Flavonoids From Endophytic Bacteria of Cosmos Caudatus Kunth. Leaf as Anticancer and Antimicrobial

Abstract

Objective: The present study aimed to isolate and examine the characteristics of endophytic bacteria from kenikir (Cosmos caudatus Kunth.) leaves, to determine the endophytic bacteria capable of producing flavonoids, and to test their potency as anticancer and antimicrobial.Methods: The isolation of endophytic bacteria from C. caudatus Kunth. leaf was conducted by technical surface sterilization. Each of these isolates was produced from the insulation and then cultured on a liquid medium of 0.1% soluble starch, 0.5% peptone, and 0.15% yeast extract with a pH of 7 which have been incubated in room temperature for 5 days with agitation of 120 rpm and extracted with ethyl acetate solvent. The extract was then tested for flavonoid content using thin-layer chromatography method. The anticancer ability of flavonoids was tested by brine shrimp lethality test (BSLT) method, while that for antimicrobial, the test used paper disc method.Results: A total of 15 isolates of endophytic bacteria were successfully isolated from the kenikir leaves, but only 4 isolates produced flavonoids, among others PKM 1 (Serratia sp.), PKM 2 (Neisseria sp.), PKM 17 (Acinetobacter sp.), and PKM 19 (Yersinia sp.). BSLT test results showed that the flavonoids cause mortality in Artemia salina leach with LC50 as much as 16.736 in PKM 17, 17.267 in PKM 2, 18.672 in PKM 1, and 23.411 in PKM 19. The flavonoids also inhibited the growth of pathogens in human-based antimicrobial test results.Conclusion: Flavonoids produced by four endophytic bacterial isolates from kenikir leaves have great potential as anticancer and antimicrobial.

Synergistic Effects of Propolis Combined with 2-Phenoxyethanol and Antipyretics on the Growth of Staphylococcus aureus

“…Mirzoeva et al [24] found that some isolated propolis components, including cinnamic derivatives and flavonoids, affect the potential and mobility of bacterial membranes. Quercetin has been reported to disrupt cell membranes and inactivate extracellular proteins by forming irreversible complexes [44]. Galangin and caffeic acid derived from propolis are considered to be bacterial enzyme inhibitors [45].…”

Eksplorasi Jenis dan Pemanfaatan Tanaman Obat Di Bumi Patowonua, Kabupaten Kolaka Utara, Sulawesi Tenggara

“…Bijinya berbentuk paruh (Faiha, 2015). Kenikir mengandung senyawa turunan flavonoid salah satunya adalah quercetin yang mempu menghambat pertumbuhn bakteri gram positif dan gram negatif (Ramadhan et al, 2018). Secara empiris bunga kenikir telah digunakan masyarakat Bumi Patowonua untuk mengobati gondongan dan pembengkakan payudara dengan cara bunga kerikir yang sudah dikeringkan, ditumbuk denga halus, kemudian dicampur dengan cuka, ditempel dibagian yang sakit.…”

AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN KENIKIR (Cosmos caudatus Kunth.) TERHADAP Staphylococcus epidermidis, Pseudomonas aeruginosa, DAN BIOAUTOGRAFINYA

“…Sehingga hasil uji KLT menegaskan bahwa dalam ekstrak metanol daun kenikir mengandung flavonoid pada Rf 0,34 0,61 dan 0,77. Pada penelitian Ramadhan et al, (2018) ekstrak daun kenikir mengandung 4 isolat yang positif mengandung flavonoid. Identifikasi senyawa fenol dengan pereaksi semprot FeCl3 menunjukkan hasil positif spot yang berwarna hijau, ungu, biru atau hitam yang dilihat pada sinar tampak (Wagner and Bladt, 1996).…”