Flax lignan concentrate reverses alterations in blood pressure, left ventricular functions, lipid profile and antioxidant status in DOCA-salt induced renal hypertension in rats

Abstract

Context Earlier we reported cardioprotective, antihyperlipidemic, and in vitro antioxidant activity of flax lignan concentrate (FLC) obtained from the seeds of Linum usitatissimum L. (Linaceae). Objective To investigate the effect of FLC in deoxycorticosterone acetate (DOCA)-salt induced experimental renal hypertension in rats. Materials and methods Hypertension was induced in uninephrectomized (UNTZD) male Wistar rats (230-280 g) by injecting DOCA (25 mg/kg, subcutaneously, twice weekly) and supplementing 1% NaCl in drinking water for 5 weeks. The rats were divided in six groups. Captopril (30 mg/kg, p.o.) and FLC (200, 400 and 800 mg/kg, p.o.) were administered daily to the rats of groups III-VI, respectively, for 5 weeks. Various hemodynamic and biochemical parameters were investigated as well as histology of kidney and heart were carried out. Results In this study, the FLC (400 and 800 mg/kg) significantly (p50.01, p50.001) decreased the systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure. It also significantly (p50.01, p50.001) decreased elevated end diastolic pressure (EDP), dP/dt max and dP/dt min, organs weights (kidney and heart) and activities of hepatic, renal and cardiac marker enzymes in the serum. Furthermore, FLC (400 and 800 mg/kg) significantly (p50.01, p50.001) restored altered antioxidant status, serum electrolyte level, lipid profile values, and histological abnormalities. Captopril (30 mg/kg) showed maximum antihypertensive effect but low dose of FLC (200 mg/kg) was not enough to show the antihypertensive activity. Conclusion FLC possessed antihypertensive effect via modulation of endogenous enzymes in DOCA-salt induced renal hypertension in rats.ARTICLE HISTORY

Flaxseeds (L. Usitatissimum) attenuates blood pressure, acetylcholinesterase activity and oxidative stress in ouabain-induced hypertension in normal Wistar rats

“…These results confirm those reported by Ghule et al (2012) who showed that an ethanolic extract of flaxseed attenuates the rise in blood pressure. The similar results were found also by Sawant and Bodhankar(2016), in lignane extract (FLC)-treated hypertensive rats obtained from L. usitatissimum L. (Linaceae) seeds. In fact, the antihypertensive effect obtained is attributed to its opulence in lignan composition such as SDG.…”

“…Presently, flaxseed has been drawing increasing scientific attention for its potential role in preventing cardiovascular diseases (CVD) (Tirgar and Kalpesh, 2017). However, the flaxseeds possess anti-hypertensive (Ghule et al , 2012; Sawant and Bodhankar, 2016) and antihyperlipidemic properties (Dali et al , 2019; Zanwar et al , 2014), that might be beneficial to prevent the atherosclerotic plaques (Francis et al , 2013). Additionally, they also possess anti-oxidant (Cameron et al , 2013) and anti-inflammatory activities (Kaithwas and Majumdar, 2013).…”

Linseed Components Are More Effective Than Whole Linseed in Reversing Diet-Induced Metabolic Syndrome in Rats

“…Both linseed oil and SDG prevented the increase in systolic blood pressure in rats with metabolic syndrome induced by feeding with 30% fructose, likely due to decreased oxidative stress [34]. In deoxycorticosterone acetate (DOCA)-salt hypertensive rats, linseed lignan concentrate lowered blood pressure, and improved antioxidant status, serum electrolytes, and lipid profiles [35]. A lignan-enriched linseed powder reduced blood pressure, body weight, and fat accumulation, and improved lipid profiles in rats fed a high-fat and high-fructose diet [36].…”

Dietary Flaxseed as a Strategy for Improving Human Health

“…Alternatively, in animal studies, flaxseed protein hydrolysates and flax oil decreased blood pressure in the spontaneously hypertensive rat in acute or chronic conditions, respectively [26,27]. As well, a flaxseed lignan concentrate reduced blood pressure in the deoxycorticosterone acetate (DOCA-salt) induced renal hypertensive rats [28] and in the high fat, high sugar-fed rat [29].…”