Flexible loops of thread-like micelles are formed upon interaction of l-α-dimyristoyl-phosphatidylcholine with the biosurfactant surfactin as revealed by cryo-electron tomography

Boettcher, Christoph; Kell, Henny; Holzwarth, Josef F.; Vater, Joachim

doi:10.1016/j.bpc.2010.03.006

Cited by 13 publications

(7 citation statements)

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“…Sánchez et al ( 2007 ) have also observed three-size range rhamnolipid aggregates depending on the biosurfactant concentration, namely the 43–66 nm micelles coexisted with small (350–550 nm) aggregates at low concentrations, which were replaced by large (>1500 nm) aggregates at higher rhamnolipid concentrations. Many authors reported the spontaneous formation of higher order aggregates (having different shapes and sizes) from monodisperse micelles at increasing biosurfactant concentrations (Zhou et al 2004 ; Pornsunthorntawee et al 2009 ; Boettcher et al 2010 ; Song et al 2013 ) and the mechanisms of such micelle-to-vesicle transition are reviewed by Svenson ( 2004 ). Apparently, different Rhodococcus biosurfactant structures formed, depending on the concentration used, on the polystyrene surface would modify differently its physicochemical properties, thus leading to diverse anti-adhesive effects.…”

Section: Discussionmentioning

confidence: 99%

Diverse effects of a biosurfactant from Rhodococcus ruber IEGM 231 on the adhesion of resting and growing bacteria to polystyrene

et al. 2016

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This study evaluated the effects of a trehalolipid biosurfactant produced by Rhodococcus ruber IEGM 231 on the bacterial adhesion and biofilm formation on the surface of polystyrene microplates. The adhesion of Gram-positive (Arthrobacter simplex, Bacillus subtilis, Brevibacterium linens, Corynebacterium glutamicum, Micrococcus luteus) and Gram-negative (Escherichia coli, Pseudomonas fluorescencens) bacteria correlated differently with the cell hydrophobicity and surface charge. In particular, exponentially growing bacterial cells with increased hydrophobicities adhered stronger to polystyrene compared to more hydrophilic stationary phase cells. Also, a moderate correlation (0.56) was found between zeta potential and adhesion values of actively growing bacteria, suggesting that less negatively charged cells adhered stronger to polystyrene. Efficient biosurfactant concentrations (10–100 mg/L) were determined, which selectively inhibited (up to 76 %) the adhesion of tested bacterial cultures, however without inhibiting their growth. The biosurfactant was more active against growing bacteria rather than resting cells, thus showing high biofilm-preventing properties. Contact angle measurements revealed more hydrophilic surface of the biosurfactant-covered polystyrene compared to bare polystyrene, which allowed less adhesion of hydrophobic bacteria. Furthermore, surface free-energy calculations showed a decrease in the Wan der Waals (γLW) component and an increase in the acid-based (γAB) component caused by the biosurfactant coating of polysterene. However, our results suggested that the biosurfactant inhibited the adhesion of bacteria independently on their surface charges. AFM scanning revealed three-type biosurfactant structures (micelles, cord-like assemblies and large vesicles) formed on glass, depending on concentrations used, that could lead to diverse anti-adhesive effects against different bacterial species.

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Section: Discussionmentioning

confidence: 99%

Diverse effects of a biosurfactant from Rhodococcus ruber IEGM 231 on the adhesion of resting and growing bacteria to polystyrene

et al. 2016

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“…Treatment with antibiotics is the most effective procedure for eliminating or suppressing mycoplasma infection in the cell cultures. Surfactin is used commercially for curing of cell cultures and cleansing of biotechnological products of mycoplasma contamination [ 54 ]. In general, antibiotic therapies are successful in long lasting successful decontamination and do not show undesirable side effects/cytotoxic effects on eukaryotic cells [ 55 , 56 ].…”

Section: Applications Of Lipopeptidesmentioning

confidence: 99%

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

Meena

Kanwar

2015

BioMed Research International

340

203

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A lot of crops are destroyed by the phytopathogens such as fungi, bacteria, and yeast leading to economic losses to the farmers. Members of the Bacillus genus are considered as the factories for the production of biologically active molecules that are potential inhibitors of growth of phytopathogens. Plant diseases constitute an emerging threat to global food security. Many of the currently available antimicrobial agents for agriculture are highly toxic and nonbiodegradable and thus cause extended environmental pollution. Moreover, an increasing number of phytopathogens have developed resistance to antimicrobial agents. The lipopeptides have been tried as potent versatile weapons to deal with a variety of phytopathogens. All the three families of Bacillus lipopeptides, namely, Surfactins, Iturins and Fengycins, have been explored for their antagonistic activities towards a wide range of phytopathogens including bacteria, fungi, and oomycetes. Iturin and Fengycin have antifungal activities, while Surfactin has broad range of potent antibacterial activities and this has also been used as larvicidal agent. Interestingly, lipopeptides being the molecules of biological origin are environmentally acceptable.

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“…Moreover, phospholipid vesicles are easily converted into globular mixed micelles in the presence of surfactin, thereby evidencing its strong detergency power 8. 11, 12 The lipopeptide also readily adsorbs at interfaces and forms stable monomolecular films, adopting a ball‐like structure like an amphipathic nanoparticle rather than a classical surfactant 13. Molecular modeling has shown that the peptidic backbone is very flexible and that surfactin tends to self‐associate forming clusters at the water/hexane interface 14…”

Section: Introductionmentioning

confidence: 99%

Surfactin Self‐Assembles into Direct and Reverse Aggregates in Equilibrium and Performs Selective Metal Cation Extraction

2011

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On tie‐lines between water‐rich and alkane‐rich solutions, it is shown via scattering experiments that natural lipopeptide surfactin self‐assembles into direct and reverse micelles in equilibrium. Elongated direct micelles in the aqueous phase are present together with small reverse globular aggregates in the organic phase. These latter are made from hydrated surfactant without any “water pool” in the organic phase. The resulting biphasic system is used for liquid–liquid extraction of model metal cations. It is efficient with iron but not with copper or neodymium. Competitive extractions show high selectivity towards iron.

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Flexible loops of thread-like micelles are formed upon interaction of l-α-dimyristoyl-phosphatidylcholine with the biosurfactant surfactin as revealed by cryo-electron tomography

Cited by 13 publications

References 50 publications

Diverse effects of a biosurfactant from Rhodococcus ruber IEGM 231 on the adhesion of resting and growing bacteria to polystyrene

Diverse effects of a biosurfactant from Rhodococcus ruber IEGM 231 on the adhesion of resting and growing bacteria to polystyrene

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

Surfactin Self‐Assembles into Direct and Reverse Aggregates in Equilibrium and Performs Selective Metal Cation Extraction

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