Flexible method for generating needle-shaped beams and its application in optical coherence tomography

Zhao, Jingjing; Winetraub, Yonatan; Du, Lin; Vleck, Aidan Van; Ichimura, Kenzo; Huang, Cheng; Aasi, Sumaira Z.; Sarin, Kavita Y.; Zerda, Adam de la

doi:10.1364/optica.456894

Cited by 24 publications

(23 citation statements)

References 56 publications

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“…Other 3D imaging modalities can also bene t from the E2E-BPF platform. For examples, optical coherence tomography (OCT) 4,6,44,45 and photoacoustic 46 microscopy require high-resolution imaging over an extended DoF. Our BPF is expected to nd its utility in enhancing the imaging performance and broadening its applications.…”

Section: Discussionmentioning

confidence: 99%

E2E-BPF microscope: Extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution

Seong

Kim

et al. 2023

Preprint

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Several image-based biomedical diagnoses require high-resolution imaging capabilities at large spatial scales. However, conventional microscopes exhibit an inherent trade-off between depth-of-field (DoF) and spatial resolution, and thus require objects to be refocused at each lateral location, which is time-consuming. Here, we present a computational imaging platform, termed E2E-BPF microscope, which enables large-area, high-resolution imaging of large-scale objects without serial refocusing. This method involves a physics-incorporated, deep-learned design of binary phase filter (BPF) and jointly optimized deconvolution neural network, which altogether produces high-resolution, high-contrast images over extended depth ranges. We demonstrate the method through numerical simulations and experiments with fluorescently labeled beads, cells and tissue section, and present high-resolution imaging capability over a 15.5-fold larger DoF than the conventional microscope. Our method provides highly effective and scalable strategy for DoF-extended optical imaging system, and is expected to find numerous applications in rapid image-based diagnosis, optical vision, and metrology.

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Section: Discussionmentioning

confidence: 99%

E2E-BPF microscope: Extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution

Seong

Kim

et al. 2023

Preprint

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“…A number of techniques have been proposed to increase OCT imaging quality by extending the optical depth-of-field, including beam shaping to produce Bessel, 10 Bessel-like, 11 and needle beams. 12 In general, these techniques use additional hardware (phase plates, multimodal fibers segments, etc.) in the beam path to produce a nondiffracting beam with an extended focus.…”

Section: Introductionmentioning

confidence: 99%

Improved in vivo optical coherence tomography imaging of animal peripheral nerves using a prism nerve holder

et al. 2023

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. Significance Modern optical volumetric imaging modalities, such as optical coherence tomography (OCT), provide enormous information about the structure, function, and physiology of living tissue. Although optical imaging achieves lateral resolution on the order of the wavelength of light used, and OCT achieves axial resolution on a similar micron scale, tissue optical properties, particularly high scattering and absorption, limit light penetration to only a few millimeters. In addition, in vivo imaging modalities are susceptible to significant motion artifacts due to cardiac and respiratory function. These effects limit access to artifact-free optical measurements during peripheral neurosurgery to only a portion of the exposed nerve without further modification to the procedure. Aim We aim to improve in vivo OCT imaging during peripheral neurosurgery in small and large animals by increasing the amount of visualized nerve volume as well as suppressing motion of the imaged area. Approach We designed a nerve holder with embedded mirror prisms for peripheral nerve volumetric imaging as well as a specific beam steering strategy to acquire prism and direct view volumes in one session with minimal motion artifacts. Results The axially imaged volumes from mirror prisms increased the OCT signal intensity by over a 1.25-mm imaging depth in tissue-mimicking phantoms. We then demonstrated the new imaging capabilities in visualizing peripheral nerves from direct and side views in living rats and minipigs using a polarization-sensitive OCT system. Prism views have shown nerve fascicles and vasculature from the bottom half of the imaged nerve which was not visible in direct view. Conclusions We demonstrated improved OCT imaging during neurosurgery in small and large animals by combining the use of a prism nerve holder with a specifically designed beam scanning protocol. Our strategy can be applied to existing OCT imaging systems with minimal hardware modification, increasing the nerve tissue volume visualized. Enhanced imaging depth techniques may lead to a greater adoption of structural and functional optical biomarkers in preclinical and clinical medicine.

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“…16 Generally, these approaches suffer from a myriad of issues including a loss in signal-to-noise ratio (SNR), low photon efficiency, performance degradation in dense samples (e.g., skin), and artifacts caused by sidelobes or axial-intensity oscillation. Previously, we developed a needle-shaped beam (NB) with a 20× objective to extend the DOF in OCT 17,18 for dermatological imaging. However, the resolution of a 20× lens is insufficient for disease diagnosis in some human skin disorders, necessitating a beam focused via a 40× objective.…”

mentioning

confidence: 99%

“…Here, we utilize a metasurface rather than a diffractive optical element (DOE) for two reasons. First, it has several fabrication advantages: its binary structure eliminates the mask alignment issue, the single lithography utilized is more efficient compared to the fabrication of the multilevel DOE, and scalable fabrication is possible when using optical stepper lithography .…”

mentioning

confidence: 99%

Rapid Cellular-Resolution Skin Imaging with Optical Coherence Tomography Using All-Glass Multifocal Metasurfaces

et al. 2023

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Cellular-resolution optical coherence tomography (OCT) is a powerful tool offering noninvasive histologylike imaging. However, like other optical microscopy tools, a high numerical aperture (N.A.) lens is required to generate a tight focus, generating a narrow depth of field, which necessitates dynamic focusing and limiting the imaging speed. To overcome this limitation, we developed a metasurface platform that generates multiple axial foci, which multiplies the volumetric OCT imaging speed by offering several focal planes. This platform offers accurate and flexible control over the number, positions, and intensities of axial foci generated. Allglass metasurface optical elements 8 mm in diameter are fabricated from fused-silica wafers and implemented into our scanning OCT system. With a constant lateral resolution of 1.1 μm over all depths, the multifocal OCT triples the volumetric acquisition speed for dermatological imaging, while still clearly revealing features of stratum corneum, epidermal cells, and dermal-epidermal junctions and offering morphological information as diagnostic criteria for basal cell carcinoma. The imaging speed can be further improved in a sparse sample, e.g., 7-fold with a seven-foci beam. In summary, this work demonstrates the concept of metasurface-based multifocal OCT for rapid virtual biopsy, further providing insights for developing rapid volumetric imaging systems with high resolution and compact volume.

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Flexible method for generating needle-shaped beams and its application in optical coherence tomography

Cited by 24 publications

References 56 publications

E2E-BPF microscope: Extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution

E2E-BPF microscope: Extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution

Improved in vivo optical coherence tomography imaging of animal peripheral nerves using a prism nerve holder

Rapid Cellular-Resolution Skin Imaging with Optical Coherence Tomography Using All-Glass Multifocal Metasurfaces

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