Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster

Abstract

Numerous biological processes are governed by the biological clock. Studies using Drosophila melanogaster (L.) are valuable that could be of importance for their effective applications on rodent studies. In this study, the beneficial role of quercetin (a flavonoid) on H2O2 induced stress in D. melanogaster was investigated. D. melanogaster flies were divided into four groups (group I – control, group II – H2O2 (acute exposure), group III – quercetin, and group IV – quercetin + H2O2 treated). Negative geotaxis assay, oxidative stress indicators (protein carbonyls, thiobarbituric reactive substances [TBARS]), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione-S-transferase [GST], glutathione peroxidase, and reduced glutathione [GSH]) were measured at 4 h intervals over 24 h and temporal expression of heat shock protein-70 (Hsp70), Upd1 (homolog of IL-6 in Drosophila), and nitric oxide synthase (Nos) was analyzed by Western blotting. Groups II and IV showed altered biochemical rhythms (compared with controls). Decreased mesor values of negative geotaxis, SOD, CAT, GST, and GSH were noticed in H2O2, increased mesor of oxidative stress indicators (TBARS and protein carbonyl content) and a reversibility of the rhythmic characteristics were conspicuous after quercetin treatment. The expression levels of Hsp70, Upd1, and Nos were noticeably maximum at 04:00. Significant elevation of expression by H2O2 was nearly normalized by quercetin treatment. The possible mechanism by which quercetin modulates oxidant–antioxidant imbalance under oxidative stress could be ascribed to the modulation of the rhythmic properties. Our results will be helpful to understand the molecular interlink between circadian rhythm and oxidative stress mechanism.

Hesperidin modulates the rhythmic proteomic profiling in Drosophila melanogaster under oxidative stress

“…Hesperidin on the other hand, display antioxidative properties by scavenging of free radicals (Garg et al, 2001), restrain free radical synthesis and the distribution of free radical reactions through the chelation of metal (iron and copper) ions (Andjelković et al, 2006), suppresses the superoxide‐derived Fenton reaction (Mladenka et al, 2011) and augment endogenous antioxidative defense system (Poetini et al, 2018). Previous studies had indicated that treatment with flavonoids could reverse the disturbed rhythmicity of antioxidants and biochemical indices (Subramanian et al, 2017). The data of the present study generally substantiate the previously reported finding of the rhythmic proteome profiling.…”

“…The flies were categorized into four groups (Group I: wild‐type/control; Group II: rotenone‐treated; Group III: rotenone and hesperidin treated; and Group IV: hesperidin‐treated flies). Rotenone (70 mg/unit media; 1 unit medium = 300 ml) and hesperidin (0.1 g/100 ml medium) were administered to adult male flies (10 days old) for 7 days through media (Arumugam, Jayapalan, Abdul‐Rahman, Hashim, & Subramanian, 2018; Subramanian, Kaliyamoorthy, Jayapalan, Abdul‐Rahman, & Haji Hashim, 2017; Subramanian, Prasanna, Jayapalan, Abdul Rahman, & Hashim, 2014). On the seventh day, male flies ( n = 15/group/time point) were collected from four groups at 6 h intervals (00:00, 06:00, 12:00, and 18:00) continuously over the 24‐h period.…”

“…The homogenate was then precipitated in trichloroacetic acid (20%) in cold acetone (90%) together with dithiothreitol (DTT; 20 mM) on ice (Jessie, Hashim, & Rahim, 2008). The proteomic study for the whole fly was carried out as described previously (Subramanian et al, 2017).…”

Could Hesperidin and Iron chelator be a new Therapeutic approach in Gentamicin induced Acute Kidney Injury in Rats?

“…Moreover, hesperidin up-regulates HO-1 expression through increased nuclear factor erythroid 2-related factor (Nrf2) signaling [46].The anti-inflammatory effect of HES has been chiefly associated with its antioxidant activity and ability to inactivate the proinflammatory cascade initiated by free radicals. It inhibits of nuclear factor kappa B (NF-κB) expression that triggers pro-inflammatory genes synthesis [10].…”

“…Daflon, a dietary supplement, is also a source for hesperidin [8].Hesperidin is a strong antioxidant and one of naturally occurring iron chelators [9]. In addition, it suppresses inflammation [10].…”