immune cells, particularly of macrophages, in metabolically active organs like the liver and adipose tissue, that in Drosophila are constituted by a unique organ: the fat body. This study, using a unique Drosophila model that mimics human ATM, reveals the beneficial effects of flavonoids to reduce tissue inflammation. Our data show that anthocyanin-rich food reduces the number of hemocytes, Drosophila macrophages, infiltrating the fat cells, a process that is associated with reduced production of ROS and reduced activation of the JNK/SAPK p46 stress kinase, suggesting a fundamental function for anthocyanins as antioxidants in chronic inflammation and in metabolic diseases.

show abstract

“…Quercetin (a penta hydroxyl flavone) is a plant-derived bioflavonoid (from tomatoes and onions) which is recently gaining scientific interest for its antioxidant, free radical scavenging, anti-angiogenesis, antitumor, and anti-inflammatory properties ( Boesch-Saadatmandi et al 2012 , Kobori et al 2015 , Zhao et al 2016 ). Quercetin at the dose used in this study (0.1%) was not found to be toxic in flies ( Sotibran et al 2011 ). This bioflavonoid exhibits therapeutic potential in various ailments like cancer, coronary artery, asthma and Alzheimer disease ( Boesch-Saadatmandi et al 2012 , Kobori et al 2015 , Zhao et al 2016 ).…”

mentioning

confidence: 65%

Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster

Subramanian¹,

Kaliyamoorthy²,

Jayapalan³

et al. 2017

Journal of Insect Science

View full text Add to dashboard Cite

Numerous biological processes are governed by the biological clock. Studies using Drosophila melanogaster (L.) are valuable that could be of importance for their effective applications on rodent studies. In this study, the beneficial role of quercetin (a flavonoid) on H2O2 induced stress in D. melanogaster was investigated. D. melanogaster flies were divided into four groups (group I – control, group II – H2O2 (acute exposure), group III – quercetin, and group IV – quercetin + H2O2 treated). Negative geotaxis assay, oxidative stress indicators (protein carbonyls, thiobarbituric reactive substances [TBARS]), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione-S-transferase [GST], glutathione peroxidase, and reduced glutathione [GSH]) were measured at 4 h intervals over 24 h and temporal expression of heat shock protein-70 (Hsp70), Upd1 (homolog of IL-6 in Drosophila), and nitric oxide synthase (Nos) was analyzed by Western blotting. Groups II and IV showed altered biochemical rhythms (compared with controls). Decreased mesor values of negative geotaxis, SOD, CAT, GST, and GSH were noticed in H2O2, increased mesor of oxidative stress indicators (TBARS and protein carbonyl content) and a reversibility of the rhythmic characteristics were conspicuous after quercetin treatment. The expression levels of Hsp70, Upd1, and Nos were noticeably maximum at 04:00. Significant elevation of expression by H2O2 was nearly normalized by quercetin treatment. The possible mechanism by which quercetin modulates oxidant–antioxidant imbalance under oxidative stress could be ascribed to the modulation of the rhythmic properties. Our results will be helpful to understand the molecular interlink between circadian rhythm and oxidative stress mechanism.

show abstract

Flavonoids and oxidative stress in Drosophila melanogaster

Cited by 20 publications

References 52 publications

Radiosensitization of non-small cell lung cancer by kaempferol

Radiosensitization of non-small cell lung cancer by kaempferol

Anthocyanins Function as Anti-Inflammatory Agents in a Drosophila Model for Adipose Tissue Macrophage Infiltration

Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster

Contact Info

Product

Resources

About